Icos (NM_017480) Mouse Tagged ORF Clone

CAT#: MR202040

  • TrueORF®

Icos (Myc-DDK-tagged) - Mouse inducible T-cell co-stimulator (Icos)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_017480" in other vectors (4)


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Reconstitution Protocol

USD 300.00

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Size
    • 10 ug

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Frequently bought together (4)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Icos"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Icos
Synonyms AILIM; CCLP; CRP-1; H4; Ly115
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR202040 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCGTACTTCTGCCGTGTCTTTGTCTTCTGCTTCCTAATCAGACTTTTAACAGGAGAAATCAATG
GCTCGGCCGATCATAGGATGTTTTCATTTCACAATGGAGGTGTACAGATTTCTTGTAAATACCCTGAGAC
TGTCCAGCAGTTAAAAATGCGATTGTTCAGAGAGAGAGAAGTCCTCTGCGAACTCACCAAGACCAAGGGA
AGCGGAAATGCGGTGTCCATCAAGAATCCAATGCTCTGTCTATATCATCTGTCAAACAACAGCGTCTCTT
TTTTCCTAAACAACCCAGACAGCTCCCAGGGAAGCTATTACTTCTGCAGCCTGTCCATTTTTGACCCACC
TCCTTTTCAAGAAAGGAACCTTAGTGGAGGATATTTGCATATTTATGAATCCCAGCTCTGCTGCCAGCTG
AAGCTCTGGCTACCCGTAGGGTGTGCAGCTTTCGTTGTGGTACTCCTTTTTGGATGCATACTTATCATCT
GGTTTTCAAAAAAGAAATACGGATCCAGTGTGCATGACCCTAATAGTGAATACATGTTCATGGCGGCAGT
CAACACAAACAAAAAGTCTAGACTTGCAGGTGTGACCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR202040 protein sequence
Red=Cloning site Green=Tags(s)

MKPYFCRVFVFCFLIRLLTGEINGSADHRMFSFHNGGVQISCKYPETVQQLKMRLFREREVLCELTKTKG
SGNAVSIKNPMLCLYHLSNNSVSFFLNNPDSSQGSYYFCSLSIFDPPPFQERNLSGGYLHIYESQLCCQL
KLWLPVGCAAFVVVLLFGCILIIWFSKKKYGSSVHDPNSEYMFMAAVNTNKKSRLAGVTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017480
ORF Size 603 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017480.2
RefSeq Size 3272 bp
RefSeq ORF 603 bp
Locus ID 54167
UniProt ID Q9WVS0
Cytogenetics 1 30.6 cM
MW 22.7 kDa
Gene Summary Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes (By similarity).[UniProtKB/Swiss-Prot Function]

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