Itgal (NM_008400) Mouse Tagged ORF Clone

CAT#: MG226164

Itgal (tGFP-tagged) - Mouse integrin alpha L (Itgal), (10ug)

Product Images

Circular map for MG226164

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itgal
• Synonyms: (p180); Cd11a; LFA-1; LFA-1A; Ly-15; Ly-21
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226164 representing NM_008400
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTTCCGGATTGCGGGCCCCAGACTTTTGCTACTGGGACTCCAGCTGTTTGCCAAGGCCTGGAGCT
  ACAACCTGGACACACGGCCTACGCAGAGCTTCTTGGCACAAGCTGGAAGACATTTTGGGTACCAGGTCTT
  GCAGATTGAAGATGGGGTTGTCGTGGGAGCCCCAGGTGAGGGGGACAACACGGGAGGCCTCTATCACTGC
  CGAACAAGCAGCGAGTTCTGCCAGCCAGTCAGCCTACATGGTTCTAACCATACCTCCAAGTACTTGGGAA
  TGACGCTGGCAACAGATGCCGCCAAGGGAAGCCTTTTGGCCTGTGACCCTGGACTGTCTCGGACATGCGA
  TCAGAACACTTACCTCAGTGGCCTCTGCTACCTCTTCCCCCAGAGTCTGGAGGGACCTATGTTACAAAAT
  CGTCCCGCCTATCAGGAATGTATGAAGGGCAAAGTCGACCTGGTGTTTCTGTTCGATGGCTCACAGAGCT
  TGGATAGAAAGGACTTTGAAAAAATCCTGGAATTCATGAAGGATGTGATGAGGAAGCTCAGCAATACTTC
  CTACCAGTTTGCTGCCGTCCAGTTCTCCACAGACTGCAGAACAGAATTTACTTTCTTGGACTACGTTAAG
  CAGAACAAGAACCCCGATGTTCTGCTAGGCAGCGTGCAGCCCATGTTCTTGCTGACCAATACCTTTCGTG
  CCATCAACTATGTGGTGGCACACGTGTTCAAAGAAGAGTCTGGTGCCAGGCCGGATGCTACCAAGGTGCT
  TGTCATCATTACAGACGGGGAGGCAAGTGATAAAGGCAACATCAGTGCGGCCCACGACATAACCCGCTAC
  ATCATCGGGATTGGCAAGCATTTTGTGAGCGTACAAAAGCAAAAGACGCTCCACATATTTGCCTCAGAAC
  CTGTAGAGGAATTTGTGAAGATTCTGGACACCTTTGAGAAGCTGAAGGATCTTTTTACTGACCTGCAGAG
  GAGGATTTATGCTATTGAGGGCACAAACAGACAGGACCTGACATCCTTTAACATGGAACTCTCCTCCAGC
  GGGATCAGCGCAGACCTCAGCAAGGGCCATGCAGTTGTGGGAGCTGTTGGGGCTAAGGATTGGGCCGGGG
  GCTTTCTGGACCTGCGTGAAGACCTGCAGGGTGCCACATTTGTTGGGCAGGAACCGCTGACCTCAGATGT
  GAGAGGGGGCTACCTGGGTTACACTGTGGCCTGGATGACCTCCCGGAGCTCCAGACCCCTGCTGGCAGCA
  GGAGCCCCACGGTACCAGCATGTGGGACAAGTACTGCTTTTCCAAGCCCCAGAGGCTGGAGGACGTTGGA
  ACCAAACCCAGAAGATAGAAGGGACTCAGATCGGATCTTACTTTGGTGGGGAACTATGTAGTGTTGACCT
  GGACCAAGATGGCGAGGCAGAGCTGCTGCTGATTGGAGCACCCCTGTTCTTTGGGGAGCAGAGAGGAGGC
  CGAGTGTTCACTTACCAGAGAAGACAGTCGCTGTTTGAAATGGTCTCAGAGCTACAGGGTGACCCTGGCT
  ACCCGCTTGGTCGGTTTGGAGCCGCCATAACTGCCCTGACGGACATCAATGGGGATAGGCTGACTGATGT
  GGCTGTGGGAGCCCCTTTGGAGGAGCAGGGTGCTGTGTACATCTTCAATGGGAAGCCTGGTGGGCTCAGT
  CCCCAGCCAAGCCAGCGTATACAAGGAGCCCAGGTGTTCCCAGGAATCCGGTGGTTTGGCCGCTCCATCC
  ATGGGGTGAAGGACCTTGGAGGGGACAGGCTGGCAGATGTGGTTGTAGGAGCTGAGGGTCGGGTGGTTGT
  GCTGAGCTCCAGGCCGGTGGTGGATGTGGTCACTGAGCTGTCGTTCTCCCCAGAGGAAATCCCAGTGCAC
  GAGGTGGAGTGCTCCTACTCAGCCAGGGAGGAGCAGAAACACGGAGTCAAGCTCAAGGCATGCTTCCGGA
  TCAAGCCCCTCACGCCACAGTTTCAAGGTCGCCTGCTTGCCAACCTCAGCTACACCCTGCAGCTGGATGG
  CCATCGGATGAGGAGCCGAGGGTTGTTCCCAGATGGAAGCCACGAGCTCAGTGGAAACACCTCCATCACC
  CCAGATAAATCCTGCTTGGACTTCCACTTCCACTTCCCGATCTGCATTCAAGACCTCATCTCCCCTATCA
  ATGTCTCCCTGAATTTCTCTCTTTTGGAGGAAGAAGGAACACCAAGGGACCAAAAGGTGGGCAGGGCCAT
  GCAGCCTATCCTGAGACCTTCAATCCACACAGTGACTAAGGAGATCCCTTTTGAGAAGAACTGTGGTGAA
  GATAAGAAGTGTGAGGCAAACCTGACCCTGTCATCCCCTGCCAGATCTGGACCCCTGCGTCTGATGTCCT
  CTGCCAGCCTTGCTGTGGAGTGGACACTGAGCAACTCAGGGGAAGATGCCTACTGGGTGCGATTAGACCT
  GGACTTCCCTCGGGGACTCTCCTTCCGGAAAGTGGAGATGCTTCAGCCACACAGCCGAATGCCTGTGAGC
  TGCGAGGAGCTCACCGAGGGGTCAAGTCTCCTGACTAAGACACTGAAATGCAATGTAAGCTCTCCCATCT
  TCAAAGCAGGCCAGGAGGTGAGCCTCCAGGTGATGTTTAACACGCTACTCAACAGCTCCTGGGAAGACTT
  CGTCGAGCTGAATGGCACTGTGCACTGTGAGAATGAGAACTCAAGCCTCCAGGAGGACAACTCAGCCGCC
  ACCCACATTCCTGTCCTGTACCCTGTCAACATCCTTACTAAGGAGCAGGAGAACTCCACCCTCTATATCA
  GTTTCACCCCTAAAGGTCCCAAGACCCAACAAGTCCAGCATGTCTACCAGGTGAGGATTCAGCCATCTGC
  CTATGACCACAACATGCCCACACTAGAGGCCTTGGTTGGGGTGCCCCGGCCTCACAGTGAGGACCTCATC
  ACATACACATGGAGTGTACAGACGGATCCCCTTGTCACTTGCCACAGCGAGGACCTGAAGAGGCCGTCCA
  GCGAAGCTGAGCAGCCTTGTCTGCCTGGAGTCCAGTTCCGCTGTCCAATTGTCTTCAGGCGGGAGATCCT
  CATCCAAGTGACGGGGACCGTGGAACTCTCCAAGGAAATCAAGGCCTCCTCCACACTCAGCCTCTGCAGC
  TCACTCTCCGTCTCCTTCAACAGCAGCAAGCATTTCCATTTGTATGGCAGCAAAGCCTCTGAGGCCCAGG
  TCCTCGTGAAGGTTGACCTGATCCACGAGAAGGAGATGCTTCACGTGTACGTGCTCAGCGGCATTGGGGG
  CCTCGTGCTTCTGTTCCTGATTTTCCTGGCGCTCTACAAGGTTGGCTTCTTCAAACGGAACCTGAAGGAG
  AAGATGGAGGCTGATGGAGGTGTTCCAAATGGAAGCCCTCCAGAAGACACTGACCCTCTGGCAGTACCTG
  GGGAAGAGACCAAAGATATGGGCTGTCTAGAGCCCCTCCGGGAGAGTGACAAGGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226164 representing NM_008400
  Red=Cloning site Green=Tags(s)
  
  MSFRIAGPRLLLLGLQLFAKAWSYNLDTRPTQSFLAQAGRHFGYQVLQIEDGVVVGAPGEGDNTGGLYHC
  RTSSEFCQPVSLHGSNHTSKYLGMTLATDAAKGSLLACDPGLSRTCDQNTYLSGLCYLFPQSLEGPMLQN
  RPAYQECMKGKVDLVFLFDGSQSLDRKDFEKILEFMKDVMRKLSNTSYQFAAVQFSTDCRTEFTFLDYVK
  QNKNPDVLLGSVQPMFLLTNTFRAINYVVAHVFKEESGARPDATKVLVIITDGEASDKGNISAAHDITRY
  IIGIGKHFVSVQKQKTLHIFASEPVEEFVKILDTFEKLKDLFTDLQRRIYAIEGTNRQDLTSFNMELSSS
  GISADLSKGHAVVGAVGAKDWAGGFLDLREDLQGATFVGQEPLTSDVRGGYLGYTVAWMTSRSSRPLLAA
  GAPRYQHVGQVLLFQAPEAGGRWNQTQKIEGTQIGSYFGGELCSVDLDQDGEAELLLIGAPLFFGEQRGG
  RVFTYQRRQSLFEMVSELQGDPGYPLGRFGAAITALTDINGDRLTDVAVGAPLEEQGAVYIFNGKPGGLS
  PQPSQRIQGAQVFPGIRWFGRSIHGVKDLGGDRLADVVVGAEGRVVVLSSRPVVDVVTELSFSPEEIPVH
  EVECSYSAREEQKHGVKLKACFRIKPLTPQFQGRLLANLSYTLQLDGHRMRSRGLFPDGSHELSGNTSIT
  PDKSCLDFHFHFPICIQDLISPINVSLNFSLLEEEGTPRDQKVGRAMQPILRPSIHTVTKEIPFEKNCGE
  DKKCEANLTLSSPARSGPLRLMSSASLAVEWTLSNSGEDAYWVRLDLDFPRGLSFRKVEMLQPHSRMPVS
  CEELTEGSSLLTKTLKCNVSSPIFKAGQEVSLQVMFNTLLNSSWEDFVELNGTVHCENENSSLQEDNSAA
  THIPVLYPVNILTKEQENSTLYISFTPKGPKTQQVQHVYQVRIQPSAYDHNMPTLEALVGVPRPHSEDLI
  TYTWSVQTDPLVTCHSEDLKRPSSEAEQPCLPGVQFRCPIVFRREILIQVTGTVELSKEIKASSTLSLCS
  SLSVSFNSSKHFHLYGSKASEAQVLVKVDLIHEKEMLHVYVLSGIGGLVLLFLIFLALYKVGFFKRNLKE
  KMEADGGVPNGSPPEDTDPLAVPGEETKDMGCLEPLRESDKD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008400
• ORF Size: 3480 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008400.2, NP_032426.2
• RefSeq Size: 3892 bp
• RefSeq ORF: 3483 bp
• Locus ID: 16408
• Cytogenetics: 7 69.44 cM