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Lamtor2 (NM_031248) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR200653L3
Lenti ORF clone of Lamtor2 (Myc-DDK-tagged) - Mouse late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 (Lamtor2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $450.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lamtor2
Synonyms 2010111E04Rik; AL022628; Mapbpip; P14; Rab25; Robld3
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR200653).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031248
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031248.3
RefSeq Size 586 bp
RefSeq ORF 378 bp
Locus ID 83409
UniProt ID Q9JHS3
Cytogenetics 3 F1
Summary As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.UniProtKB/Swiss-Prot Function
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"NM_031248" in other vectors (4)
SKU Description Size Price
MC211959 Lamtor2 (untagged) - Mouse late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 (Lamtor2), (10ug) 10 ug
$165.00
MG200653 Lamtor2 (tGFP-tagged) - Mouse mitogen activated protein binding protein interacting protein (Mapbpip) 10 ug
$350.00
MR200653 Lamtor2 (Myc-DDK-tagged) - Mouse late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 (Lamtor2) 10 ug
$289.00
MR200653L4 Lenti ORF clone of Lamtor2 (mGFP-tagged) - Mouse late endosomal/lysosomal adaptor, MAPK and MTOR activator 2 (Lamtor2) 10 ug
$450.00

Plasmid Map for MR200653L3

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Cloning scheme for MR200653L3
Circular map for MR200653L3

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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