Ptger4 (NM_001136079) Mouse Tagged ORF Clone

CAT#: MG226255

Ptger4 (tGFP-tagged) - Mouse prostaglandin E receptor 4 (subtype EP4) (Ptger4) transcript variant 1, (10ug)

Product Images

Circular map for MG226255

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ptger4
• Synonyms: EP4; Ptgerep4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226255 representing NM_001136079
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCCCCGGAGTCAACGCGTCCTTCTCCTCCACTCCGGAGAGGCTGAACAGCCCGGTGACCATTC
  CCGCAGTGATGTTCATCTTCGGGGTGGTGGGCAACCTGGTGGCCATCGTAGTATTGTGCAAGTCGCGCAA
  GGAGCAGAAAGAGACGACCTTTTACACTCTAGTATGTGGGCTGGCTGTCACTGACCTTCTGGGCACCTTG
  TTGGTAAGCCCGGTGACCATCGCCACATACATGAAGGGCCAGTGGCCCGGAGACCAGGCACTGTGTGACT
  ATAGCACCTTCATCCTACTTTTCTTCGGTCTGTCGGGTCTCAGCATCATCTGTGCCATGAGCATCGAGCG
  CTACCTGGCCATCAACCACGCCTACTTCTACAGCCACTACGTGGACAAGCGGCTGGCCGGCCTCACACTC
  TTCGCCATCTATGCATCTAACGTGCTGTTCTGCGCGCTGCCCAACATGGGCCTGGGCAGATCCGAGCGGC
  AGTACCCGGGCACCTGGTGCTTCATCGACTGGACCACCAACGTAACGGCCTACGCCGCCTTCTCTTACAT
  GTACGCCGGCTTCAGCTCCTTCCTCATCCTTGCCACCGTGCTCTGCAACGTGCTGGTGTGCGGCGCGCTG
  CTCCGCATGCACCGCCAGTTCATGCGCCGCACCTCGTTGGGCACGGAGCAGCACCATGCGGCTGCCGCCG
  CCGCGGTAGCTTCGGTGGCCTGTCGGGGCCACGCTGGGGCCTCCCCAGCCCTGCAGCGCCTCAGCGACTT
  TCGCCGCCGCAGGAGTTTCCGGCGCATCGCGGGTGCGGAGATCCAGATGGTCATCTTACTCATCGCCACC
  TCTCTGGTGGTGCTCATCTGCTCCATTCCGCTCGTGGTGCGAGTGTTCATTAACCAGTTATATCAGCCAA
  ACGTGGTGAAAGACATCAGCAGAAACCCAGATTTGCAGGCCATCAGGATTGCTTCTGTGAACCCCATCCT
  GGACCCCTGGATTTACATCCTTCTTCGGAAGACTGTGCTCAGTAAAGCCATAGAGAAGATCAAGTGCCTC
  TTCTGCCGCATTGGCGGTTCCGGCAGAGACAGCTCGGCCCAGCACTGCTCAGAGAGTCGGAGGACATCTT
  CCGCCATGTCCGGCCACTCTCGCTCCTTCCTCGCCCGGGAGTTAAAGGAGATCAGCAGCACGTCCCAGAC
  CCTCCTGTACCTGCCAGACCTGACTGAAAGCAGCCTCGGAGGCAGGAATTTGCTTCCAGGTTCGCATGGC
  ATGGGCCTGACCCAAGCAGACACCACCTCGCTGAGAACTTTGCGAATTTCCGAGACCTCAGACTCCTCCC
  AGGGCCAGGACTCTGAGAGTGTCCTGTTGGTGGATGAGGTTAGTGGGAGCCACAGAGAGGAGCCTGCCTC
  TAAAGGAAACTCTCTGCAAGTCACATTCCCCAGTGAAACTCTGAAATTATCTGAAAAATGTATA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226255 representing NM_001136079
  Red=Cloning site Green=Tags(s)
  
  MSIPGVNASFSSTPERLNSPVTIPAVMFIFGVVGNLVAIVVLCKSRKEQKETTFYTLVCGLAVTDLLGTL
  LVSPVTIATYMKGQWPGDQALCDYSTFILLFFGLSGLSIICAMSIERYLAINHAYFYSHYVDKRLAGLTL
  FAIYASNVLFCALPNMGLGRSERQYPGTWCFIDWTTNVTAYAAFSYMYAGFSSFLILATVLCNVLVCGAL
  LRMHRQFMRRTSLGTEQHHAAAAAAVASVACRGHAGASPALQRLSDFRRRRSFRRIAGAEIQMVILLIAT
  SLVVLICSIPLVVRVFINQLYQPNVVKDISRNPDLQAIRIASVNPILDPWIYILLRKTVLSKAIEKIKCL
  FCRIGGSGRDSSAQHCSESRRTSSAMSGHSRSFLARELKEISSTSQTLLYLPDLTESSLGGRNLLPGSHG
  MGLTQADTTSLRTLRISETSDSSQGQDSESVLLVDEVSGSHREEPASKGNSLQVTFPSETLKLSEKCI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136079
• ORF Size: 1464 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136079.2
• RefSeq Size: 3327 bp
• RefSeq ORF: 1467 bp
• Locus ID: 19219
• Cytogenetics: 15 1.99 cM
• Gene Summary: Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.[UniProtKB/Swiss-Prot Function]