Csf1 (NM_007778) Mouse Tagged ORF Clone

CAT#: MG226827

  • TrueORF®

Csf1 (tGFP-tagged) - Mouse colony stimulating factor 1 (macrophage) (Csf1) transcript variant 1, (10ug)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_007778" in other vectors (5)

Reconstitution Protocol

USD 714.00

3 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (4)
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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Csf1
Synonyms C87615; Csfm; MCSF; op
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG226827 representing NM_007778
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGCGCGGGGCGCCGCGGGGCGCTGCCCTTCTTCGACATGGCTGGGCTCCCGGCTGCTGCTGGTCT
GTCTCCTCATGAGCAGGAGTATTGCCAAGGAGGTGTCAGAACACTGTAGCCACATGATTGGGAATGGACA
CCTGAAGGTCCTGCAGCAGTTGATCGACAGTCAAATGGAGACTTCATGCCAGATTGCCTTTGAATTTGTA
GACCAGGAACAGCTGGATGATCCTGTTTGCTACCTAAAGAAGGCCTTTTTTCTGGTACAAGACATAATAG
ATGAGACCATGCGCTTTAAAGACAACACCCCCAATGCTAACGCCACCGAGAGGCTCCAGGAACTCTCCAA
TAACCTGAACAGCTGCTTCACCAAGGACTATGAGGAGCAGAACAAGGCCTGTGTCCGAACTTTCCATGAG
ACTCCTCTCCAGCTGCTGGAGAAGATCAAGAACTTCTTTAATGAAACAAAGAATCTCCTTGAAAAGGACT
GGAACATTTTTACCAAGAACTGCAACAACAGCTTTGCTAAGTGCTCTAGCCGAGATGTGGTGACCAAGCC
TGATTGCAACTGCCTGTACCCTAAAGCCACCCCTAGCAGTGACCCGGCCTCTGCCTCCCCTCACCAGCCC
CCCGCCCCCTCCATGGCCCCTCTGGCTGGCTTGGCTTGGGATGATTCTCAGAGGACAGAGGGCAGCTCCC
TCTTGCCCAGTGAGCTTCCCCTTCGCATAGAGGACCCAGGCAGTGCCAAGCAGCGACCACCCAGGAGTAC
CTGCCAGACCCTCGAGTCAACAGAGCAACCAAACCATGGGGACAGACTCACTGAGGACTCACAACCTCAT
CCTTCTGCGGGGGGGCCCGTCCCTGGGGTGGAAGACATTCTTGAATCTTCACTGGGCACTAACTGGGTCC
TAGAAGAAGCTTCTGGAGAGGCTAGTGAGGGATTTTTGACCCAGGAAGCAAAGTTTTCCCCCTCCACGCC
TGTAGGGGGCAGCATCCAGGCAGAGACTGACAGACCCAGGGCCCTCTCAGCATCTCCATTCCCTAAATCA
ACAGAGGACCAAAAGCCAGTGGATATAACAGACAGGCCGTTGACAGAGGTGAACCCTATGAGACCCATTG
GCCAGACACAGAATAATACTCCTGAGAAGACTGATGGTACATCCACGCTGCGTGAAGACCACCAGGAGCC
AGGCTCTCCCCATATTGCGACACCGAATCCCCAACGAGTCAGCAACTCAGCCACCCCCGTTGCTCAGTTA
CTGCTTCCCAAAAGCCACTCTTGGGGCATTGTGCTGCCCCTTGGGGAGCTTGAGGGCAAGAGAAGTACCA
GGGATCGAAGGAGCCCCGCAGAGCTGGAAGGAGGATCAGCAAGTGAGGGGGCAGCCAGGCCTGTGGCCCG
TTTTAATTCCATTCCTTTGACTGACACAGGCCATGTGGAGCAGCATGAGGGATCCTCTGACCCCCAGATC
CCTGAGTCTGTCTTCCACCTGCTGGTGCCGGGCATCATCCTAGTCTTGCTGACTGTTGGGGGCCTCCTGT
TCTACAAGTGGAAGTGGAGGAGCCATCGAGACCCTCAGACATTGGATTCTTCTGTGGGGCGACCAGAGGA
CAGCTCCCTGACCCAGGATGAGGACAGACAGGTGGAACTGCCAGTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG226827 representing NM_007778
Red=Cloning site Green=Tags(s)

MTARGAAGRCPSSTWLGSRLLLVCLLMSRSIAKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFV
DQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHE
TPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKPDCNCLYPKATPSSDPASASPHQP
PAPSMAPLAGLAWDDSQRTEGSSLLPSELPLRIEDPGSAKQRPPRSTCQTLESTEQPNHGDRLTEDSQPH
PSAGGPVPGVEDILESSLGTNWVLEEASGEASEGFLTQEAKFSPSTPVGGSIQAETDRPRALSASPFPKS
TEDQKPVDITDRPLTEVNPMRPIGQTQNNTPEKTDGTSTLREDHQEPGSPHIATPNPQRVSNSATPVAQL
LLPKSHSWGIVLPLGELEGKRSTRDRRSPAELEGGSASEGAARPVARFNSIPLTDTGHVEQHEGSSDPQI
PESVFHLLVPGIILVLLTVGGLLFYKWKWRSHRDPQTLDSSVGRPEDSSLTQDEDRQVELPV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_007778
ORF Size 1656 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_007778.4, NP_031804.3
RefSeq Size 4192 bp
RefSeq ORF 1659 bp
Locus ID 12977
UniProt ID P07141
Cytogenetics 3 46.83 cM
Gene Summary Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.[UniProtKB/Swiss-Prot Function]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.