Pdlim5 (NM_001190852) Mouse Tagged ORF Clone

CAT#: MG227623

Pdlim5 (tGFP-tagged) - Mouse PDZ and LIM domain 5 (Pdlim5) transcript variant 4, (10ug)

Product Images

Circular map for MG227623

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pdlim5
• Synonyms: 1110001A05Rik; AI987914; C87059; Enh; Enh1; Enh2; Enh3; LIM
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227623 representing NM_001190852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAACTACAGTGTGTCATTGGTCGGCCCAGCTCCTTGGGGTTTCCGGCTCCAAGGTGGCAAGGATT
  TCAACATGCCTCTGACAATCTCTAGTCTGAAGGATGGTGGCAAGGCATCTCAGGCACATGTCAGAATAGG
  GGACGTGGTTCTCAGCATCGATGGGATCAGTGCACAGGGAATGACGCATCTTGAAGCCCAGAACAAGATT
  AAGGCTTGTACGGGCTCCTTGAATATGACTCTACAAAGAGCTTCAGCTGCAGCCAAGAGTGAGCCGGTTT
  CCGTCCAGAAGCCCACAGTCACCAGCGTGTGTTCCGAGTCTGCTCAGGAGCTAGCAGAGGGACAGAGAAG
  AGGATCCCAGGGTGACATTAAGCAGCAAAATGGCCCACCAAGAAAACACATTGTGGAGCGCAACACGGAG
  TTTTATCACATACCCACTCACAGTGATGCCAGCAAGAAACGGCTGATTGAGGACACTGAGGACTGGCGCC
  CCCGGACTGGAACGACTCAGTCTCGTTCTTTCCGGATCCTTGCCCAGATCACTGGGACTGAGCATCTGAC
  AGAATCTGAAAATGACAATACGAAGAAGGCAAAGTTTTACAGTTCTCTGGAAGACCCCCTTAAGAACGGA
  CCACACCCACCTGCAGCTCCCCAGCTGTTAAAAGTTCATAGTCAAGTAGCCATAGTTTCTAAGGAAGCAG
  CCACTTACTCTAGTGTAAGCAGGTCTACAAGAACTGTAGAAGGTGCTTTGGAAGGCTTTGGAAACTTTCC
  TGCCTTCTCCCCTCCTACTAGATACAGTGCTGTAGTCGTCAGTGATGCGGCTGCCACTGTGTCTGCTGCT
  CTCGCTGCGAAAACCAGGCTCTTCGGCCCTGAAAACTCCCAGTCTCTTCTGGATGCACTGTGCATCAGCA
  CTGTCCCCAAGCCTCTAGCTCTTTCCTGTCTTCAGTCCTCTGAGGAGTCGAGTGGCTCTGTCCACGTTAA
  GAAGAGCAGTAGCACCCAGGAGCCTTCTCAGCAACCGGCGTCATCTGGAGCTTCACCTCTGAGCGCGTCT
  GAGGGCCCCGAGAGCCCAGGTTCCAGCAGGCCCTCGGTGGCTGGCCTCCGCTCCGCTGCTGCTTTCAAGC
  CTGTAGGATCCACCAGCGTCAAGTCACCTAGCTGGCAGCGTCCAAACCAAGCAGCACCTTCTACTGGGAG
  AATTTCAAACAATGCACGTTCTTCAGGTACAGGGGCGTCTGTGGGGCCTCCCCAGCCAAGTGACCAAGAC
  ACACTTGTGCAGAGAGCGGAACACATTCCAGCGGGCAAGCGGACCCCCATGTGTGCCCACTGCAACCAAG
  TTATCAGGGGACCTTTTCTTGTGGCTCTGGGGAAGTCCTGGCATCCAGAAGAATTTAACTGTGCTCATTG
  CAAAAACACAATGGCCTACATAGGCTTTGTAGAGGAGAAGGGAGCACTGTACTGTGAGCTATGCTATGAG
  AAGTTCTTTGCTCCTGAATGTGGCCGGTGCCAAAGGAAGATCCTTGGAGAAGTCATCAATGCTTTGAAGC
  AGACCTGGCATGTGTCCTGTTTTGTGTGTGTGGCCTGTGGAAAACCCATTCGTAATAATGTTTTCCACTT
  GGAAGATGGCGAGCCCTACTGTGAGACAGACTACTACGCCCTTTTTGGAACAATATGTCGTGGATGTGAA
  TTCCCCATAGAGGCTGGTGACATGTTCCTGGAAGCTCTGGGATACACTTGGCATGATACTTGCTTTGTGT
  GCTCTGTGTGTTGTGAAAGTTTGGAAGGGCAGACATTTTTCTCCAAGAAGGACAAGCCACTTTGCAAAAA
  ACATGCTCATTCTGTGAATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227623 representing NM_001190852
  Red=Cloning site Green=Tags(s)
  
  MSNYSVSLVGPAPWGFRLQGGKDFNMPLTISSLKDGGKASQAHVRIGDVVLSIDGISAQGMTHLEAQNKI
  KACTGSLNMTLQRASAAAKSEPVSVQKPTVTSVCSESAQELAEGQRRGSQGDIKQQNGPPRKHIVERNTE
  FYHIPTHSDASKKRLIEDTEDWRPRTGTTQSRSFRILAQITGTEHLTESENDNTKKAKFYSSLEDPLKNG
  PHPPAAPQLLKVHSQVAIVSKEAATYSSVSRSTRTVEGALEGFGNFPAFSPPTRYSAVVVSDAAATVSAA
  LAAKTRLFGPENSQSLLDALCISTVPKPLALSCLQSSEESSGSVHVKKSSSTQEPSQQPASSGASPLSAS
  EGPESPGSSRPSVAGLRSAAAFKPVGSTSVKSPSWQRPNQAAPSTGRISNNARSSGTGASVGPPQPSDQD
  TLVQRAEHIPAGKRTPMCAHCNQVIRGPFLVALGKSWHPEEFNCAHCKNTMAYIGFVEEKGALYCELCYE
  KFFAPECGRCQRKILGEVINALKQTWHVSCFVCVACGKPIRNNVFHLEDGEPYCETDYYALFGTICRGCE
  FPIEAGDMFLEALGYTWHDTCFVCSVCCESLEGQTFFSKKDKPLCKKHAHSVNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190852
• ORF Size: 1842 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190852.1, NP_001177781.1
• RefSeq Size: 5108 bp
• RefSeq ORF: 1845 bp
• Locus ID: 56376
• UniProt ID: Q8CI51
• Cytogenetics: 3 H1
• Gene Summary: May play an important role in the heart development by scaffolding PKC to the Z-disk region. Isoform 2 and isoform 3 may negatively modulate the scaffolding activity of isoform 1. May play a role in the regulation of cardiomyocyte expansion. Overexpression promotes the development of heart hypertrophy. Contributes to the regulation of dendritic spine morphogenesis in neurons. May restrain postsynaptic growth of excitatory synapses (By similarity).[UniProtKB/Swiss-Prot Function]