Cd3e (NM_007648) Mouse Tagged ORF Clone

USD 300.00


Availability*
3 Weeks

Size
    • 10 ug


Product images

Specifications

Product Data
Product Name Cd3e (NM_007648) Mouse Tagged ORF Clone
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Cd3e
Synonyms AI504783; CD3; CD3epsilon; T3e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Cell Selection Neomycin
Sequence Data
>MG227669 representing NM_007648
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGTGGAACACTTTCTGGGGCATCCTGTGCCTCAGCCTCCTAGCTGTTGGCACTTGCCAGGACGATG
CCGAGAACATTGAATACAAAGTCTCCATCTCAGGAACCAGTGTAGAGTTGACGTGCCCTCTAGACAGTGA
CGAGAACTTAAAATGGGAAAAAAATGGCCAAGAGCTGCCTCAGAAGCATGATAAGCACCTGGTGCTCCAG
GATTTCTCGGAAGTCGAGGACAGTGGCTACTACGTCTGCTACACACCAGCCTCAAATAAAAACACGTACT
TGTACCTGAAAGCTCGAGTGTGTGAGTACTGTGTGGAGGTGGACCTGACAGCAGTAGCCATAATCATCAT
TGTTGACATCTGTATCACTCTGGGCTTGCTGATGGTCATTTATTACTGGAGCAAGAATAGGAAGGCCAAG
GCCAAGCCTGTGACCCGAGGAACCGGTGCTGGTAGCAGGCCCAGAGGGCAAAACAAGGAGCGGCCACCAC
CTGTTCCCAACCCAGACTATGAGCCCATCCGCAAAGGCCAGCGGGACCTGTATTCTGGCCTGAATCAGAG
AGCAGTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG227669 representing NM_007648
Red=Cloning site Green=Tags(s)

MRWNTFWGILCLSLLAVGTCQDDAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQ
DFSEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVDLTAVAIIIIVDICITLGLLMVIYYWSKNRKAK
AKPVTRGTGAGSRPRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRAV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag TurboGFP
ACCN NM_007648
ORF Size 567 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007648.4, NP_031674.1
RefSeq Size 1436 bp
RefSeq ORF 570 bp
Locus ID 12501
Cytogenetics 9 24.84 cM

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