Tpmt (NM_016785) Mouse Tagged ORF Clone
CAT#: MR202963
- TrueORF®
Tpmt (Myc-DDK-tagged) - Mouse thiopurine methyltransferase (Tpmt)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_016785" in other vectors (4)
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USD 198.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Tpmt |
Synonyms | AW106912 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR202963 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTTGATATGAAAGAGCACCCTGATGCTGAGGTACAAAAGAACCAAGTACTAACCCTGGAAGACT GGAAAGAGAAGTGGGTGACCCGTCACATCTCATTCCATCAGGAGCAAGGGCATCAGCTATTAAAGAAGCA TTTGGATACATTTCTTAAAGGCCAGAGTGGACTGCGAGTGTTTTTCCCCCTCTGTGGAAAAGCCATTGAG ATGAAATGGTTCGCAGATCGGGGCCACACTGTAGTTGGTGTGGAAATCAGTGAAATTGGGATTCGGGAAT TTTTTGCAGAACAGAATCTTTCCTACACAGAAGAACCGCTCGCCGAAATTGCTGGAGCCAAAGTATTTAA GAGTTCTTCAGGGAGCATTTCCTTATACTGCTGCAGCATATTTGATCTTCCTAGAGCAAACATTGGCAAG TTTGACAGGATTTGGGATAGAGGAGCATTAGTGGCTATCAATCCAGGCGATCATGATCGCTATGCAGATA TAATACTGTCCCTACTGAGAAAAGAGTTTCAGTACCTCGTGGCTGTCCTTTCTTATGATCCAACAAAACA CGCAGGCCCACCATTTTATGTTCCATCTGCTGAACTTAAAAGGTTATTTGGTACAAAATGCAGCATGCAA TGCCTTGAAGAGGTAGACGCTTTGGAAGAAAGGCATAAAGCCTGGGGTCTTGACTACCTTTTTGAAAAAT TGTATCTCCTTACAGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR202963 protein sequence
Red=Cloning site Green=Tags(s) MSLDMKEHPDAEVQKNQVLTLEDWKEKWVTRHISFHQEQGHQLLKKHLDTFLKGQSGLRVFFPLCGKAIE MKWFADRGHTVVGVEISEIGIREFFAEQNLSYTEEPLAEIAGAKVFKSSSGSISLYCCSIFDLPRANIGK FDRIWDRGALVAINPGDHDRYADIILSLLRKEFQYLVAVLSYDPTKHAGPPFYVPSAELKRLFGTKCSMQ CLEEVDALEERHKAWGLDYLFEKLYLLTEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_016785 |
ORF Size | 723 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016785.1, NP_058065.1 |
RefSeq Size | 2715 bp |
RefSeq ORF | 723 bp |
Locus ID | 22017 |
UniProt ID | O55060 |
Cytogenetics | 13 24.5 cM |
MW | 27.6 kDa |
Gene Summary | Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) using S-adenosyl-L-methionine as the methyl donor (PubMed:18484748). TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC204307 | Tpmt (untagged) - Mouse thiopurine methyltransferase (Tpmt), (10ug) |
USD 300.00 |
|
MG202963 | Tpmt (tGFP-tagged) - Mouse thiopurine methyltransferase (Tpmt) |
USD 500.00 |
|
MR202963L3 | Lenti ORF clone of Tpmt (Myc-DDK-tagged) - Mouse thiopurine methyltransferase (Tpmt) |
USD 600.00 |
|
MR202963L4 | Lenti ORF clone of Tpmt (mGFP-tagged) - Mouse thiopurine methyltransferase (Tpmt) |
USD 600.00 |
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