Ap2a1 (NM_001077264) Mouse Tagged ORF Clone

CAT#: MG227123

Ap2a1 (tGFP-tagged) - Mouse adaptor protein complex AP-2 alpha 1 subunit (Ap2a1) transcript variant 2, (10ug)

Product Images

Circular map for MG227123

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ap2a1
• Synonyms: Adtaa
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227123 representing NM_001077264
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGCTGTATCCAAAGGCGATGGCATGCGTGGGCTCGCCGTGTTCATCTCCGACATCCGGAACTGCA
  AGAGCAAAGAGGCTGAGATCAAGAGGATCAACAAGGAACTGGCCAACATCCGTTCCAAGTTCAAAGGGGA
  CAAGGCCTTGGATGGCTACAGTAAAAAGAAGTATGTGTGTAAGCTGCTCTTCATATTCCTGCTTGGCCAT
  GACATTGACTTTGGACATATGGAGGCCGTGAACCTGCTAAGCTCTAACAAGTACACGGAGAAGCAGATAG
  GGTACCTGTTCATCTCAGTCCTGGTGAACTCGAACTCAGAACTGATCCGGCTCATCAACAACGCTATCAA
  GAATGACCTGGCCAGTCGCAACCCCACCTTCATGTGCCTGGCCTTGCACTGTATCGCTAACGTGGGCAGC
  CGTGAGATGGGCGAGGCTTTTGCTGCAGACATTCCCCGAATCCTGGTGGCTGGGGACAGCATGGACAGTG
  TGAAGCAGAGTGCCGCCCTATGCCTACTGCGACTCTACAAGGCCTCGCCCGACTTGGTGCCCATGGGCGA
  GTGGACGGCACGTGTAGTGCACTTGCTCAATGATCAGCACATGGGAGTGGTCACAGCTGCTGTCAGCCTC
  ATCACCTGTCTCTGCAAGAAGAATCCGGATGACTTCAAGACCTGTATCTCCCTGGCTGTGTCTCGCTTAA
  GCCGGATCGTCTCCTCAGCCTCCACTGACCTCCAGGACTACACTTACTACTTCGTTCCTGCACCCTGGCT
  CTCTGTGAAGCTACTGCGGCTGCTCCAGTGTTACCCACCACCAGAGGATGCAGCCGTGAAAGGGCGGTTA
  GTGGAGTGTCTGGAGACTGTGCTCAACAAGGCCCAGGAGCCTCCCAAGTCCAAGAAGGTGCAGCACTCCA
  ACGCCAAGAACGCTATCCTCTTTGAGACCATTAGCCTCATCATCCACTATGACAGTGAGCCCAACCTCCT
  GGTCCGCGCCTGCAACCAGCTGGGCCAGTTCCTGCAGCACCGGGAGACTAACCTGCGCTACCTGGCCCTG
  GAGAGCATGTGCACGCTGGCCAGCTCCGAGTTCTCCCACGAGGCCGTCAAGACCCACATTGATACAGTTA
  TTAATGCCCTCAAGACGGAGCGGGACGTCAGTGTGAGGCAGCGGGCGGCTGATCTCCTGTATGCCATGTG
  TGACCGGAGCAATGCCAAGCAGATTGTGTCAGAGATGCTGCGGTACCTGGAGACTGCTGACTATGCCATC
  CGAGAGGAGATCGTGCTGAAGGTGGCCATCCTGGCTGAGAAGTATGCAGTGGACTACAGCTGGTACGTGG
  ACACCATCCTCAACCTCATCCGCATCGCGGGCGACTATGTGAGCGAGGAGGTGTGGTACCGCGTGCTGCA
  GATCGTCACCAACCGTGATGACGTCCAGGGTTATGCTGCCAAGACAGTGTTTGAGGCCCTCCAGGCCCCA
  GCCTGTCATGAGAACATGGTGAAGGTCGGTGGCTACATCCTTGGGGAGTTTGGGAACTTGATTGCTGGGG
  ACCCACGCTCCAGCCCACCAGTGCAGTTCTCGCTGCTGCACTCCAAGTTCCACCTGTGCAGCGTGGCCAC
  CCGCGCTCTGTTGCTGTCCACCTACATCAAGTTCATCAACCTCTTCCCTGAGACCAAGGCCACCATCCAA
  GGGGTTCTGCGTGCCGGCTCCCAGCTGCGAAATGCCGACGTGGAGCTACAGCAGCGGGCCGTGGAGTACC
  TCACCCTCAGCTCCGTAGCCAGCACCGATGTTCTGGCTACGGTGCTAGAAGAAATGCCCCCATTTCCCGA
  GCGGGAGTCGTCCATCTTGGCCAAGCTGAAGCGCAAGAAGGGCCCTGGGGCAGCCAGTGCCCTAGATGAC
  AGCCGCAGGGACACCAGCAGCAATGACATCAATGGGGGTGTGGAGCCCACCCCCAGCACTGTGTCGACCC
  CCTCACCCTCCGCGGACCTCTTAGGGCTGCGGGCAGCCCCTCCCCCTGCTGCACCCCCGGCTCCCGTAGG
  CGGGAACCTCCTGGTGGATGTCTTCTCTGACGGCCCCACTGCACAGCCCAGCCTGGGGCCCACTCCTGAG
  GAGGCCTTCCTCAGCCCAGGTCCTGAGGACATAGGCCCTCCCATTCCAGAAGCAGATGAACTGTTGAATA
  AGTTCGTGTGTAAGAATAGTGGGGTCTTGTTTGAGAACCAGCTGCTGCAGATTGGAGTCAAGTCTGAGTT
  CCGGCAGAACCTGGGCCGCATGTATCTCTTCTATGGCAACAAGACTTCTGTGCAGTTCCAGAACTTCTTG
  CCCACCGTGGTCCATCCTGGGGACCTCCAGACTCAGCTGGCGGTGCAGACCAAGCGTGTGGCGGCACAAG
  TGGACGGTGGCGCACAGGTGCAGCAAGTACTCAACATTGAGTGTCTGCGAGACTTCCTGACGCCGCCACT
  GTTGTCGGTGCGCTTCCGGTACGGTGGCACCGCCCAGTCCCTCACTCTGAAGCTCCCAGTGACCATCAAC
  AAATTCTTCCAGCCCACAGAGATGGCGGCCCAAGACTTTTTCCAGCGCTGGAAGCAGCTGAGCCTCCCCC
  TGCAGGAGGCACAGAAAATCTTCAAAGCCAACCACCCGATGGATGCTGAAGTTACTAAGGCCAAGCTTCT
  GGGGTTTGGCTCTGCTCTTCTGGACAATGTGGATCCCAACCCTGAGAACTTTGTGGGTGCTGGAATCATC
  CAGACGAAGGCCCTGCAGGTGGGGTGTCTGCTTCGGCTGGAGCCCAATGCCCAGGCCCAAATGTACCGTC
  TAACCCTGCGCACCAGCAAAGAGCCTGTCTCCCGTCACTTGTGTGAGCTGCTGGCCCAGCAGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227123 representing NM_001077264
  Red=Cloning site Green=Tags(s)
  
  MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGH
  DIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGS
  REMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSL
  ITCLCKKNPDDFKTCISLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRL
  VECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLAL
  ESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI
  REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAP
  ACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQ
  GVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDD
  SRRDTSSNDINGGVEPTPSTVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPE
  EAFLSPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFL
  PTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTIN
  KFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGII
  QTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001077264
• ORF Size: 2865 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001077264.1, NP_001070732.1
• RefSeq Size: 3391 bp
• RefSeq ORF: 2868 bp
• Locus ID: 11771
• UniProt ID: P17426
• Cytogenetics: 7 B3
• Gene Summary: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif (By similarity).[UniProtKB/Swiss-Prot Function]