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Chd8 (NM_201637) Mouse Tagged ORF Clone
CAT#: MG226422
- TrueORF®
Chd8 (tGFP-tagged) - Mouse chromodomain helicase DNA binding protein 8 (Chd8), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_201637" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Chd8 |
| Synonyms | 5830451P18Rik; AU015341; Chd-8; Du; Duplin; HELSNF1; mKIAA1564 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226422 representing NM_201637, codon optimized.
Due to the complexity of NM_201637, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGACCCCATCATGGATCTTTTTGATGACCCAAACTTATTTGGCCTGGACTCTCTGACTGATGACA GCTTTAATCAGGTCACTCAAGACCCCATTGAAGAAGCACTTGGACTGCCAAGCTCTCTGGACTCCTTGGA TCAGATGAACCAAGATGGTGGAGGTGGTGATGTGGGGAATTCATCAGCAAGTGACCTGGTTCCTCCACCA GAGGAAACAGCTTCCACTGAACTTCCCAAAGAATCCACAGCTCCAGCTCCAGAATCCTTAACCTTGCATG ATTATACCACTCAGCCCACCAGCCAGGAGCAGCCAGCCCAGCCTGTCTTGCAGACATCAACACCAACAGC AGGACTCTTGCAGGTCTCCAAGAGCCAGGAGATCTTGAGCCAAGGGAATCCTTTCATGGGTGTCTCTGCC ACAGGTGTCTCCCCCAGTAATACTGGAGGACAACCATCTCAGTCAGCTCCTAAGATTGTTATTCTTAAAG CTCCACCAAACTCCTCCGTCACAGGCACCCATGTGGCACAAATTCAGGCCCAAGGTATCACCAGTACAGC TCAGCCCCTAGTGGCTGGGACAGCCAACGGTGGAAAAGTCACTTTTACCAAAGTGCTGACTGGTACACCC CTTCGACCAGGTGTATCCATTGTCTCTGGTAATACAGTGTTGGCCACGAAGGTCCCTGGGAACCAGGCTG CTGTTCAGCGCATTGTCCAGCCAAGCCGACCAGTAAAACAGCTGGTCCTCCAGCCAGTAAAGGGTTCAGC TCCTGCTGGGAATCCTGGGGCTGCCGGGCCCCCACTGAAGCCTGCAGTTACACTGACGTCTACACCTACC CAGGGTGAATCGAAACGCATCACTTTGGTCCTTCAACAGCCACAGTCCGGAGGTCCCCAAGGACATCGGC ATGTTGTGTTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAGCTAGCAGCCTTGACTCAAGC CAAGAATGCCCAGGGGCAGCCTGCCAAAGTAGTAACTATTCAGCTGCAGGTGCAGCAGCCACAGCAGAAA ATCCAGATTGTACCTCAGCCTCCATCATCACAACCACAGCCACAGCCGCAGCCGCCACCCTCAGCCCAGC CGTTGACCCTGTCCTCTGTGCAGCAGGCTCAGATAATGGGGCCAGGCCAAAACCCAGGGCAGAGACTTTC TGTACCGCTCAAGATGGTGCTGCAGCCACAGGCTGGCTCTTCTCAGGGAGCCTCTTCTGGACTCTCTGTG GTTAAAGTTCTAAGTGCCAGTGAAGTGGCAGCCCTGTCATCCCCAGCAAGCTGTGCCCCTCATACTGCAG GGAAGACTGGGATGGAGGAGAACCGCAGGTTGGAGCACCAGAAAAAGCAAGAGAAAGCAAATCGGATTGT GGCAGAGGCCATTGCTAGGGCCCGGGCCAGGGGCGAGCAAAACATACCTCGAGTCCTGAATGAGGATGAG CTGCCTAGTGTCCGGCCAGAGGAGGAGGGTGAAAAGAAACGCAGGAAGAAGAGCAGTGGGGAAAGGCTGA AGGAGGAGAAGCCAAAGAAGAGCAAGACTGCTGCTGCCTCCAAAACGAAGGGCAAGAGTAAGCTAAACAC CATCACTCCTGTAGTGGGCAAGAAGAGAAAGCGTAACACATCGTCTGACAACTCTGACGTGGAAGTTATG CCTGCACAATCGCCCCGGGAGGACGAAGAGAGCAGCATCCAGAAGAGACGTTCAAACCGCCAAGTTAAGC GAAAAAAATATACAGAGGACCTGGATATAAAGATAACAGACGATGAAGAGGAGGAGGAGGTCGATGTAAC TGGTCCAATAAAACCCGAGCCCATCCTCCCAGAACCAGTGCAAGAGCCTGATGGCGAGACTCTTCCTTCC ATGCAGTTCTTTGTGGAGAATCCTAGTGAAGAAGATGCAGCTATTGTGGACAAAGTGCTTTCCATGCGAG TTGTGAAGAAAGAGCTTCCTTCTGGACAATACACTGAGGCTGAAGAATTCTTCGTCAAGTACAAGAACTA CTCTTATCTGCACTGTGAGTGGGCAACGATCTCCCAGCTGGAGAAGGATAAGAGGATCCACCAGAAACTA AAACGGTTCAAAACAAAAATGGCTCAGATGAGACACTTCTTTCATGAAGATGAAGAGCCTTTCAATCCAG ACTACGTAGAGGTGGATAGGATACTGGACGAGTCTCACAGTGTTGACAAGGATAATGGTGAGCCTGTAAT TTACTACCTGGTAAAATGGTGCTCTCTGCCCTATGAGGACAGTACGTGGGAGCTAAAAGAGGATGTTGAT GAGGGCAAGATTCGGGAATTTAAACGGATCCAGTCAAGGCACCCAGAACTGAGAAGGGTGAATCGTCCAC AGGCAAATGCCTGGAAGAAATTGGAGTTATCACATGAATATAAAAACAGAAACCAATTACGGGAATATCA GTTAGAAGGGGTCAACTGGCTCCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAAATG GGATTGGGCAAAACTATTCAGTCAATCGCCTTCTTGCAGGAGGTATATAATGTAGGCATCCATGGTCCCT TTTTGGTCATTGCTCCATTGTCCACCATTACTAACTGGGAGCGTGAATTCAATACATGGACAGAGATGAA CACTATTGTGTACCATGGCAGCCTGGCCAGCCGGCAGATGATCCAGCAGTATGAAATGTACTGTAAAGAC TCACGGGGTCGCCTCATCCCTGGTGCATATAAGTTTGATGCCTTGATCACCACTTTTGAGATGATTTTGT CAGACTGTCCTGAGCTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATAGGCTGAAGAA CCGTAATTGCAAGCTACTTGATAGTCTCAAGCACATGGACCTGGAGCATAAAGTGTTACTCACAGGGACA CCGTTACAAAATACCGTAGAGGAACTGTTCAGTCTACTGCATTTCTTGGAACCTTCTCAGTTCCCCTCAG AATCAGAATTCCTTAAGGATTTTGGGGATCTCAAGACCGAGGAGCAGGTTCAAAAGCTTCAGGCCATTCT AAAGCCAATGATGCTGAGGAGACTCAAAGAGGATGTTGAAAAAAATTTGGCTCCCAAACAGGAAACTATT ATTGAAGTAGAGCTAACCAACATCCAGAAGAAATACTACAGAGCTATTTTAGAGAAGAATTTCTCCTTCC TTTCCAAAGGGGCAGGTCATACCAATATGCCTAATCTACTGAACACAATGATGGAGCTACGAAAGTGCTG CAATCACCCGTACCTCATCAATGGTGCAGAAGAGAAAATCCTGATGGAATTTCGGGAAGCTTGCCATATT ATACCTCAAGATTTCCACCTGCAGGCTATGGTTCGGTCAGCTGGCAAGTTAGTTCTCATTGACAAGTTAC TTCCAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCCCAGATGGTACGCTGTCTAGACATTCT GGAGGATTATCTGATCCAGAGGAGGTACTTATATGAACGTATTGATGGGCGAGTTAGAGGCAACCTTCGA CAAGCTGCTATCGACCGCTTTAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTGCTATGTACCCGTGCTG GTGGACTTGGTATTAATCTTACAGCTGCTGATACCTGTATTATCTTTGATTCAGACTGGAATCCACAGAA TGACCTGCAGGCCCAAGCACGTTGTCATCGAATTGGACAGAGCAAAGCTGTGAAGGTGTACCGACTCATC ACTCGTAATTCCTATGAGAGAGAGATGTTTGATAAAGCTAGCCTCAAGTTGGGATTGGATAAAGCTGTGC TTCAGTCCATGAGTGGTCGGGATGGCAACATTACTGGAATTCAACAGTTCTCCAAGAAAGAGATTGAAGA TCTCTTAAGGAAAGGTGCCTATGCGGCCATAATGGAGGAAGACGATGAGGGTTCTAAGTTTTGTGAGGAG GACATAGATCAGATCTTACTGAGACGGACCACAACCATCACTATTGAGTCTGAAGGGAAAGGTTCTACTT TTGCCAAGGCAAGCTTTGTTGCTTCTGAAAATAGGACAGATATTTCCCTGGATGATCCAAACTTTTGGCA AAAATGGGCCAAAAAGGCAGACCTAGACATGGATCTGCTGAATAGTAAGAATAACTTAGTGATCGACACA CCTCGAGTTCGGAAACAGACTCGACACTTCAGCACTTTGAAAGATGATGACCTGGTTGAGTTTTCTGATT TGGAAAGTGAAGATGATGAACGGCCACGTTCCCGAAGACATGACCGGCATCACACCTATGGGCGCACTGA CTGCTTCCGGGTAGAAAAGCATCTCCTGGTATATGGTTGGGGACGTTGGAGAGATATTTTGTCTCACGGA CGATTTAAGCGGCGCATGACTGAAAGAGATGTTGAGACAATATGCCGTGCCATCCTCGTGTACTGTCTTC TACACTACCGTGGGGATGAAAACATCAAAAGCTTCATTTGGGACTTGATTAGCCCTGCTGAAAATGGCAA GACAAAAGAATTGCAGAATCATTCAGGTCTGTCTATCCCTGTGCCCCGTGGGCGTAAGGGGAAAAAAGTC AAGTCACAAAGTACTTTTGACATTCATAAGGCAGACTGGATCCGGAAATACAACCCTGATACTCTGTTCC AAGATGAGAGTTATAAGAAGCATTTGAAACATCAGTGTAACAAGGTACTGTTGAGAGTCCGGATGCTCTA CTACCTGAGACAGGAGGTTATTGGAGACCAGGCAGAGAAGGTGTTAGGAGGTGCCATAGCCAGTGAGATT GACATATGGTTCCCAGTGGTGGATCAGCTGGAGGTTCCTACAACTTGGTGGGATAGTGAGGCTGACAAAT CCCTGCTCATTGGCGTTTTTAAGCATGGATACGAAAAGTATAATACTATGCGGGCCGATCCGGCTTTGTG CTTCCTGGAGAAGGCAGGCCGGCCAGACGACAAAGCAATTGCTGCCGAACATAGAGTGCTCGATAATTTC TCTGACCTTGTCGAGGGCATTGATTTCGATAAGGACTGCGAGGACCCCGAATACAAGCCACTGCAGGGGC CCCCCAAGGACCCTGATGATGAGGGTGACCCCCTGATGATGATGGATGAGGAGATCTCTGTGATCGATGG CGAAGAAGCACAGGTAACACAGCAGCCGGGACACCTGTTTTGGCCTCCAGGAAGTGCCCTTACCGCTAGA CTGCGGCGGCTGGTGACTGCTTACCAAAGATCTTATAAGCGGGAGCAGATGAAAATGGAAGCTGCCGAGC GCGGGGATAGAAGACGGCGGAGGTGTGAAGCCGCCTTTAAGCTGAAAGAAATCGCTAGGCGGGAAAAGCA GCAGAGGTGGACACGCAGAGAGCAGACTGATTTCTATAGGGTCGTGAGCACCTTCGGAGTCGAGTACGAT CCTGACAACATGCAATTTCATTGGGACCGGTTTAGAACATTCGCCAGACTGGACAAGAAGACCGACGAGT CCTTGACTAAGTATTTCCACGGGTTTGTCGCAATGTGCAGACAGGTGTGCCGACTGCCCCCTGCCGCCGG CGATGAGCCCCCTGACCCCAACCTGTTTATTGAACCTATTACCGAAGAGAGGGCTAGCCGGACTTTGTAT CGGATTGAGCTGCTGAGGCGCCTGAGGGAACAGGTGCTGTGTCACCCCCTGCTGGAAGATCGCCTGGCAC TGTGTCAACCCCCGGGCCTCGAGCTGCCTAAGTGGTGGGAGCCAGTCCGCCATGATGGAGAATTGCTGAG AGGAGCAGCTCGACATGGCGTCTCCCAGACCGACTGCAATATCATGCAAGATCCCGACTTCAGCTTCCTT GCCGCGCGGATGAACTACATGCAGAACCATCAGGCTGGGGCTTCCGCAGCGAGCCTGTCACGATGCTCCA CCCCTCTGCTGCATCAGCAATGCACTAGTCGAACCGCAAGCCCTTCCCCACTTAGACCCGATGCCCCAGT TGAGAAATCCCCCGAGGAGTCTACCGTTCAGGTGCCCAATCTCGAATCCCTCACGTTGAAACTTGAGGAC GAGGTCGTCGCCAGAAGCCGACTTACTAGCCAGGACTACGAAGTAAGAGTGGGCAGCTCAGATACCGCTC CTCTGTCTCGATCCGTTCCTCCTGTGAAATTGGAAGATGAGGATGATTCAGACAGCGAGCTGGATCTGAG CAAGCTTAGTCCATCCAGTAGTAGCAGTAGTTCATCTTCCTCAAGTTCCAGCAGCACAGATGAGTCTGAG GACGAGAAGGAGGAGAAGCTGACCGCCGACAGGAGCCGACCCAAACTGTATGACGAAGAGAGTCTGCTCA GTCTGACCATGTCCCAGGACGGATTTCCCAATGAAGATGGCGAACAGATGACTCCAGAACTGCTGCTGCT CCAGGAGCGACAGCGGGCCAGCGAATGGCCCAAAGATAGGGTGCTTATCAATCGGATCGATCTTGTGTGT CAGGCCGTTCTTTCCGGGAAGTGGCCCTCTAATAGAAGATCTCAGGAAGTAACAGCTGGGGGCATTCTGG GCCCGGGAAACCATCTCCTGGACTCTCCATCCCTCACACCTGGTGAGGACGGTGATTCTCCTGTCCCAAC CCCACGCAGCGGTAGTGCCGCAAGTATGGCAGAGGAAGAGGCCAGCGCCGTAACTACGGCTGCAGCCCAG TTTACCAAGCTTCGACGCGGTATGGACGAAAAAGAATTCACAGTACAGATTAAAGACGAGGAGGGGCTTA AACTCACTTTTCAGAAGCACCGGCTGATGGCAAACGGCGTAATGGGTGATGGTCATCCCCTGTTCCATAA GAAAAAGGGTAATCGGAAGAAGCTGGTTGAGCTGGAAGTGGAGTGTATGGAGGAGCCGAACCACCTGGAT CTCGATCTGGAAACTAGGATTCCAGTAATCAATAAGGTAGATGGCACTCTGCTGGTTGGAGATGAAGCCC CACGCAGAGCCGAACTGGAAATGTGGCTGCAGGGACACCCTGAGTTCGCAGTCGATCCTAGATTCTTGGC TTACATGGAGGAAAGGCGGAAGCAGAAATGGCAGCGGTGCAAAAAAAATAATAAGGCCGAGCTGAACTGT CTGGGCATGGAGCCCGTCCAACCAGCAAATTCTAGAAACGGGAAGAAAGGGCATTATGCCGAGACGGCCT TCAATCGGGTCCTGCCTGGCCCCGTTGCACCGGAAAACTCCAAAAAAAGAGTGCGGCGGACTCGGCCAGA TTTGAGTAAGATGATGGCACTGATGCAGGGGGGCAGTACGGGCAGTCTCTCCTTGCACAATACTTTCCAG CATAGCTCAAGCAATCTGCAAAGCGTTTCATCACTGGGGCACTCCAGTACAACATCTGCTTCATTGCCAT TCATGCCATTCGTGATGGGAGCCGCAGCACCACCCCACGTAGATTCCTCCACCATGCTCCACCACCACCA CCATCACCCCCATCCGCATCACCATCATCATCATCACCCCGGCCTTCGGACTACCGGATACCCAAGCAGC CCAGCCACTACGACTAGCGGCACTGCTCTCAGGCTCCCTACCCTGCAACCTGAGGATGACGATGAGGAGG AAGACGAGGAGGACGATGACCTGTCCCAGGGATACGACTCCAGCGAACGGGACTTCAGCCTTATTGACGA TCCCATGATGCCTGCAAATAGCGATAGCTCCGAGGATGCAGATGAC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226422 representing NM_201637
Red=Cloning site Green=Tags(s) MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASDLVPPP EETASTELPKESTAPAPESLTLHDYTTQPTSQEQPAQPVLQTSTPTAGLLQVSKSQEILSQGNPFMGVSA TGVSPSNTGGQPSQSAPKIVILKAPPNSSVTGTHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTP LRPGVSIVSGNTVLATKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGAAGPPLKPAVTLTSTPT QGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQK IQIVPQPPSSQPQPQPQPPPSAQPLTLSSVQQAQIMGPGQNPGQRLSVPLKMVLQPQAGSSQGASSGLSV VKVLSASEVAALSSPASCAPHTAGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDE LPSVRPEEEGEKKRRKKSSGERLKEEKPKKSKTAAASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVM PAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPS MQFFVENPSEEDAAIVDKVLSMRVVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKL KRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSVDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVD EGKIREFKRIQSRHPELRRVNRPQANAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEM GLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKD SRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGT PLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETI IEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILMEFREACHI IPQDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLR QAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLI TRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEE DIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDT PRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHTYGRTDCFRVEKHLLVYGWGRWRDILSHG RFKRRMTERDVETICRAILVYCLLHYRGDENIKSFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKV KSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEI DIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNF SDLVEGIDFDKDCEDPEYKPLQGPPKDPDDEGDPLMMMDEEISVIDGEEAQVTQQPGHLFWPPGSALTAR LRRLVTAYQRSYKREQMKMEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYD PDNMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLY RIELLRRLREQVLCHPLLEDRLALCQPPGLELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFL AARMNYMQNHQAGASAASLSRCSTPLLHQQCTSRTASPSPLRPDAPVEKSPEESTVQVPNLESLTLKLED EVVARSRLTSQDYEVRVGSSDTAPLSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESE DEKEEKLTADRSRPKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVC QAVLSGKWPSNRRSQEVTAGGILGPGNHLLDSPSLTPGEDGDSPVPTPRSGSAASMAEEEASAVTTAAAQ FTKLRRGMDEKEFTVQIKDEEGLKLTFQKHRLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLD LDLETRIPVINKVDGTLLVGDEAPRRAELEMWLQGHPEFAVDPRFLAYMEERRKQKWQRCKKNNKAELNC LGMEPVQPANSRNGKKGHYAETAFNRVLPGPVAPENSKKRVRRTRPDLSKMMALMQGGSTGSLSLHNTFQ HSSSNLQSVSSLGHSSTTSASLPFMPFVMGAAAPPHVDSSTMLHHHHHHPHPHHHHHHHPGLRTTGYPSS PATTTSGTALRLPTLQPEDDDEEEDEEDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_201637 |
| ORF Size | 7746 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_201637.2, NP_963999.2 |
| RefSeq Size | 8190 bp |
| RefSeq ORF | 7749 bp |
| Locus ID | 67772 |
| UniProt ID | Q09XV5 |
| Cytogenetics | 14 C2 |
| Gene Summary | This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which is common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. In mammals, this gene has been shown to function in several processes including transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Knockout of this gene causes early embryonic lethality due to widespread apoptosis. Heterozygous loss of function mutations result in autism spectrum disorder-like behaviors that include increased anxiety, repetitive behavior, and altered social behavior. [provided by RefSeq, Dec 2016] |
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