Guca1b (NM_146079) Mouse Tagged ORF Clone

CAT#: MR202065

Guca1b (Myc-DDK-tagged) - Mouse guanylate cyclase activator 1B (Guca1b)

Product Images

Circular map for MR202065

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Guca1b
• Synonyms: BC018258; GCAP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR202065 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCAGCAGCTGAGCTGGGAAGAGGCGGAGGCGGCCGGGGAGATGGATGTGGCGGAGTTGCAGGAGT
  GGTACAAGAAGTTTGTCGTGGAGTGTCCCAGTGGGACGCTCTTCATGCACGAGTTCAAGCGCTTCTTCAA
  GGTCACGGGCAATGAGGAGGCCAGCCAGTATGTAGAGAGCATGTTCCGAGCCTTCGACAAGAATGGGGAC
  AACACCATTGACTTCCTGGAGTATGTGGCAGCCCTGAACCTCGTGCTGAGGGGCAGCCTGGAACACAAGC
  TCAAGTGGACCTTCAAGATCTATGACAAGGACCGAAATGGCTGCATCGACCGCCTGGAACTCCTGGACAT
  TGTGGAGGCGATTTACAAGCTGAAGAAAGCCTGCCGAGCGGAGCTGGATCTGGAACACCAGGGCCAGCTG
  CTCACGCCCGAGGAGGTGGTGGACAGGATCTTCCTCCTGGTGGACGAGAATGGAGACGGCCAGCTGTCTC
  TGACCGAGTTCATCGAGGGCGCCCGTCGCGATAAGTGGGTAATGAAGATGCTACAAATGGATATCAACCC
  AGGCTGCTGGATCACGCAGCAGAGGCGGAGAAGCGCCATGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR202065 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGQQLSWEEAEAAGEMDVAELQEWYKKFVVECPSGTLFMHEFKRFFKVTGNEEASQYVESMFRAFDKNGD
  NTIDFLEYVAALNLVLRGSLEHKLKWTFKIYDKDRNGCIDRLELLDIVEAIYKLKKACRAELDLEHQGQL
  LTPEEVVDRIFLLVDENGDGQLSLTEFIEGARRDKWVMKMLQMDINPGCWITQQRRRSAMF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146079
• ORF Size: 606 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146079.1, NP_666191.1
• RefSeq Size: 1681 bp
• RefSeq ORF: 606 bp
• Locus ID: 107477
• UniProt ID: Q8VBV8
• Cytogenetics: 17 C
• MW: 23.5 kDa
• Gene Summary: Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca(2+)-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure (By similarity).[UniProtKB/Swiss-Prot Function]