Crebbp (NM_001025432) Mouse Tagged ORF Clone

CAT#: MG227381

Crebbp (tGFP-tagged) - Mouse CREB binding protein (Crebbp), (10ug)

Product Images

Circular map for MG227381

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Crebbp
• Synonyms: AW558298; CBP; CBP/p300; KAT3A; p300/CBP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227381 representing NM_001025432
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGAACTTGCTGGACGGACCGCCCAACCCCAAACGAGCCAAACTCAGCTCGCCCGGCTTCTCCG
  CGAATGACAACACAGATTTTGGATCATTGTTTGACTTGGAAAATGACCTTCCTGATGAGCTGATCCCCAA
  TGGAGAATTAAGCCTTTTAAACAGTGGGAACCTTGTTCCAGATGCTGCGTCCAAACATAAACAACTGTCA
  GAGCTTCTTAGAGGAGGCAGCGGCTCTAGCATCAACCCAGGGATAGGCAATGTGAGTGCCAGCAGCCCTG
  TGCAACAGGGCCTTGGTGGCCAGGCTCAGGGGCAGCCGAACAGTACAAACATGGCCAGCTTAGGTGCCAT
  GGGCAAGAGCCCTCTGAACCAAGGAGACTCATCAACACCCAACCTGCCCAAACAGGCAGCCAGCACCTCT
  GGGCCCACTCCCCCTGCCTCCCAAGCACTGAATCCACAAGCACAAAAGCAAGTAGGGCTGGTGACCAGTA
  GTCCTGCCACATCACAGACTGGACCTGGGATCTGCATGAATGCTAACTTCAACCAGACCCACCCAGGCCT
  TCTCAATAGTAACTCTGGCCATAGCTTAATGAATCAGGCTCAACAAGGGCAAGCTCAAGTCATGAATGGA
  TCTCTTGGGGCTGCTGGAAGAGGAAGGGGAGCTGGAATGCCCTACCCTGCTCCAGCCATGCAGGGGGCCA
  CAAGCAGTGTGCTGGCGGAGACCTTGACACAGGTTTCCCCACAAATGGCTGGCCATGCTGGACTAAATAC
  AGCACAGGCAGGAGGCATGACCAAGATGGGAATGACTGGTACCACAAGTCCATTTGGACAACCCTTTAGT
  CAAACTGGAGGGCAGCAGATGGGAGCCACTGGAGTGAACCCCCAGTTAGCCAGCAAACAGAGCATGGTCA
  ATAGTTTACCTGCTTTTCCTACAGATATCAAGAATACTTCAGTCACCACTGTGCCAAATATGTCCCAGTT
  GCAAACATCAGTGGGAATTGTACCCACACAAGCAATTGCAACAGGCCCCACAGCAGACCCTGAAAAACGC
  AAACTGATACAGCAGCAGCTGGTTCTACTGCTTCATGCCCACAAATGTCAGAGACGAGAGCAAGCAAATG
  GAGAGGTTCGAGCCTGTTCTCTCCCACACTGTCGAACCATGAAAAACGTTTTGAATCACATGACACATTG
  TCAGGCTGGGAAAGCCTGCCAAGTTGCCCATTGTGCATCTTCACGACAAATCATCTCTCATTGGAAGAAC
  TGCACACGACATGACTGTCCTGTTTGCCTCCCTTTGAAAAATGCCAGTGACAAGCGAAACCAACAAACCA
  TCCTGGGATCTCCAGCTAGTGGAATTCAAAACACAATTGGTTCTGTTGGTGCAGGGCAACAGAATGCCAC
  TTCCTTAAGTAACCCAAATCCCATAGACCCCAGTTCCATGCAGCGGGCCTATGCTGCTCTAGGACTCCCC
  TACATGAACCAGCCTCAGACGCAGCTGCAGCCTCAGGTTCCTGGCCAGCAACCAGCACAGCCTCCAGCCC
  ACCAGCAGATGAGGACTCTCAATGCCCTAGGAAACAACCCCATGAGTATCCCAGCAGGAGGAATAACAAC
  AGATCAACAGCCACCAAACTTGATTTCAGAATCAGCTCTTCCAACTTCCTTGGGGGCTACCAATCCACTG
  ATGAATGATGGTTCAAACTCTGGTAACATTGGAAGCCTCAGCACGATACCTACAGCAGCGCCTCCTTCCA
  GCACTGGTGTTCGAAAAGGCTGGCATGAACATGTGACTCAGGACCTACGGAGTCATCTAGTCCATAAACT
  CGTTCAAGCCATCTTCCCAACTCCAGACCCTGCAGCTCTGAAAGATCGCCGCATGGAGAACCTGGTTGCC
  TATGCTAAGAAAGTGGAGGGAGACATGTATGAGTCTGCTAATAGCAGGGATGAATACTATCATTTATTAG
  CAGAGAAAATCTATAAAATACAAAAAGAACTAGAAGAAAAGCGGAGGTCACGTTTACATAAGCAAGGCAT
  CCTGGGTAACCAGCCAGCTTTACCAGCTTCTGGGGCTCAGCCCCCTGTGATTCCACCAGCCCAGTCTGTA
  AGACCTCCAAATGGGCCCCTGCCTTTGCCAGTGAATCGCATGCAGGTTTCTCAAGGGATGAATTCATTTA
  ACCCAATGTCCCTGGGAAACGTCCAGTTGCCACAGGCACCCATGGGACCTCGTGCAGCCTCCCCTATGAA
  CCACTCTGTGCAGATGAACAGCATGGCCTCAGTTCCGGGTATGGCCATTTCTCCTTCACGGATGCCTCAG
  CCTCCAAATATGATGGGCACTCATGCCAACAACATTATGGCCCAGGCACCTACTCAGAACCAGTTTCTGC
  CACAGAACCAGTTTCCATCATCCAGTGGGGCAATGAGTGTGAACAGTGTGGGCATGGGGCAACCAGCAGC
  CCAGGCAGGTGTTTCACAGGGTCAGGTACCTGGAGCTGCTCTCCCTAACCCTCTGAACATGCTGGCACCC
  CAGGCCAGCCAGCTGCCTTGCCCACCAGTGACACAGTCACCATTGCACCCGACTCCACCTCCTGCTTCCA
  CAGCTGCTGGCATGCCCTCTCTCCAACATCCAACGGCACCAGGAATGACCCCTCCTCAGCCAGCAGCTCC
  CACTCAGCCATCTACTCCTGTGTCATCTGGGCAGACTCCTACCCCAACTCCTGGCTCAGTGCCCAGCGCT
  GCCCAAACACAGAGTACCCCTACAGTCCAGGCAGCAGCACAGGCTCAGGTGACTCCACAGCCTCAGACCC
  CAGTGCAGCCACCATCTGTGGCTACTCCTCAGTCATCACAGCAGCAACCAACGCCTGTGCATACTCAGCC
  TCCTGGCACACCGCTTTCTCAGGCAGCAGCCAGCATTGATAATAGAGTCCCTACTCCCTCCTCTGTGACC
  AGTGCTGAAACCAGTTCCCAGCAGCCAGGACCCGATGTGCCCATGCTGGAAATGAAGACAGAGGTGCAGA
  CAGATGATGCTGAGCCTGAACCTACTGAATCCAAGGGGGAACCTCGGTCTGAGATGATGGAAGAGGATTT
  ACAAGGTTCTTCCCAAGTAAAAGAAGAGACAGATACGACAGAGCAGAAGTCAGAGCCAATGGAAGTAGAA
  GAAAAGAAACCTGAAGTAAAAGTGGAAGCTAAAGAGGAAGAAGAGAACAGTTCGAACGACACAGCCTCAC
  AATCAACATCTCCTTCCCAGCCACGCAAAAAAATCTTTAAACCCGAGGAGCTACGCCAGGCACTTATGCC
  AACTCTAGAAGCACTCTATCGACAGGACCCAGAGTCTTTGCCTTTTCGTCAGCCTGTAGATCCTCAGCTC
  CTAGGAATCCCAGATTATTTTGATATAGTGAAGAATCCTATGGACCTTTCTACCATCAAACGAAAGCTGG
  ACACAGGGCAATATCAAGAACCCTGGCAGTATGTGGATGATGTCTGGCTTATGTTCAACAATGCGTGGCT
  ATATAATCGTAAAACGTCCCGTGTATATAAATTTTGCAGTAAACTTGCAGAGGTCTTTGAACAAGAAATT
  GACCCTGTCATGCAGTCTCTTGGATATTGCTGTGGACGAAAGTATGAGTTCTCCCCACAGACTTTGTGCT
  GTTACGGAAAGCAGCTGTGTACAATTCCTCGTGATGCAGCCTACTACAGCTATCAGAATAGGTATCATTT
  CTGTGAGAAGTGTTTCACAGAGATCCAGGGCGAGAATGTGACCCTGGGTGACGACCCTTCCCAACCTCAG
  ACGACAATTTCCAAGGATCAATTTGAAAAGAAGAAAAATGATACCTTAGATCCTGAACCTTTTGTTGACT
  GCAAAGAGTGTGGCCGGAAGATGCATCAGATTTGTGTTCTACACTATGACATCATTTGGCCTTCAGGTTT
  TGTGTGTGACAACTGTTTGAAGAAAACTGGCAGACCTCGGAAAGAAAACAAATTCAGTGCTAAGAGGCTG
  CAGACCACACGATTGGGAAACCACTTAGAAGACAGAGTGAATAAGTTTTTGCGGCGCCAGAATCACCCTG
  AAGCTGGGGAGGTTTTTGTCAGAGTGGTGGCCAGCTCAGACAAGACTGTGGAGGTCAAGCCGGGAATGAA
  GTCAAGGTTTGTGGATTCTGGAGAGATGTCGGAATCTTTCCCATATCGTACCAAAGCACTCTTTGCTTTT
  GAGGAGATCGATGGAGTCGATGTGTGCTTTTTTGGGATGCATGTGCAAGAATACGGCTCTGATTGCCCCC
  CACCAAATACAAGGCGTGTATACATATCTTATCTGGACAGTATTCATTTCTTCCGGCCCCGCTGCCTCCG
  GACAGCTGTTTACCATGAGATCCTCATCGGATATCTCGAGTATGTGAAGAAATTGGGGTATGTGACAGGA
  CATATTTGGGCCTGTCCCCCAAGTGAAGGAGATGACTATATCTTTCATTGCCACCCCCCTGACCAGAAAA
  TCCCCAAACCAAAACGACTACAGGAGTGGTACAAGAAGATGCTGGACAAGGCGTTTGCAGAGAGGATCAT
  TAACGACTATAAGGACATCTTCAAACAAGCGAACGAAGACAGGCTCACGAGTGCCAAGGAGTTGCCCTAT
  TTTGAAGGAGATTTCTGGCCTAATGTGTTGGAAGAAAGCATTAAGGAACTAGAACAAGAAGAAGAAGAAA
  GGAAAAAAGAAGAGAGTACTGCAGCGAGTGAGACTCCTGAGGGCAGTCAGGGTGACAGCAAAAATGCGAA
  GAAAAAGAACAACAAGAAGACCAACAAAAACAAAAGCAGCATTAGCCGCGCCAACAAGAAGAAGCCCAGC
  ATGCCCAATGTTTCCAACGACCTGTCGCAGAAGCTGTATGCCACCATGGAGAAGCACAAGGAGGTATTCT
  TTGTGATTCATCTGCATGCTGGGCCTGTTATCAGCACTCAGCCCCCCATCGTGGACCCTGATCCTCTGCT
  TAGCTGTGACCTCATGGATGGGCGAGATGCCTTCCTCACCCTGGCCAGAGACAAGCACTGGGAATTCTCT
  TCCTTACGCCGCTCCAAATGGTCCACTCTGTGCATGCTGGTGGAGCTGCACACACAGGGCCAGGACCGCT
  TTGTTTATACCTGCAATGAGTGCAAACACCATGTGGAAACACGCTGGCACTGCACTGTGTGTGAGGACTA
  TGACCTTTGTATCAATTGCTACAACACAAAGAGCCACACCCATAAGATGGTGAAGTGGGGGCTAGGCCTA
  GATGATGAGGGCAGCAGTCAGGGTGAGCCACAGTCCAAGAGCCCCCAGGAATCCCGGCGTCTCAGCATCC
  AGCGCTGCATCCAGTCCCTGGTGCATGCCTGCCAGTGTCGCAATGCCAACTGCTCACTGCCGTCTTGCCA
  GAAGATGAAGCGAGTCGTGCAGCACACCAAGGGCTGCAAGCGCAAGACTAATGGAGGATGCCCAGTGTGC
  AAGCAGCTCATTGCTCTTTGCTGCTACCACGCCAAACACTGCCAAGAAAATAAATGCCCTGTGCCCTTCT
  GCCTCAACATCAAACATAAGCTCCGCCAGCAGCAGATCCAGCATCGCCTGCAGCAGGCTCAGCTCATGCG
  CCGGCGAATGGCAACCATGAACACCCGCAATGTGCCTCAGCAGAGTTTGCCTTCTCCTACCTCAGCACCA
  CCCGGGACTCCTACACAGCAGCCCAGCACACCCCAAACACCACAGCCCCCAGCCCAGCCTCAGCCTTCAC
  CTGTTAACATGTCACCAGCTGGCTTCCCTAATGTAGCCCGGACTCAGCCCCCAACAATAGTGTCTGCTGG
  GAAGCCTACCAACCAGGTGCCAGCTCCCCCACCCCCTGCCCAGCCCCCACCTGCAGCAGTAGAAGCAGCC
  CGGCAAATTGAACGTGAGGCCCAGCAGCAGCAGCACCTATACCGAGCAAACATCAACAATGGCATGCCCC
  CAGGACGTGCAGGTATGGGGACCCCAGGAAGCCAAATGACTCCTGTGGGCCTGAATGTGCCCCGTCCCAA
  CCAAGTCAGTGGGCCTGTCATGTCTAGTATGCCACCTGGGCAGTGGCAGCAGGCACCCATCCCTCAGCAG
  CAGCCGATGCCAGGCATGCCCAGGCCTGTAATGTCCATGCAGGCCCAGGCAGCAGTGGCTGGGCCACGGA
  TGCCCAATGTGCAGCCACCAAGGAGCATCTCGCCAAGTGCCCTGCAAGACCTGCTACGGACCCTAAAGTC
  ACCCAGCTCTCCTCAGCAGCAGCAGCAGGTGCTGAACATCCTTAAATCAAACCCACAGCTAATGGCAGCT
  TTCATCAAACAGCGCACAGCCAAGTATGTGGCCAATCAGCCTGGCATGCAGCCCCAGCCCGGACTTCAAT
  CCCAGCCTGGTATGCAGCCCCAGCCTGGCATGCACCAGCAGCCTAGTTTGCAAAACCTGAACGCAATGCA
  AGCTGGTGTGCCACGGCCTGGTGTGCCTCCACCACAACCAGCAATGGGAGGCCTGAATCCCCAGGGACAA
  GCTCTGAACATCATGAACCCAGGACACAACCCCAACATGACAAACATGAATCCACAGTACCGAGAAATGG
  TGAGGAGACAGCTGCTACAGCACCAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAACAAAA
  TAGTGCCAGCTTGGCCGGGGGCATGGCGGGACACAGCCAGTTCCAGCAGCCACAAGGACCTGGAGGTTAT
  GCCCCAGCCATGCAGCAGCAACGCATGCAACAGCACCTCCCCATCCAGGGCAGCTCCATGGGCCAGATGG
  CTGCTCCAATGGGACAACTTGGCCAGATGGGGCAGCCTGGGCTAGGGGCAGACAGCACCCCTAATATCCA
  GCAGGCCCTGCAGCAACGGATTCTGCAGCAGCAGCAGATGAAGCAACAAATTGGGTCACCAGGCCAGCCG
  AACCCCATGAGCCCCCAGCAGCACATGCTCTCAGGACAGCCACAGGCCTCACATCTCCCTGGCCAGCAGA
  TCGCCACATCCCTTAGTAACCAGGTGCGATCTCCAGCCCCTGTGCAGTCTCCACGGCCCCAATCCCAACC
  TCCACATTCCAGCCCGTCACCACGGATACAACCCCAGCCTTCACCACACCATGTTTCACCCCAGACTGGT
  TCCCCTCACCCTGGACTCGCAGTCACCATGGCCAGCTCCATGGATCAGGGACACCTGGGGAACCCTGAAC
  AGAGTGCAATGCTCCCCCAGCTGAATACCCCCAACAGGAGCGCACTGTCCAGTGAACTGTCCCTGGTTGG
  TGATACCACGGGAGACACACTAGAAAAGTTTGTGGAGGGTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227381 representing NM_001025432
  Red=Cloning site Green=Tags(s)
  
  MAENLLDGPPNPKRAKLSSPGFSANDNTDFGSLFDLENDLPDELIPNGELSLLNSGNLVPDAASKHKQLS
  ELLRGGSGSSINPGIGNVSASSPVQQGLGGQAQGQPNSTNMASLGAMGKSPLNQGDSSTPNLPKQAASTS
  GPTPPASQALNPQAQKQVGLVTSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLMNQAQQGQAQVMNG
  SLGAAGRGRGAGMPYPAPAMQGATSSVLAETLTQVSPQMAGHAGLNTAQAGGMTKMGMTGTTSPFGQPFS
  QTGGQQMGATGVNPQLASKQSMVNSLPAFPTDIKNTSVTTVPNMSQLQTSVGIVPTQAIATGPTADPEKR
  KLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKN
  CTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGAGQQNATSLSNPNPIDPSSMQRAYAALGLP
  YMNQPQTQLQPQVPGQQPAQPPAHQQMRTLNALGNNPMSIPAGGITTDQQPPNLISESALPTSLGATNPL
  MNDGSNSGNIGSLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVA
  YAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPASGAQPPVIPPAQSV
  RPPNGPLPLPVNRMQVSQGMNSFNPMSLGNVQLPQAPMGPRAASPMNHSVQMNSMASVPGMAISPSRMPQ
  PPNMMGTHANNIMAQAPTQNQFLPQNQFPSSSGAMSVNSVGMGQPAAQAGVSQGQVPGAALPNPLNMLAP
  QASQLPCPPVTQSPLHPTPPPASTAAGMPSLQHPTAPGMTPPQPAAPTQPSTPVSSGQTPTPTPGSVPSA
  AQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHTQPPGTPLSQAAASIDNRVPTPSSVT
  SAETSSQQPGPDVPMLEMKTEVQTDDAEPEPTESKGEPRSEMMEEDLQGSSQVKEETDTTEQKSEPMEVE
  EKKPEVKVEAKEEEENSSNDTASQSTSPSQPRKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQL
  LGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEI
  DPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNRYHFCEKCFTEIQGENVTLGDDPSQPQ
  TTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRPRKENKFSAKRL
  QTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAF
  EEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTG
  HIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIINDYKDIFKQANEDRLTSAKELPY
  FEGDFWPNVLEESIKELEQEEEERKKEESTAASETPEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPS
  MPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVISTQPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFS
  SLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHTHKMVKWGLGL
  DDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVC
  KQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAP
  PGTPTQQPSTPQTPQPPAQPQPSPVNMSPAGFPNVARTQPPTIVSAGKPTNQVPAPPPPAQPPPAAVEAA
  RQIEREAQQQQHLYRANINNGMPPGRAGMGTPGSQMTPVGLNVPRPNQVSGPVMSSMPPGQWQQAPIPQQ
  QPMPGMPRPVMSMQAQAAVAGPRMPNVQPPRSISPSALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAA
  FIKQRTAKYVANQPGMQPQPGLQSQPGMQPQPGMHQQPSLQNLNAMQAGVPRPGVPPPQPAMGGLNPQGQ
  ALNIMNPGHNPNMTNMNPQYREMVRRQLLQHQQQQQQQQQQQQQQQNSASLAGGMAGHSQFQQPQGPGGY
  APAMQQQRMQQHLPIQGSSMGQMAAPMGQLGQMGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQP
  NPMSPQQHMLSGQPQASHLPGQQIATSLSNQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTG
  SPHPGLAVTMASSMDQGHLGNPEQSAMLPQLNTPNRSALSSELSLVGDTTGDTLEKFVEGL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001025432
• ORF Size: 7323 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001025432.1, NP_001020603.1
• RefSeq Size: 7507 bp
• RefSeq ORF: 7326 bp
• Locus ID: 12914
• Cytogenetics: 16 2.4 cM