Fabp3 (NM_010174) Mouse Tagged ORF Clone

CAT#: MR200776

Fabp3 (Myc-DDK-tagged) - Mouse fatty acid binding protein 3, muscle and heart (Fabp3)

Product Images

Circular map for MR200776

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fabp3
• Synonyms: Fabph-1; Fabph-4; Fabph1; Fabph4; H-FABP; Mdgi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR200776 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCCTTTGTCGGTACCTGGAAGCTAGTGGACAGCAAGAATTTTGATGACTACATGAAGTCAC
  TCGGTGTGGGCTTTGCCACCAGGCAGGTGGCTAGCATGACCAAGCCTACTACCATCATCGAGAAGAACGG
  GGATACTATCACCATAAAGACACAAAGTACCTTCAAGAACACAGAGATCAACTTTCAGCTGGGAATAGAG
  TTCGACGAGGTGACAGCAGATGACCGGAAGGTCAAGTCACTGGTGACGCTGGACGGAGGCAAACTCATCC
  ATGTGCAGAAGTGGAACGGGCAGGAGACAACACTAACTAGGGAGCTAGTTGACGGGAAACTCATCCTGAC
  TCTCACTCATGGCAGTGTGGTGAGCACTCGGACTTATGAGAAGGAGGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR200776 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADAFVGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDTITIKTQSTFKNTEINFQLGIE
  FDEVTADDRKVKSLVTLDGGKLIHVQKWNGQETTLTRELVDGKLILTLTHGSVVSTRTYEKEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010174
• ORF Size: 402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010174.2
• RefSeq Size: 669 bp
• RefSeq ORF: 402 bp
• Locus ID: 14077
• UniProt ID: P11404
• Cytogenetics: 4 63.43 cM
• MW: 14.8 kDa
• Gene Summary: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.[UniProtKB/Swiss-Prot Function]