Cyld (NM_001128170) Mouse Tagged ORF Clone

CAT#: MG227175

Cyld (tGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld) transcript variant 3, (10ug)

Product Images

Circular map for MG227175

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyld
• Synonyms: 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227175 representing NM_001128170
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGATTTTTTATCTGC
  TTCTTCAAGAATGCAGTGTAACAGACAAACAAACTCAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGG
  ACAGTACATCCAAGACCGTTCTGTGGGGCATTCAAGAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATT
  GGATTAAAAATCTTGGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG
  AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACT
  CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAG
  AAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG
  GAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGTTTCACGGATGGGGTATATCAAGGGAAGCAGCTTTT
  CCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCATTGGACAAACTAGAACTTATAGAAGATGATGACAAT
  GGATTGGAAAGTGATTTTGCAGGCCCAGGAGATACAATGCAGGTTGAACCTCCCCCTTTGGAAATAAACT
  CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTAATATTCTGTGATGTTTTACCAGG
  AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGGTTT
  GATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGAAAGTACAATTCTCCTGCACATCAATGACATCATCC
  CAGCTTTATCAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGG
  TGACAAAGGTTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAAC
  AGATCTGAATTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAATCCAAGTCCAAAAATCCAT
  GGTACATTGATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACAGAGATGTCTTCGGACTTCGGACATTC
  ATCTCCTCCACCGCAGCCTCCTTCCATGAACTCCTTGTCTAGCGAGAACAGATTCCACTCCTTACCCTTC
  AGCCTGACAAAGATGCCCAATACTAATGGCAGCATGGCTCATAGTCCACTCTCTCTGTCAGTGCAGTCTG
  TGATGGGGGAGCTGAACAGCACACCTGTCCAGGAGAGTCCACCCTTGCCCATCTCTTCTGGGAATGCACA
  CGGGCTAGAGGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCCCGTTCTATGGGGTTATCCGTTGGATT
  GGCCAGCCACCAGGGCTCAGTGACGTGCTAGCTGGACTGGAACTGGAAGATGAATGCGCAGGCTGTACAG
  ATGGAACTTTCAGGGGCACGCGGTATTTCACGTGTGCCCTGAAGAAGGCACTGTTTGTGAAACTGAAGAG
  CTGCAGACCGGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCAATCAAATTGAAAGGTGTAACTCTTTA
  GCATTTGGGGGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCCACCTAAAATGGAAAAGGAAGGTTTAG
  AGATAATGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACTTTATT
  CTGCTTATTTGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTAGACCCAAAGAGAAGAATGATATAGAG
  TATTACAGTGAGACTCAGGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATATGTGT
  GTGCCACAAAGATTATGAAACTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGTTTTACCTCTGA
  AGAAAAAGATCCTGAAGAATTCTTAAATATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGTTAAAA
  ATAAGATCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGAGAAAG
  TTGGAGTACCCACAATTCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCAGAGGC
  ACCATCATGCTTGATTATCCAGATGCCTCGGTTTGGAAAAGACTTTAAACTATTTAAAAAAATTTTTCCT
  TCCCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGGACTTG
  CGATGTACGAGTGCAGGGAGTGCTATGACGATCCGGACATCTCAGCTGGGAAGATCAAGCAGTTCTGTAA
  GACCTGCAGCACTCAGGTTCACCTTCATCCCAGGAGGTTGAATCATTCTTATCATCCAGTATCACTTCCC
  AAAGACTTGCCTGACTGGGACTGGAGACACGGCTGCATCCCCTGTCAGAAGATGGAGTTATTTGCTGTTC
  TCTGCATAGAAACGAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGATGACTCTGCCTGGCTTTTCTT
  TGACAGCATGGCGGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACGCCCTGCCCAGAA
  GTGGGAGAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGATTCCAGAAGGATTCAAGGCTGTG
  CGCGCAGACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCCTGTACAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227175 representing NM_001128170
  Red=Cloning site Green=Tags(s)
  
  MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRVPSTKGKKNQI
  GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRLRLSKGLQVDVGSPVKVQLRSGEE
  KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN
  GLESDFAGPGDTMQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF
  DGVQLCSFASVESTILLHINDIIPALSDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRN
  RSELFYTLNGSSVDSQQSKSKNPWYIDEVAEDPAKSLTEMSSDFGHSSPPPQPPSMNSLSSENRFHSLPF
  SLTKMPNTNGSMAHSPLSLSVQSVMGELNSTPVQESPPLPISSGNAHGLEVGSLAEVKENPPFYGVIRWI
  GQPPGLSDVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSL
  AFGGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSALDTVLLRPKEKNDIE
  YYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHDILRVEPLLK
  IRSAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFP
  SLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCSTQVHLHPRRLNHSYHPVSLP
  KDLPDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPE
  VGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128170
• ORF Size: 2865 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128170.2
• RefSeq Size: 8022 bp
• RefSeq ORF: 2868 bp
• Locus ID: 74256
• UniProt ID: Q80TQ2
• Cytogenetics: 8 C3
• Gene Summary: This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]