Bmp2 (NM_007553) Mouse Tagged ORF Clone

CAT#: MG227677

Bmp2 (tGFP-tagged) - Mouse bone morphogenetic protein 2 (Bmp2), (10ug)

Product Images

Circular map for MG227677

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bmp2
• Synonyms: AI467020; Bmp; Bmp2a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227677 representing NM_007553
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCCGGGACCCGCTGTCTTCTAGTGTTGCTGCTTCCCCAGGTCCTCCTGGGCGGCGCGGCCGGCC
  TCATTCCAGAGCTGGGCCGCAAGAAGTTCGCCGCGGCATCCAGCCGACCCTTGTCCCGGCCTTCGGAAGA
  CGTCCTCAGCGAATTTGAGTTGAGGCTGCTCAGCATGTTTGGCCTGAAGCAGAGACCCACCCCCAGCAAG
  GACGTCGTGGTGCCCCCCTATATGCTAGATCTGTACCGCAGGCACTCAGGCCAGCCAGGAGCGCCCGCCC
  CAGACCACCGGCTGGAGAGGGCAGCCAGCCGCGCCAACACCGTGCGCAGCTTCCATCACGAAGAAGCCGT
  GGAGGAACTTCCAGAGATGAGTGGGAAAACGGCCCGGCGCTTCTTCTTCAATTTAAGTTCTGTCCCCAGT
  GACGAGTTTCTCACATCTGCAGAACTCCAGATCTTCCGGGAACAGATACAGGAAGCTTTGGGAAACAGTA
  GTTTCCAGCACCGAATTAATATTTATGAAATTATAAAGCCTGCAGCAGCCAACTTGAAATTTCCTGTGAC
  CAGACTATTGGACACCAGGTTAGTGAATCAGAACACAAGTCAGTGGGAGAGCTTCGACGTCACCCCAGCT
  GTGATGCGGTGGACCACACAGGGACACACCAACCATGGGTTTGTGGTGGAAGTGGCCCATTTAGAGGAGA
  ACCCAGGTGTCTCCAAGAGACATGTGAGGATTAGCAGGTCTTTGCACCAAGATGAACACAGCTGGTCACA
  GATAAGGCCATTGCTAGTGACTTTTGGACATGATGGAAAAGGACATCCGCTCCACAAACGAGAAAAGCGT
  CAAGCCAAACACAAACAGCGGAAGCGCCTCAAGTCCAGCTGCAAGAGACACCCTTTGTATGTGGACTTCA
  GTGATGTGGGGTGGAATGACTGGATCGTGGCACCTCCGGGCTATCATGCCTTTTACTGCCATGGGGAGTG
  TCCTTTTCCCCTTGCTGACCACCTGAACTCCACTAACCATGCCATAGTGCAGACTCTGGTGAACTCTGTG
  AATTCCAAAATCCCTAAGGCATGCTGTGTCCCCACAGAGCTCAGCGCAATCTCCATGTTGTACCTAGATG
  AAAATGAAAAGGTTGTGCTAAAAAATTATCAGGACATGGTTGTGGAGGGCTGCGGGTGTCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227677 representing NM_007553
  Red=Cloning site Green=Tags(s)
  
  MVAGTRCLLVLLLPQVLLGGAAGLIPELGRKKFAAASSRPLSRPSEDVLSEFELRLLSMFGLKQRPTPSK
  DVVVPPYMLDLYRRHSGQPGAPAPDHRLERAASRANTVRSFHHEEAVEELPEMSGKTARRFFFNLSSVPS
  DEFLTSAELQIFREQIQEALGNSSFQHRINIYEIIKPAAANLKFPVTRLLDTRLVNQNTSQWESFDVTPA
  VMRWTTQGHTNHGFVVEVAHLEENPGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKR
  QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSV
  NSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007553
• ORF Size: 1182 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007553.3
• RefSeq Size: 3566 bp
• RefSeq ORF: 1185 bp
• Locus ID: 12156
• UniProt ID: P21274
• Cytogenetics: 2 65.21 cM
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. While a homozygous knockout mouse for this gene exhibits embryonic lethality, conditional knockout mice have defects in bone, cartilage and heart development. [provided by RefSeq, Jul 2016]