Macrod1 (NM_134147) Mouse Tagged ORF Clone

CAT#: MR203012

Macrod1 (Myc-DDK-tagged) - Mouse MACRO domain containing 1 (Macrod1)

Product Images

Circular map for MR203012

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Macrod1
• Synonyms: AI604841; AW743046; D930010J01Rik; Lrp16
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR203012 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGAAGGTGGACCTGAGCACCTCCACCGACTGGAAGGAGGCCAAATCCTTTCTGAAGGGCCTGA
  GTGACAAGCAGAGAGAGGAACATTACTTCTGCAAGGATTTCATTAAGCTGAAGAAGATCCCCACGTGGAA
  GGAGACAGCAAAAGGGCTGGCTGTGAAGGTGGAGGACCCCAAGTATAAGAAGGACAAGCAACTGAATGAG
  AAAATCTCCCTGTACCGTGGGGACATCACCAAGCTGGAGGTGGATGCCATTGTCAACGCTGCCAACAGCT
  CCCTGCTTGGAGGCGGAGGGGTGGACGGCTGCATTCATCGGGCCGCGGGATCCCTGCTCACGGACGAATG
  CCGCACCCTACAGAACTGCGAGACCGGCAAAGCCAAGATCACTTGCGGCTATCGGCTGCCAGCCAAGTAT
  GTCATCCACACGGTGGGGCCCATCGCCGTGGGCCAACCCACTGCCAGCCAGGCGGCCGAGCTCCGCAGCT
  GCTACTTGAGCAGCCTGGACCTGCTGCTGGAGCACCGGCTGCGCTCAGTGGCTTTCCCGTGCATCTCCAC
  AGGCGTGTTTGGCTACCCCAATGAGGAGGCTGCAGAAGTAGTGCTGGCTTCGCTGCGGGAATGGCTGGAG
  CAGCACAAGGACAAGGTGGATCGGCTCATCATCTGTGTGTTCCTGGAGAAGGACGAGGGCATTTACCGGG
  AGCGCCTGCCCCATTATTTCCCAGTAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR203012 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAKVDLSTSTDWKEAKSFLKGLSDKQREEHYFCKDFIKLKKIPTWKETAKGLAVKVEDPKYKKDKQLNE
  KISLYRGDITKLEVDAIVNAANSSLLGGGGVDGCIHRAAGSLLTDECRTLQNCETGKAKITCGYRLPAKY
  VIHTVGPIAVGQPTASQAAELRSCYLSSLDLLLEHRLRSVAFPCISTGVFGYPNEEAAEVVLASLREWLE
  QHKDKVDRLIICVFLEKDEGIYRERLPHYFPVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134147
• ORF Size: 732 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134147.1
• RefSeq Size: 1284 bp
• RefSeq ORF: 972 bp
• Locus ID: 107227
• UniProt ID: Q922B1
• Cytogenetics: 19 A
• MW: 27.1 kDa
• Gene Summary: Removes ADP-ribose from asparatate and glutamate residues in proteins bearing a single ADP-ribose moiety. Inactive towards proteins bearing poly-ADP-ribose. Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins. Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity.[UniProtKB/Swiss-Prot Function]