Grm5 (NM_001143834) Mouse Tagged ORF Clone

CAT#: MG227391

Grm5 (tGFP-tagged) - Mouse glutamate receptor metabotropic 5 (Grm5) transcript variant b, (10ug)

Product Images

Circular map for MG227391

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grm5
• Synonyms: 6430542K11Rik; AI850523; Glu5R; Gprc1e; mGluR5; mGluR5b
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227391 representing NM_001143834
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCTTCTGTTGATTCTGTCAGTCCTACTTCTGAAAGAAGATGTACGAGGAAGTGCACAGTCCAGTG
  AGAGGAGGGTGGTGGCTCACATGCCAGGTGACATTATTATTGGAGCTCTCTTCTCGGTCCACCACCAACC
  AACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCAGTCCGTGAGCAGTATGGCATTCAGAGAGTGGAA
  GCCATGCTGCATACCTTGGAAAGGATCAATTCAGATCCCACACTCTTGCCCAACATCACACTAGGCTGTG
  AGATAAGAGATTCCTGCTGGCATTCTGCTGTGGCCCTAGAGCAAAGCATTGAGTTTATAAGGGATTCCCT
  CATCTCTTCGGAAGAGGAAGAAGGCTTGGTACGCTGTGTAGATGGCTCTTCTTCCTTCCGCTCCAAGAAA
  CCCATAGTGGGAGTCATTGGGCCTGGCTCGAGTTCTGTGGCCATTCAAGTTCAGAACTTGCTCCAGCTTT
  TCAACATACCTCAGATTGCTTACTCTGCAACTAGCATGGATTTGAGTGACAAGACTCTATTCAAGTACTT
  CATGAGGGTTGTACCTTCGGATGCCCAGCAAGCCCGAGCCATGGTAGACATAGTGAAGAGATACAACTGG
  ACTTATGTCTCAGCTGTGCACACAGAAGGCAACTATGGAGAAAGTGGGATGGAGGCTTTCAAAGATATGT
  CAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAAATCTACAGCAATGCTGGGGAACAGAGCTTTGA
  CAAGCTGTTGAAAAAGCTCAGAAGTCATTTACCTAAAGCCCGGGTGGTAGCCTGCTTCTGTGAAGGCATG
  ACAGTTCGAGGTCTGCTCATGGCCATGAGACGCTTGGGTCTAGCAGGGGAATTTCTACTTCTGGGCAGTG
  ATGGCTGGGCTGACAGGTATGACGTGACAGATGGGTATCAGCGAGAAGCTGTCGGTGGGATTACAATCAA
  GCTCCAGTCTCCTGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCAGAAACAAACCTCAGA
  AACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGGCTAGAAGGGTTTGCACAGGAGAACA
  GCAAGTACAACAAGACTTGCAACAGTTCTCTAACTCTGAGAACGCATCATGTTCAAGATTCCAAAATGGG
  ATTTGTGATCAATGCAATCTATTCTATGGCTTATGGGCTCCACAACATGCAGATGTCCCTGTGTCCAGGC
  TATGCAGGCCTCTGTGATGCAATGAAGCCAATTGATGGGCGGAAACTTTTGGACTCCCTGATGAAAACCA
  ACTTTACTGGAGTTTCCGGAGATATGATTCTATTTGATGAAAATGGAGACTCTCCAGGAAGGTATGAAAT
  AATGAATTTCAAGGAAATGGGAAAAGATTATTTTGATTACATCAATGTTGGAAGTTGGGACAATGGGGAA
  TTAAAAATGGATGATGATGAAGTGTGGTCCAAGAAAAATAACATCATCAGATCTGTGTGCAGTGAACCGT
  GTGAGAAAGGACAGATAAAGGTGATCCGGAAGGGAGAAGTCAGCTGTTGTTGGACCTGCACACCTTGTAA
  GGAGAATGAGTATGTTTTTGATGAGTACACCTGCAAGGCGTGCCAACTGGGGTCCTGGCCCACTGACGAC
  TTGACAGGTTGTGATTTGATCCCAGTCCAGTATCTTCGATGGGGTGACCCTGAGCCCATTGCAGCTGTGG
  TGTTTGCCTGCCTCGGTCTGCTAGCCACCCTCTTCGTTACTGTAATCTTCATCATTTATCGGGACACTCC
  AGTGGTCAAGTCTTCCAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGCCTGGGTTACTTGTGT
  ACCTTCTGCCTCATTGCAAAGCCCAAACAGATTTACTGCTACCTTCAGAGAATTGGCATCGGTCTCTCTC
  CAGCCATGAGCTACTCAGCCCTTGTAACGAAGACCAACCGTATTGCAAGGATTCTAGCTGGCAGCAAGAA
  GAAGATCTGTACCAAAAAGCCCAGATTCATGAGCGCCTGTGCTCAGTTAGTGATCGCTTTCATTCTCATC
  TGTATACAGTTGGGTATTATTGTGGCCCTCTTTATCATGGAGCCTCCGGATATAATGCATGACTATCCAA
  GCATCCGAGAAGTCTACTTGATTTGTAACACCACCAACCTAGGGGTTGTCACTCCTCTTGGATACAATGG
  ATTATTGATTTTGAGTTGCACATTCTATGCGTTCAAGACCAGAAATGTTCCAGCCAACTTTAACGAGGCC
  AAATATATTGCTTTCACCATGTACACAACCTGCATCATATGGCTGGCCTTTGTGCCTATCTACTTTGGCA
  GCAACTACAAAATCATCACCATGTGTTTCTCAGTCAGCCTCAGTGCCACAGTGGCCCTGGGTTGCATGTT
  TGTGCCGAAGGTGTACATCATCCTAGCCAAACCGGAGAGAAATGTGCGCAGCGCCTTCACAACCTCTACA
  GTGGTGCGCATGCACGTAGGAGATGGCAAGTCATCATCCGCTGCCAGCAGATCCAGCAGCCTAGTCAACC
  TGTGGAAGAGGAGGGGCTCGTCTGGGGAAACCCTAAGGTACAAAGACAGGAGACTGGCCCAGCACAAGTC
  GGAAATAGAGTGTTTCACCCCCAAAGGGAGTATGGGGAATGGTGGGAGAGCAACAATGAGCAGCTCCAAT
  GGAAAATCCGTGACTTGGGCCCAGAATGAGAAGAGCACCCGGGGGCAGCACCTGTGGCAGCGACTGTCTG
  TCCATATCAACAAGAAGGAGAACCCCAACCAGACAGCAGTCATCAAACCCTTCCCCAAGAGCACAGAGAG
  CCGCGGGCAGGGTGCAGGGGCAGGTGGTGGCTCTGGCCCCGGTGCAGCTGGTGCTGGTAGCGCAGGATGC
  ACAGCGACAGGCGGCCCAGAGCCACCAGACGCCGGCCCCAAGGCGCTTTATGATGTCGCAGAGGCAGAGG
  AGCGCTTCCCAGCGGCTGCCAGGCCGCGCTCGCCATCGCCCATCAGTACGCTGAGCCACCTGGCAGGCTC
  GGCGGGCCGCACAGACGACGACGCGCCGTCGCTGCACTCGGAGACCGCTGCACGCAGCAGCTCATCCCAG
  GGCTCGCTCATGGAGCAGATTAGCAGCGTGGTGACGCGCTTCACCGCCAACATCACCGAGCTCAACTCCA
  TGATGTTGTCCACCGCGGCTGCGCCGGGGCCCCCTGGTACCCCTATCTGCTCTTCCTACCTGATCCCCAA
  AGAGATCCAGCTGCCCACGACCATGACGACCTTCGCAGAGATCCAGCCTCTGCCGGCCATCGAGGTGACC
  GGAGGAGCTCAGCCGGCGACAGGGCCATCACCTGCCCAAGAGACGCCCGCAGGAGCTGAAGCCGCCCCAG
  GAAAACCGGATCTGGAGGAGCTGGTGGCCCTCACTCCACCATCGCCCTTCAGGGACTCGGTGGACTCGGG
  GAGCACCACCCCAAACTCTCCAGTCTCCGAATCGGCCCTCTGCATCCCATCCTCTCCCAAATATGACACT
  CTCATCATCAGAGATTACACGCAGAGTTCTTCATCGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227391 representing NM_001143834
  Red=Cloning site Green=Tags(s)
  
  MVLLLILSVLLLKEDVRGSAQSSERRVVAHMPGDIIIGALFSVHHQPTVDKVHERKCGAVREQYGIQRVE
  AMLHTLERINSDPTLLPNITLGCEIRDSCWHSAVALEQSIEFIRDSLISSEEEEGLVRCVDGSSSFRSKK
  PIVGVIGPGSSSVAIQVQNLLQLFNIPQIAYSATSMDLSDKTLFKYFMRVVPSDAQQARAMVDIVKRYNW
  TYVSAVHTEGNYGESGMEAFKDMSAKEGICIAHSYKIYSNAGEQSFDKLLKKLRSHLPKARVVACFCEGM
  TVRGLLMAMRRLGLAGEFLLLGSDGWADRYDVTDGYQREAVGGITIKLQSPDVKWFDDYYLKLRPETNLR
  NPWFQEFWQHRFQCRLEGFAQENSKYNKTCNSSLTLRTHHVQDSKMGFVINAIYSMAYGLHNMQMSLCPG
  YAGLCDAMKPIDGRKLLDSLMKTNFTGVSGDMILFDENGDSPGRYEIMNFKEMGKDYFDYINVGSWDNGE
  LKMDDDEVWSKKNNIIRSVCSEPCEKGQIKVIRKGEVSCCWTCTPCKENEYVFDEYTCKACQLGSWPTDD
  LTGCDLIPVQYLRWGDPEPIAAVVFACLGLLATLFVTVIFIIYRDTPVVKSSSRELCYIILAGICLGYLC
  TFCLIAKPKQIYCYLQRIGIGLSPAMSYSALVTKTNRIARILAGSKKKICTKKPRFMSACAQLVIAFILI
  CIQLGIIVALFIMEPPDIMHDYPSIREVYLICNTTNLGVVTPLGYNGLLILSCTFYAFKTRNVPANFNEA
  KYIAFTMYTTCIIWLAFVPIYFGSNYKIITMCFSVSLSATVALGCMFVPKVYIILAKPERNVRSAFTTST
  VVRMHVGDGKSSSAASRSSSLVNLWKRRGSSGETLRYKDRRLAQHKSEIECFTPKGSMGNGGRATMSSSN
  GKSVTWAQNEKSTRGQHLWQRLSVHINKKENPNQTAVIKPFPKSTESRGQGAGAGGGSGPGAAGAGSAGC
  TATGGPEPPDAGPKALYDVAEAEERFPAAARPRSPSPISTLSHLAGSAGRTDDDAPSLHSETAARSSSSQ
  GSLMEQISSVVTRFTANITELNSMMLSTAAAPGPPGTPICSSYLIPKEIQLPTTMTTFAEIQPLPAIEVT
  GGAQPATGPSPAQETPAGAEAAPGKPDLEELVALTPPSPFRDSVDSGSTTPNSPVSESALCIPSSPKYDT
  LIIRDYTQSSSSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143834
• ORF Size: 3609 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001143834.1, NP_001137306.1
• RefSeq Size: 8524 bp
• RefSeq ORF: 3612 bp
• Locus ID: 108071
• UniProt ID: Q3UVX5
• Cytogenetics: 7 D3
• Gene Summary: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity (By similarity).[UniProtKB/Swiss-Prot Function]