Hspa1a (NM_010479) Mouse Tagged ORF Clone

CAT#: MG227368

Hspa1a (tGFP-tagged) - Mouse heat shock protein 1A (Hspa1a), (10ug)

Product Images

Circular map for MG227368

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hspa1a
• Synonyms: hsp68; Hsp70-3; Hsp70.3; hsp70A1; Hsp72
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227368 representing NM_010479
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGAACACGGCGATCGGCATCGACCTGGGCACCACCTACTCGTGCGTGGGCGTGTTCCAGCACG
  GCAAGGTGGAGATCATCGCCAACGACCAGGGCAACCGCACGACCCCCAGCTACGTGGCCTTCACCGACAC
  CGAGCGCCTCATCGGAGACGCCGCCAAGAACCAGGTGGCGCTGAACCCGCAGAACACCGTGTTCGACGCG
  AAGCGGCTGATCGGCCGCAAGTTCGGCGATGCGGTGGTGCAGTCCGACATGAAGCACTGGCCCTTCCAGG
  TGGTGAACGACGGCGACAAGCCCAAGGTGCAGGTGAACTACAAGGGCGAGAGCCGGTCGTTCTTCCCGGA
  GGAGATCTCGTCCATGGTGCTGACGAAGATGAAGGAGATCGCTGAGGCGTACCTGGGCCACCCGGTGACC
  AACGCGGTGATCACGGTGCCCGCCTACTTCAACGACTCTCAGCGGCAGGCCACCAAGGACGCGGGCGTGA
  TCGCCGGTCTAAACGTGCTGCGGATCATCAACGAGCCCACGGCGGCCGCCATCGCCTACGGGCTGGACCG
  GACCGGCAAGGGCGAGCGCAACGTGCTCATCTTCGACCTGGGGGGCGGCACGTTCGACGTGTCCATCCTG
  ACGATCGACGACGGCATCTTCGAGGTGAAGGCCACGGCGGGCGACACGCACCTGGGAGGGGAGGACTTCG
  ACAACCGGCTGGTGAGCCACTTCGTGGAGGAGTTCAAGAGGAAGCACAAGAAGGACATCAGCCAGAACAA
  GCGCGCGGTGCGGCGGCTGCGCACTGCGTGTGAGAGGGCCAAGAGGACGCTGTCGTCCAGCACCCAGGCC
  AGCCTGGAGATCGACTCTCTGTTCGAGGGCATCGACTTCTACACATCCATCACGCGGGCGCGGTTCGAAG
  AGCTGTGCTCAGACCTGTTCCGCGGCACGCTGGAGCCCGTGGAGAAGGCCCTGCGCGACGCCAAGATGGA
  CAAGGCGCAGATCCACGACCTGGTGCTGGTGGGCGGCTCGACGCGCATCCCCAAGGTGCAGAAGCTGCTG
  CAGGACTTCTTCAACGGGCGCGACCTGAACAAGAGCATCAACCCGGACGAGGCGGTGGCCTACGGGGCGG
  CGGTGCAGGCGGCCATCCTGATGGGGGACAAGTCGGAGAACGTGCAGGACCTGCTGCTGCTGGACGTGGC
  GCCGCTGTCGCTGGGCCTGGAGACTGCGGGCGGCGTGATGACGGCGCTCATCAAGCGCAACTCCACCATC
  CCCACCAAGCAGACGCAGACCTTCACCACCTACTCGGACAACCAGCCCGGGGTGCTGATCCAGGTGTACG
  AGGGCGAGAGGGCCATGACGCGCGACAACAACCTGCTGGGGCGCTTCGAACTGAGCGGCATCCCGCCGGC
  GCCCAGGGGCGTGCCACAGATCGAGGTGACCTTCGACATCGACGCCAACGGCATCCTGAACGTCACGGCC
  ACCGACAAGACCACCGGCAAGGCCAACAAGATCACCATCACCAACGACAAGGGCCGCCTGAGCAAGGAGG
  AGATCGAGCGCATGGTGCAGGAGGCCGAGCGCTACAAGGCCGAGGACGAGGTGCAGCGCGACAGGGTGGC
  CGCCAAGAACGCGCTCGAATCCTATGCCTTCAACATGAAGAGCGCCGTGGAGGACGAGGGTCTCAAGGGC
  AAGCTCAGCGAGGCTGACAAGAAGAAGGTGCTGGACAAGTGCCAGGAGGTCATCTCCTGGCTGGACTCCA
  ACACGCTGGCCGACAAGGAGGAGTTCGTGCACAAGCGGGAGGAGCTGGAGCGGGTGTGCAGCCCCATCAT
  CAGTGGGCTGTACCAGGGTGCGGGTGCTCCTGGGGCTGGGGGCTTCGGGGCCCAGGCGCCCAAGGGAGCC
  TCTGGCTCAGGACCCACCATCGAGGAGGTGGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227368 representing NM_010479
  Red=Cloning site Green=Tags(s)
  
  MAKNTAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDA
  KRLIGRKFGDAVVQSDMKHWPFQVVNDGDKPKVQVNYKGESRSFFPEEISSMVLTKMKEIAEAYLGHPVT
  NAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSIL
  TIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQA
  SLEIDSLFEGIDFYTSITRARFEELCSDLFRGTLEPVEKALRDAKMDKAQIHDLVLVGGSTRIPKVQKLL
  QDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTI
  PTKQTQTFTTYSDNQPGVLIQVYEGERAMTRDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTA
  TDKTTGKANKITITNDKGRLSKEEIERMVQEAERYKAEDEVQRDRVAAKNALESYAFNMKSAVEDEGLKG
  KLSEADKKKVLDKCQEVISWLDSNTLADKEEFVHKREELERVCSPIISGLYQGAGAPGAGGFGAQAPKGA
  SGSGPTIEEVD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010479
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010479.1
• RefSeq Size: 2798 bp
• RefSeq ORF: 1926 bp
• Locus ID: 193740
• UniProt ID: Q61696
• Cytogenetics: 17 18.51 cM
• Gene Summary: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. The co-chaperones are of three types: J-domain co-chaperones such as HSP40s (stimulate ATPase hydrolysis by HSP70), the nucleotide exchange factors (NEF) such as BAG1/2/3 (facilitate conversion of HSP70 from the ADP-bound to the ATP-bound state thereby promoting substrate release), and the TPR domain chaperones such as HOPX and STUB1. Maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Its acetylation/deacetylation state determines whether it functions in protein refolding or protein degradation by controlling the competitive binding of co-chaperones HOPX and STUB1. During the early stress response, the acetylated form binds to HOPX which assists in chaperone-mediated protein refolding, thereafter, it is deacetylated and binds to ubiquitin ligase STUB1 that promotes ubiquitin-mediated protein degradation. Regulates centrosome integrity during mitosis, and is required for the maintenance of a functional mitotic centrosome that supports the assembly of a bipolar mitotic spindle. Enhances STUB1-mediated SMAD3 ubiquitination and degradation and facilitates STUB1-mediated inhibition of TGF-beta signaling. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response.[UniProtKB/Swiss-Prot Function]