Sigmar1 (NM_011014) Mouse Tagged Lenti ORF Clone

CAT#: MR202589L1

  • LentiORF®

Lenti ORF clone of Sigmar1 (Myc-DDK-tagged) - Mouse sigma non-opioid intracellular receptor 1 (Sigmar1)


  "NM_011014" in other vectors (6)


Interest in protein/lysate? Submit request here!

Reconstitution Protocol

USD 600.00

3 Weeks*

Size
    • 10 ug

Product Images

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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Sigmar1"

Specifications

Product Data
Type Mouse Tagged ORF Clone Lentiviral Particle
Tag Myc-DDK
Symbol Sigmar1
Synonyms mSig; O; Oprs1; Si; Sig1R; sigma1R
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(MR202589).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_011014
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_011014.2
RefSeq Size 1640 bp
RefSeq ORF 672 bp
Locus ID 18391
UniProt ID O55242
Cytogenetics 4 A5
Gene Summary This gene encodes a transmembrane protein located in the endoplasmic reticulum. The encoded protein is a receptor that binds several endogenous ligands, including N,N-dimethyltryptamine, progesterone and pregnenolone and a variety of of non-opiate compounds. The encoded protein plays a role in regulating the activity of ion channels, acting as a chaperone and protecting cells from oxidative stress. In humans, this receptor has been associated with Alzheimer's and Parkinson's diseases, stroke and numerous disease conditions such as depression, pain and addiction. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Nov 2013]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.