Mterfd2 (BC055877) Mouse Tagged ORF Clone

CAT#: MR201813

  • TrueORF®

Mterfd2 (Myc-DDK-tagged) - Mouse MTERF domain containing 2 (cDNA clone MGC:68077 IMAGE:4236729)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "BC055877" in other vectors (4)


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Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

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Frequently bought together (5)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Other products for "Mterfd2"

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Mterfd2
Synonyms 1810059A23Rik; 4933412H03Rik; Mterfd2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR201813 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGATGAAGAGGCGTTCCAGTTACCTTCGGAAGCTTGGCCTTGGAGAAGGGAAACTGAAGCGGGTGC
TGTCCGTTTGCCCTGAGGTTTTCACCATGCATCAGCGGGACATTGACCGAGTTGTGAAGGTCCTCAGGGA
GAAGTGCTTGTTCACGGCACAGCACATCACAGACGTTCTGCACAGATGCCCCACTGTTTTGCAGGAGGAC
CCCAACGAGCTAGAATACAAATTCCAGTATGCATATTTCAGGATGGGACTCACACATCTAGACATAGTCA
GGACGAATTTCTTACAGTATTCAATAACCAAGATCAAGCAGAGACACATTTACCTGGAGCGCCTTGGACG
GTACCAGACCCCTGATAAGAAGGGGCAGACACAGATCCCTAATCCCTCACTGAGGAACATTCTCAGAGTT
TCTGAAGCTGAGTTTTTGGCCAGGACAGCCTGTTCCTCTGTCGAGGAGTTTCAAGTTTTTAAAAAACTCT
TGGATCAAGAGGAGGAGGAGGAGTCTGAGAGCCATGCATCTGAGGAGGAAGAGGAAGAGGAGGAAGAGGA
GGAGCTACTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR201813 protein sequence
Red=Cloning site Green=Tags(s)

MQMKRRSSYLRKLGLGEGKLKRVLSVCPEVFTMHQRDIDRVVKVLREKCLFTAQHITDVLHRCPTVLQED
PNELEYKFQYAYFRMGLTHLDIVRTNFLQYSITKIKQRHIYLERLGRYQTPDKKGQTQIPNPSLRNILRV
SEAEFLARTACSSVEEFQVFKKLLDQEEEEESESHASEEEEEEEEEEELL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC055877
ORF Size 570 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq BC055877, AAH55877
RefSeq Size 1255 bp
RefSeq ORF 572 bp
Locus ID 69821
Cytogenetics 1 D
MW 22.6 kDa
Gene Summary Regulator of mitochondrial ribosome biogenesis and translation. Binds to mitochondrial ribosomal RNAs 16S, 12S and 7S (By similarity). Targets NSUN4 RNA methyltransferase to the mitochondrial large ribosomal subunit.[UniProtKB/Swiss-Prot Function]

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