Ptch1 (NM_008957) Mouse Tagged ORF Clone

CAT#: MG227010

Ptch1 (tGFP-tagged) - Mouse patched homolog 1 (Ptch1), (10ug)

Product Images

Circular map for MG227010

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptch1
• Synonyms: A230106A15Rik; mes; Ptc; Ptc1; Ptch; wig
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG227010 representing NM_008957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGGCTGGTAACGCCGCCGGGGCCCTGGGCAGGCAGGCCGGCGGCGGGAGGCGCAGACGGACCG
  GGGGACCGCACCGCGCCGCGCCGGACCGGGACTATCTGCACCGGCCCAGCTACTGCGACGCCGCCTTCGC
  TCTGGAGCAGATTTCCAAGGGGAAGGCTACTGGCCGGAAAGCGCCGCTGTGGCTGAGAGCGAAGTTTCAG
  AGACTCTTATTTAAACTGGGTTGTTACATTCAAAAGAACTGCGGCAAGTTTTTGGTTGTGGGTCTCCTCA
  TATTTGGGGCCTTCGCTGTGGGATTAAAGGCAGCTAATCTCGAGACCAACGTGGAGGAGCTGTGGGTGGA
  AGTTGGTGGACGAGTGAGTCGAGAATTAAATTATACCCGTCAGAAGATAGGAGAAGAGGCTATGTTTAAT
  CCTCAACTCATGATACAGACTCCAAAAGAAGAAGGCGCTAATGTTCTGACCACAGAGGCTCTCCTGCAAC
  ACCTGGACTCAGCACTCCAGGCCAGTCGTGTGCACGTCTACATGTATAACAGGCAATGGAAGTTGGAACA
  TTTGTGCTACAAATCAGGGGAACTTATCACGGAGACAGGTTACATGGATCAGATAATAGAATACCTTTAC
  CCTTGCTTAATCATTACACCTTTGGACTGCTTCTGGGAAGGGGCAAAGCTACAGTCCGGGACAGCATACC
  TCCTAGGTAAGCCTCCTTTACGGTGGACAAACTTTGACCCCTTGGAATTCCTAGAAGAGTTAAAGAAAAT
  AAACTACCAAGTGGACAGCTGGGAGGAAATGCTGAATAAAGCCGAAGTTGGCCATGGGTACATGGACCGG
  CCTTGCCTCAACCCAGCCGACCCAGATTGCCCTGCCACAGCCCCTAACAAAAATTCAACCAAACCTCTTG
  ATGTGGCCCTTGTTTTGAATGGTGGATGTCAAGGTTTATCCAGGAAGTATATGCATTGGCAGGAGGAGTT
  GATTGTGGGTGGTACCGTCAAGAATGCCACTGGAAAACTTGTCAGCGCTCACGCCCTGCAAACCATGTTC
  CAGTTAATGACTCCCAAGCAAATGTATGAACACTTCAGGGGCTACGACTATGTCTCTCACATCAACTGGA
  ATGAAGACAGGGCAGCCGCCATCCTGGAGGCCTGGCAGAGGACTTACGTGGAGGTGGTTCATCAAAGTGT
  CGCCCCAAACTCCACTCAAAAGGTGCTTCCCTTCACAACCACGACCCTGGACGACATCCTAAAATCCTTC
  TCTGATGTCAGTGTCATCCGAGTGGCCAGCGGCTACCTACTGATGCTTGCCTATGCCTGTTTAACCATGC
  TGCGCTGGGACTGCTCCAAGTCCCAGGGTGCCGTGGGGCTGGCTGGCGTCCTGTTGGTTGCGCTGTCAGT
  GGCTGCAGGATTGGGCCTCTGCTCCTTGATTGGCATTTCTTTTAATGCTGCGACAACTCAGGTTTTGCCG
  TTTCTTGCTCTTGGTGTTGGTGTGGATGATGTCTTCCTCCTGGCCCATGCATTCAGTGAAACAGGACAGA
  ATAAGAGGATTCCATTTGAGGACAGGACTGGGGAGTGCCTCAAGCGCACCGGAGCCAGCGTGGCCCTCAC
  CTCCATCAGCAATGTCACCGCCTTCTTCATGGCCGCATTGATCCCTATCCCTGCCCTGCGAGCGTTCTCC
  CTCCAGGCTGCTGTGGTGGTGGTATTCAATTTTGCTATGGTTCTGCTCATTTTTCCTGCAATTCTCAGCA
  TGGATTTATACAGACGTGAGGACAGAAGATTGGATATTTTCTGCTGTTTCACAAGCCCCTGTGTCAGCAG
  GGTGATTCAAGTTGAGCCACAGGCCTACACAGAGCCTCACAGTAACACCCGGTACAGCCCCCCACCCCCA
  TACACCAGCCACAGCTTCGCCCACGAAACCCATATCACTATGCAGTCCACCGTTCAGCTCCGCACAGAGT
  ATGACCCTCACACGCACGTGTACTACACCACCGCCGAGCCACGCTCTGAGATCTCTGTACAGCCTGTTAC
  CGTCACCCAGGACAACCTCAGCTGTCAGAGTCCCGAGAGCACCAGCTCTACCAGGGACCTGCTCTCCCAG
  TTCTCAGACTCCAGCCTCCACTGCCTCGAGCCCCCCTGCACCAAGTGGACACTCTCTTCGTTTGCAGAGA
  AGCACTATGCTCCTTTCCTCCTGAAACCCAAAGCCAAGGTTGTGGTAATCCTTCTTTTCCTGGGCTTGCT
  GGGGGTCAGCCTTTATGGGACCACCCGAGTGAGAGACGGGCTGGACCTCACGGACATTGTTCCCCGGGAA
  ACCAGAGAATATGACTTCATAGCTGCCCAGTTCAAGTACTTCTCTTTCTACAACATGTATATAGTCACCC
  AGAAAGCAGACTACCCGAATATCCAGCACCTACTTTACGACCTTCATAAGAGTTTCAGCAATGTGAAGTA
  TGTCATGCTGGAGGAGAACAAGCAACTTCCCCAAATGTGGCTGCACTACTTTAGAGACTGGCTTCAAGGA
  CTTCAGGATGCATTTGACAGTGACTGGGAAACTGGGAGGATCATGCCAAACAATTATAAAAATGGATCAG
  ATGACGGGGTCCTCGCTTACAAACTCCTGGTGCAGACTGGCAGCCGAGACAAGCCCATCGACATTAGTCA
  GTTGACTAAACAGCGTCTGGTAGACGCAGATGGCATCATTAATCCGAGCGCTTTCTACATCTACCTGACC
  GCTTGGGTCAGCAACGACCCTGTAGCTTACGCTGCCTCCCAGGCCAACATCCGGCCTCACCGGCCGGAGT
  GGGTCCATGACAAAGCCGACTACATGCCAGAGACCAGGCTGAGAATCCCAGCAGCAGAGCCCATCGAGTA
  CGCTCAGTTCCCTTTCTACCTCAACGGCCTACGAGACACCTCAGACTTTGTGGAAGCCATAGAAAAAGTG
  AGAGTCATCTGTAACAACTATACGAGCCTGGGACTGTCCAGCTACCCCAATGGCTACCCCTTCCTGTTCT
  GGGAGCAATACATCAGCCTGCGCCACTGGCTGCTGCTATCCATCAGCGTGGTGCTGGCCTGCACGTTTCT
  AGTGTGCGCAGTCTTCCTCCTGAACCCCTGGACGGCCGGGATCATTGTCATGGTCCTGGCTCTGATGACC
  GTTGAGCTCTTTGGCATGATGGGCCTCATTGGGATCAAGCTGAGTGCTGTGCCTGTGGTCATCCTGATTG
  CATCTGTTGGCATCGGAGTGGAGTTCACCGTCCACGTGGCTTTGGCCTTTCTGACAGCCATTGGGGACAA
  GAACCACAGGGCTATGCTCGCTCTGGAGCACATGTTTGCTCCCGTTCTGGACGGTGCTGTGTCCACTCTG
  CTGGGTGTACTGATGCTTGCAGGGTCCGAATTTGATTTCATTGTCAGATACTTCTTTGCCGTCCTGGCCA
  TTCTCACCGTCTTGGGGGTTCTCAATGGACTGGTTCTGCTGCCTGTCCTCTTATCCTTCTTTGGACCGTG
  TCCTGAGGTGTCTCCAGCCAATGGCCTAAACCGACTGCCCACTCCTTCGCCTGAGCCGCCTCCAAGTGTC
  GTCCGGTTTGCCGTGCCTCCTGGTCACACGAACAATGGGTCTGATTCCTCCGACTCGGAGTACAGCTCTC
  AGACCACGGTGTCTGGCATCAGTGAGGAGCTCAGGCAATACGAAGCACAGCAGGGTGCCGGAGGCCCTGC
  CCACCAAGTGATTGTGGAAGCCACAGAAAACCCTGTCTTTGCCCGGTCCACTGTGGTCCATCCGGACTCC
  AGACATCAGCCTCCCTTGACCCCTCGGCAACAGCCCCACCTGGACTCTGGCTCCTTGTCCCCTGGACGGC
  AAGGCCAGCAGCCTCGAAGGGATCCCCCTAGAGAAGGCTTGCGGCCACCCCCCTACAGACCGCGCAGAGA
  CGCTTTTGAAATTTCTACTGAAGGGCATTCTGGCCCTAGCAATAGGGACCGCTCAGGGCCCCGTGGGGCC
  CGTTCTCACAACCCTCGGAACCCAACGTCCACCGCCATGGGCAGCTCTGTGCCCAGCTACTGCCAGCCCA
  TCACCACTGTGACGGCTTCTGCTTCGGTGACTGTTGCTGTGCATCCCCCGCCTGGACCTGGGCGCAACCC
  CCGAGGGGGGCCCTGTCCAGGCTATGAGAGCTACCCTGAGACTGATCACGGGGTATTTGAGGATCCTCAT
  GTGCCTTTTCATGTCAGGTGTGAGAGGAGGGACTCAAAGGTGGAGGTCATAGAGCTACAGGACGTGGAAT
  GTGAGGAGAGGCCGTGGGGGAGCAGCTCCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG227010 representing NM_008957
  Red=Cloning site Green=Tags(s)
  
  MASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQ
  RLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFN
  PQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLY
  PCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDR
  PCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSRKYMHWQEELIVGGTVKNATGKLVSAHALQTMF
  QLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVAPNSTQKVLPFTTTTLDDILKSF
  SDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLP
  FLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFS
  LQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTEPHSNTRYSPPPP
  YTSHSFAHETHITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDNLSCQSPESTSSTRDLLSQ
  FSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRE
  TREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFSNVKYVMLEENKQLPQMWLHYFRDWLQG
  LQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLT
  AWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKV
  RVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMT
  VELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTL
  LGVLMLAGSEFDFIVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGLNRLPTPSPEPPPSV
  VRFAVPPGHTNNGSDSSDSEYSSQTTVSGISEELRQYEAQQGAGGPAHQVIVEATENPVFARSTVVHPDS
  RHQPPLTPRQQPHLDSGSLSPGRQGQQPRRDPPREGLRPPPYRPRRDAFEISTEGHSGPSNRDRSGPRGA
  RSHNPRNPTSTAMGSSVPSYCQPITTVTASASVTVAVHPPPGPGRNPRGGPCPGYESYPETDHGVFEDPH
  VPFHVRCERRDSKVEVIELQDVECEERPWGSSSN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008957
• ORF Size: 4302 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008957.3
• RefSeq Size: 4305 bp
• RefSeq ORF: 4305 bp
• Locus ID: 19206
• UniProt ID: Q61115
• Cytogenetics: 13 32.8 cM
• Gene Summary: Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.[UniProtKB/Swiss-Prot Function]