Sla2 (NM_029983) Mouse Tagged ORF Clone

CAT#: MR203346

Sla2 (Myc-DDK-tagged) - Mouse Src-like-adaptor 2 (Sla2)

Product Images

Circular map for MR203346

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sla2
• Synonyms: A930009E21Rik; AI430952; SLAP-2; SLAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203346 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAGTTTGTCCAGCAGAGGGAAAACCTCCAGCCCCAGCCCCAGCTCCTCTGGTCCAGACCAGGAAC
  CCGTGTCCATGCAACCAGAAAGACGCAAGGTCACAGCTGTGGCCCTGGGCAGTTTCCCAGCAGGTGAACA
  GGCCAGACTATCTCTGAGACTCGGGGAGCCGCTGACCATCATCTCTGAGGATGGAGATTGGTGGACAGTC
  CAGTCGGAAGTCTCAGGCAGAGAGTACCACATGCCCAGTGTGTATGTGGCTAAAGTCGCCCACGGGTGGC
  TGTACGAGGGCCTGAGCCGGGAGAAAGCCGAGGAACTACTCCTGTTACCTGGGAACCCCGGAGGGGCCTT
  CCTCATCCGGGAGAGCCAGACCAGGAGAGGCTGCTATTCCCTGTCCGTCCGACTCAGCCGCCCTGCATCT
  TGGGACCGGATCAGACACTACAGGATACAGCGTCTTGACAATGGCTGGCTGTACATCTCACCTCGCCTCA
  CCTTCCCCTCACTCCACGCCTTGGTGGAGCATTACTCTGAGCTAGCAGATGGCATCTGCTGTCCCCTCAG
  GGAGCCGTGTGTCCTGCAGAAGCTTGGGCCACTACCTGGCAAAGATACACCTCCACCTGTGACTGTGCCA
  ACATCATCACTAAATTGGAAAAAGCTGGACCGCAGCCTCCTGTTTCTGGAAGCACCTGCGAGTGGGGAGG
  CATCTCTGCTCAGTGAGGGGCTCCGAGAGTCCCTCAGTTCCTACATCAGCCTGGCTGAGGACCCCTTGGA
  TGATGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203346 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSLSSRGKTSSPSPSSSGPDQEPVSMQPERRKVTAVALGSFPAGEQARLSLRLGEPLTIISEDGDWWTV
  QSEVSGREYHMPSVYVAKVAHGWLYEGLSREKAEELLLLPGNPGGAFLIRESQTRRGCYSLSVRLSRPAS
  WDRIRHYRIQRLDNGWLYISPRLTFPSLHALVEHYSELADGICCPLREPCVLQKLGPLPGKDTPPPVTVP
  TSSLNWKKLDRSLLFLEAPASGEASLLSEGLRESLSSYISLAEDPLDDA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029983
• ORF Size: 780 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029983.1, NM_029983.2, NM_029983.3, NM_029983.4, NM_029983.5, NP_084259.1
• RefSeq Size: 2508 bp
• RefSeq ORF: 780 bp
• Locus ID: 77799
• UniProt ID: Q8R4L0
• Cytogenetics: 2 H1
• MW: 28.5 kDa
• Gene Summary: Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.[UniProtKB/Swiss-Prot Function]