Gli1 (NM_010296) Mouse Tagged ORF Clone

CAT#: MG227023

Gli1 (tGFP-tagged) - Mouse GLI-Kruppel family member GLI1 (Gli1), (10ug)

Product Images

Circular map for MG227023

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gli1
• Synonyms: AV235269; Zfp-5; Zfp5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227023 representing NM_010296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCTTCTTTAGGATTCCCACCTCAGATGAGTCATCAAAAAGGAACTTCACCTCCCTATGGAGTCC
  AGCCCTGTGTACCACATGACTCTACTCGGGGTTCAATGATGCTTCACCCCCAGTCCCGGGGACCACGTGC
  AACCTGCCAGCTGAAGTCAGAGCTGGATATGATGGTTGGCAAGTGCCCGGAGGACCCTTTGGAAGGGGAC
  ATGTCTAGCCCCAACTCCACAGGCACACAGGATCACCTGTTGGGGATGCTGGATGGGCGGGAGGACCTGG
  AGAGAGAGGAGAAGCCTGAGCCTGAGTCTGTGTATGAGACAGACTGCCGCTGGGATGGTTGCAGCCAGGA
  GTTCGATTCCCAGGAGCAGCTGGTGCACCACATCAACAGTGAGCATATCCACGGGGAGCGGAAGGAATTC
  GTGTGCCATTGGGGAGGTTGCTCCAGGGAGCTGAGGCCCTTCAAGGCCCAATACATGCTGGTGGTGCACA
  TGCGCAGACACACGGGCGAGAAGCCACACAAGTGCACGTTTGAAGGCTGTCGGAAGTCCTATTCACGCCT
  TGAAAACCTCAAGACGCACCTTCGGTCGCACACGGGTGAGAAGCCTTACATGTGTGAGCAAGAAGGTTGC
  AGCAAGGCCTTTAGCAATGCCAGTGACCGCGCCAAGCACCAGAATCGGACCCACTCCAATGAGAAGCCAT
  ACGTGTGCAAGCTCCCCGGCTGCACCAAGCGCTACACAGATCCCAGCTCGCTCCGCAAACACGTGAAGAC
  AGTGCATGGTCCGGATGCCCACGTGACCAAGCGGCATCGAGGGGATGGCCCCTTGCCACGGGCTCAGCCC
  CTCTCCACAGTGGAGCCCAAGCGGGAAAGGGAAGGAGGATCCGGCAGGGAAGAGAGCAGACTGACTGTGC
  CCGAGAGTGCCATGCCGCAGCAGAGCCCCGGAGCGCAGTCCTCTTGCAGCAGCGACCACTCCCCAGCAGG
  CAGTGCGGCCAACACGGACAGCGGCGTGGAGATGGCCGGCAACGCCGGGGGCAGCACTGAGGACTTGTCC
  AGCTTGGATGAAGGACCTTGTGTCTCGGCCACCGGACTCTCCACGCTTCGCCGCCTGGAGAACCTTAGGC
  TGGATCAGCTGCATCAGCTCCGGCCCATAGGGTCTCGGGGTCTCAAACTGCCCAGCTTAACCCACGCTGG
  CGCACCTGTGTCTCGCCGTCTGGGCCCCCCAGTCTCCCTGGACCGCCGCAGCAGCAGCTCCAGCAGCATG
  AGCTCTGCTTACACAGTCAGCCGCAGGTCCTCCCTGGCATCCCCTTTCCCGCCGGGAACCCCACCAGAGA
  ATGGGGCATCGTCACTACCTGGCCTCACACCTGCTCAGCACTACATGCTCCGTGCCAGATATGCTTCAGC
  CAGGGGGAGTGGCACCCCGCCCACTGCAGCTCACAGCCTGGATCGGATGGGAGGTCTTTCTGTTCCTCCT
  TGGAGAAGCCGAACCGAGTACCCGGGATACAACCCAAATGCAGGGGTCACTCGGAGGGCCAGTGACCCAG
  CCCGGGCTGCTGACCACCCAGCTCCAGCCAGAGTCCAGCGGTTCAAGAGCCTGGGATGTGTCCACACGCC
  CCCTAGTGTGGCAACGGGACGGAACTTCGATCCCCACCACCCTACCTCTGTCTATTCGCCACAGCCCCCC
  AGCATCACCGAAAATGTTGCCATGGATACTAGGGGGCTACAGGAGGAGCCAGAGGTTGGAACTTCTGTGA
  TGGGCAATGGTCTGAACCCATACATGGATTTTTCCTCCACTGATACTCTGGGATATGGGGGACCCGAGGG
  GACGGCAGCTGAGCCTTATGAAGCTAGGGGTCCAGGTTCCCTGCCTCTTGGGCCTGGTCCACCAACCAAC
  TATGGCCCTGGCCACTGTGCCCAGCAGGTCTCCTATCCTGATCCCACCCCAGAAAACTGGGGTGAGTTCC
  CTTCTCATGCTGGGGTGTACCCTAGCAATAAGGCTCCGGGTGCTGCCTATAGCCAGTGTCCTCGACTTGA
  GCATTATGGACAAGTGCAGGTAAAACCAGAACAAGGGTGCCCAGTGGGGTCTGACTCCACCGGATTGGCA
  CCCTGCCTCAATGCCCACCCCAGTGAAGGGTCCCCAGGCCCGCAGCCTCTGTTTTCACATCATCCCCAGC
  TCCCTCAGCCCCAGTATCCCCAGTCGGGTCCCTATCCTCAGCCTCCCCATGGTTATCTCTCAACAGAACC
  CAGGCTTGGCCTCAATTTCAACCCCTCCTCCTCTCATTCCACAGGACAGCTCAAAGCTCAGCTGGTGTGT
  AATTACGTTCAGTCGCAGCAGGAATTGTTGTGGGAGGGAAGAAACCGGGGAGGGCTCCCCAACCAGGAAC
  TCCCATACCAGAGCCCCAAGTTTCTGGGGGGTTCCCAAGTTAGTCAGAGCCCTGCCAAGACCCCAGCAGC
  AGCGGCGGCAGCATATGGATCTGGCTTTGCACCTGCTTCGGCCAATCACAAATCAGGCTCCTATCCTGCC
  CCTTCACCCTGCCATGAAACTTTCACCGTGGGAGTAAACAGGCCTTCCCACAGGCCAGCAGCACCACCCC
  GACTTCTGCCCCCGCTGTCCCCTTGCTATGGGCCCCTCAAGGTGGGGGATACCAACCCCAGCTGTGGCCA
  TCCTGAGGTGGGCAGGTTAGGAGCAGGCCCTGCCTTGTACCCTCCTCCTGAAGGGCAGGTGTGTAACGCT
  CTGGACTCTCTTGACCTGGACAACACTCAGCTGGACTTTGTGGCTATCCTAGATGAGGCCCAGGGCCTGA
  GCCCTCCTCTTTCCCATGAGCAAGGGGACAGCTCTAAAAACACCCCATCTCCCTCTGGGCCCCCCAACAT
  GGCAGTGGGTAACATGAGTGTCTTGCTGGGGTCTCTGCCTGGAGAGACACAATTCCTCAACTCTAGTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227023 representing NM_010296
  Red=Cloning site Green=Tags(s)
  
  MSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTRGSMMLHPQSRGPRATCQLKSELDMMVGKCPEDPLEGD
  MSSPNSTGTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEF
  VCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGC
  SKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQP
  LSTVEPKREREGGSGREESRLTVPESAMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLS
  SLDEGPCVSATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSM
  SSAYTVSRRSSLASPFPPGTPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRMGGLSVPP
  WRSRTEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHHPTSVYSPQPP
  SITENVAMDTRGLQEEPEVGTSVMGNGLNPYMDFSSTDTLGYGGPEGTAAEPYEARGPGSLPLGPGPPTN
  YGPGHCAQQVSYPDPTPENWGEFPSHAGVYPSNKAPGAAYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLA
  PCLNAHPSEGSPGPQPLFSHHPQLPQPQYPQSGPYPQPPHGYLSTEPRLGLNFNPSSSHSTGQLKAQLVC
  NYVQSQQELLWEGRNRGGLPNQELPYQSPKFLGGSQVSQSPAKTPAAAAAAYGSGFAPASANHKSGSYPA
  PSPCHETFTVGVNRPSHRPAAPPRLLPPLSPCYGPLKVGDTNPSCGHPEVGRLGAGPALYPPPEGQVCNA
  LDSLDLDNTQLDFVAILDEAQGLSPPLSHEQGDSSKNTPSPSGPPNMAVGNMSVLLGSLPGETQFLNSSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010296
• ORF Size: 3333 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010296.2, NP_034426.2
• RefSeq Size: 3662 bp
• RefSeq ORF: 3336 bp
• Locus ID: 14632
• UniProt ID: P47806
• Cytogenetics: 10 74.5 cM
• Gene Summary: Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.[UniProtKB/Swiss-Prot Function]