Nt5e (NM_011851) Mouse Tagged ORF Clone

CAT#: MG227439

Nt5e (tGFP-tagged) - Mouse 5' nucleotidase ecto (Nt5e), (10ug)

Product Images

Circular map for MG227439

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nt5e
• Synonyms: 5'-NT; 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227439 representing NM_011851
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTCCCGCGGCCGCTAAGGTACCCAAGTGGCTGCTTCTCGCACTGAGCGCTCTACTACCACAGTGGC
  CCGCAGCCAGTGCCTGGGAGCTCACGATCCTGCACACAAACGACGTGCACAGCCGGCTAGAGCAGACCAG
  CGATGACTCCACCAAGTGCCTCAACGCCAGCCTGTGTGTGGGCGGCGTGGCCCGGCTCTTTACCAAGGTG
  CAGCAGATCCGCAAGGAAGAACCCAACGTGCTGTTTTTGGATGCCGGAGACCAGTACCAGGGCACCATCT
  GGTTCACCGTTTACAAAGGCCTTGAAGTGGCACACTTCATGAACATCCTGGGCTACGATGCTATGGCACT
  GGGAAATCATGAATTTGATAACGGTGTGGAAGGACTGATTGATCCCCTCCTCAGAAACGTTAAATTTCCA
  ATTCTGAGCGCAAACATTAAGGCACGGGGGCCTCTAGCACATCAGATATCTGGACTTTTTCTGCCATCTA
  AAGTTCTCTCTGTTGGCGGTGAGGTTGTGGGGATTGTTGGATATACTTCAAAGGAAACCCCTTTCCTCTC
  AAATCCAGGGACAAATTTAGTCTTTGAAGATGAAATCTCTGCATTGCAGCCTGAAGTAGATAAACTAAAG
  ACGCTAAATGTGAATAAGATCATCGCCCTGGGGCACTCTGGTTTTGAGATGGACAAACTTATCGCTCAGA
  AAGTTCGAGGTGTGGACATCGTGGTGGGAGGACACTCCAACACCTTTCTCTACACAGGAAATCCACCTTC
  CAAAGAAGTGCCTGCGGGGAAGTACCCATTCATAGTCACCGCAGATGATGGACGGCAGGTGCCTGTGGTC
  CAGGCCTATGCCTTTGGCAAATACCTGGGCTACCTGAAGGTTGAGTTTGATGATAAAGGCAATGTTATCA
  CTTCCTATGGAAATCCCATTCTTCTCAACAGCAGCATTCCTGAAGATGCGACCATCAAAGCAGACATTAA
  CCAATGGAGGATAAAATTAGATAATTATTCTACCCAGGAACTCGGGAGAACGATCGTCTACCTGGATGGC
  TCCACTCAGACGTGCCGCTTCAGGGAATGCAACATGGGAAACCTGATCTGTGATGCCATGATTAACAACA
  ACCTCAGACACCCAGATGAAATGTTTTGGAACCACGTGTCCATGTGCATTGTAAACGGAGGTGGCATCCG
  GTCCCCCATTGATGAGAAGAACAATGGTACCATCACCTGGGAGAACCTGGCTGCTGTGCTGCCCTTTGGA
  GGGACATTTGACCTCGTCCAATTAAAAGGGTCCACCCTGAAGAAGGCTTTTGAGCACAGCGTGCATCGCT
  ATGGCCAGTCCACCGGAGAGTTCCTGCAAGTGGGTGGAATCCATGTGGTGTACGATATTAACCGAAAGCC
  CTGGAACAGAGTGGTCCAATTAGAAGTTCTCTGCACCAAGTGTCGAGTGCCCATCTATGAGCCTCTTGAA
  ATGGATAAAGTGTATAAAGTGACCCTCCCAAGCTATCTGGCCAACGGTGGAGATGGATTCCAGATGATAA
  AAGATGAATTACTAAAGCATGACTCTGGTGATCAAGATATCAGCGTGGTTTCTGAATACATCTCAAAAAT
  GAAAGTAGTTTACCCAGCCGTTGAAGGGCGGATCAAGTTCTCTGCAGCAAGTCATTACCAGGGAAGCTTT
  CCTTTAGTAATTCTTTCATTTTGGGCAATGATCCTTATTTTGTACCAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227439 representing NM_011851
  Red=Cloning site Green=Tags(s)
  
  MRPAAAKVPKWLLLALSALLPQWPAASAWELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKV
  QQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFP
  ILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLK
  TLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVV
  QAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDG
  STQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFG
  GTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLE
  MDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFSAASHYQGSF
  PLVILSFWAMILILYQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011851
• ORF Size: 1728 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011851.4, NP_035981.1
• RefSeq Size: 3580 bp
• RefSeq ORF: 1731 bp
• Locus ID: 23959
• UniProt ID: Q61503
• Cytogenetics: 9 E3.1
• Gene Summary: This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]