Rpl32 (NM_172086) Mouse Tagged ORF Clone

CAT#: MR200818

  • TrueORF®

Rpl32 (Myc-DDK-tagged) - Mouse ribosomal protein L32 (Rpl32)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_172086" in other vectors (3)


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Reconstitution Protocol

USD 150.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Rpl32
Synonyms AU020185; rpL32-3A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR200818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCCTCCGGCCTCTGGTGAAGCCCAAGATCGTCAAAAAGAGGACCAAGAAGTTCATCAGGCACC
AGTCAGACCGATATGTGAAAATTAAGCGAAACTGGCGGAAACCCAGAGGCATTGACAACAGGGTGCGGAG
AAGGTTCAAGGGCCAGATCCTGATGCCCAACATCGGTTATGGGAGCAACAAGAAAACCAAGCACATGCTG
CCCAGCGGCTTCCGCAAGTTCCTGGTCCACAATGTCAAGGAGCTGGAGGTGCTGCTGATGTGCAACAAAT
CTTACTGTGCTGAGATTGCTCACAATGTGTCCTCTAAGAACCGAAAAGCCATTGTAGAAAGAGCAGCACA
GCTGGCCATCAGAGTCACCAATCCCAACGCCAGGCTACGCAGCGAAGAAAATGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR200818 protein sequence
Red=Cloning site Green=Tags(s)

MAALRPLVKPKIVKKRTKKFIRHQSDRYVKIKRNWRKPRGIDNRVRRRFKGQILMPNIGYGSNKKTKHML
PSGFRKFLVHNVKELEVLLMCNKSYCAEIAHNVSSKNRKAIVERAAQLAIRVTNPNARLRSEENE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_172086
ORF Size 408 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172086.2
RefSeq Size 504 bp
RefSeq ORF 408 bp
Locus ID 19951
UniProt ID P62911
Cytogenetics 6 53.72 cM
MW 15.9 kDa

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