Ddrgk1 (NM_029832) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG226240
Ddrgk1 (tGFP-tagged) - Mouse DDRGK domain containing 1 (Ddrgk1), (10ug)
  $500.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ddrgk1
Synonyms 1110001I20Rik; 2600009E05Rik; AI326138; Ufbp1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226240 representing NM_029832
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGGGCCCTGGGTGTATCTGGTGGCGGCAGTTTTGCTCATCGGCCTGATCCTCTTCCTGACTCGCA
GCCGGGGTCGGGCGGCAGCAGCTGACGGAGAACCACTGCACAATGAGGAAGAGAGGGCAGGAGCAGGCCA
GGTAGGCCGCTCTTTGCCCCAGGAGTCTGAAGAACAGAGAACTGGAAGCAGACCCCGGCGTCGGAGGGAC
TTGGGCAGCCGTCTACAGGCCCAGCGTCGAGCCCAGCGAGTGGCCTGGGAAGACGGGGATGAGAATGTGG
GTCAAACTGTTATTCCAGCCCAGGAGGAAGAAGGCATTGAGAAGCCAGCAGAAGTTCACCCAACAGGGAA
AATTGGAGCCAAGAAACTACGGAAGCTAGAGGAAAAACAGGCTCGAAAGGCTCAGCGAGAGGCAGAGGAG
GCTGAACGTGAAGAACGGAAACGCCTAGAGTCCCAACGTGAGGCCGAATGGAAGAAGGAAGAGGAACGGC
TTCGCCTGAAGGAAGAACAGAAGGAGGAGGAAGAGAGGAAGGCTCAGGAGGAGCAGGCCCGGCGGGAGCA
CGAGGAGTACCTGAAACTGAAGGAGGCCTTCGTGGTAGAAGAAGAAGGTGTTAGCGAAACCATGACTGAG
GAGCAGTCTCACAGCTTCCTGACAGAATTCATCAATTACATCAAGAAGTCCAAGGTTGTGCTTTTGGAAG
ATCTGGCTTTCCAGATGGGCCTAAGGACTCAGGACGCCATAAACCGCATCCAGGACCTGCTGACGGAGGG
GACTCTAACAGGTGTGATTGACGACCGGGGCAAGTTTATCTACATAACCCCAGAGGAACTGGCTGCCGTG
GCCAATTTCATCCGACAGCGGGGCCGGGTGTCCATCACAGAGCTTGCCCAGGCCAGCAACTCCCTCATCT
CCTGGGGCCAGGACCTCCCTGCCCAGGCTTCAGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226240 representing NM_029832
Red=Cloning site Green=Tags(s)

MVGPWVYLVAAVLLIGLILFLTRSRGRAAAADGEPLHNEEERAGAGQVGRSLPQESEEQRTGSRPRRRRD
LGSRLQAQRRAQRVAWEDGDENVGQTVIPAQEEEGIEKPAEVHPTGKIGAKKLRKLEEKQARKAQREAEE
AEREERKRLESQREAEWKKEEERLRLKEEQKEEEERKAQEEQARREHEEYLKLKEAFVVEEEGVSETMTE
EQSHSFLTEFINYIKKSKVVLLEDLAFQMGLRTQDAINRIQDLLTEGTLTGVIDDRGKFIYITPEELAAV
ANFIRQRGRVSITELAQASNSLISWGQDLPAQASA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029832
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029832.2, NP_084108.1
RefSeq Size 1101 bp
RefSeq ORF 948 bp
Locus ID 77006
UniProt ID Q80WW9
Cytogenetics 2 F1
Summary Protein which interacts with the E3 UFM1-protein ligase UFL1 and one of its substrates TRIP4 and is required for TRIP4 ufmylation. Through TRIP4 ufmylation may regulate nuclear receptors-mediated transcription. May play a role in NF-kappa-B-mediated transcription through regulation of the phosphorylation and the degradation of NFKBIA, the inhibitor of NF-kappa-B (By similarity). May also play a role in the cellular response to endoplasmic reticulum stress (PubMed:21494687). Plays a role in cartilage development through SOX9, inhibiting the ubiquitin-mediated proteasomal degradation of this transcriptional regulator (PubMed:28263186).UniProtKB/Swiss-Prot Function
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"NM_029832" in other vectors (4)
SKU Description Size Price
MC211802 Ddrgk1 (untagged) - Mouse DDRGK domain containing 1 (Ddrgk1), (10ug) 10 ug
$330.00
MR226240 Ddrgk1 (Myc-DDK-tagged) - Mouse DDRGK domain containing 1 (Ddrgk1) 10 ug
$289.00 $300.00
MR226240L3 Lenti ORF clone of Ddrgk1 (Myc-DDK-tagged) - Mouse DDRGK domain containing 1 (Ddrgk1) 10 ug
$600.00
MR226240L4 Lenti ORF clone of Ddrgk1 (mGFP-tagged) - Mouse DDRGK domain containing 1 (Ddrgk1) 10 ug
$600.00

Plasmid Map for MG226240

Download sequence for

Cloning scheme for MG226240

Circular map for MG226240

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