Dll1 (NM_007865) Mouse Tagged ORF Clone

CAT#: MG226161

Dll1 (tGFP-tagged) - Mouse delta-like 1 (Drosophila) (Dll1), (10ug)

Product Images

Circular map for MG226161

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dll1
• Synonyms: Delta1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226161 representing NM_007865
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGTCGGAGCGCGCTAGCCCTTGCCGTGGTCTCTGCCCTGCTGTGCCAGGTCTGGAGCTCCGGCG
  TATTTGAGCTGAAGCTGCAGGAGTTCGTCAACAAGAAGGGGCTGCTGGGGAACCGCAACTGCTGCCGCGG
  GGGCTCTGGCCCGCCTTGCGCCTGCAGGACCTTCTTTCGCGTATGCCTCAAGCACTACCAGGCCAGCGTG
  TCACCGGAGCCACCCTGCACCTACGGCAGTGCTGTCACGCCAGTGCTGGGTGTCGACTCCTTCAGCCTGC
  CTGATGGCGCAGGCATCGACCCCGCCTTCAGCAACCCCATCCGATTCCCCTTCGGCTTCACCTGGCCAGG
  TACCTTCTCTCTGATCATTGAAGCCCTCCATACAGACTCTCCCGATGACCTCGCAACAGAAAACCCAGAA
  AGACTCATCAGCCGCCTGACCACACAGAGGCACCTCACTGTGGGAGAAGAATGGTCTCAGGACCTTCACA
  GTAGCGGCCGCACAGACCTCCGGTACTCTTACCGGTTTGTGTGTGACGAGCACTACTACGGAGAAGGTTG
  CTCTGTGTTCTGCCGACCTCGGGATGACGCCTTTGGCCACTTCACCTGCGGGGACAGAGGGGAGAAGATG
  TGCGACCCTGGCTGGAAAGGCCAGTACTGCACTGACCCAATCTGTCTGCCAGGGTGTGATGACCAACATG
  GATACTGTGACAAACCAGGGGAGTGCAAGTGCAGAGTTGGCTGGCAGGGCCGCTACTGCGATGAGTGCAT
  CCGATACCCAGGTTGTCTCCATGGCACCTGCCAGCAACCCTGGCAGTGTAACTGCCAGGAAGGCTGGGGG
  GGCCTTTTCTGCAACCAAGACCTGAACTACTGTACTCACCATAAGCCGTGCAGGAATGGAGCCACCTGCA
  CCAACACGGGCCAGGGGAGCTACACATGTTCCTGCCGACCTGGGTATACAGGTGCCAACTGTGAGCTGGA
  AGTAGATGAGTGTGCTCCTAGCCCCTGCAAGAACGGAGCGAGCTGCACGGACCTTGAGGACAGCTTCTCT
  TGCACCTGCCCTCCCGGCTTCTATGGCAAGGTCTGTGAGCTGAGCGCCATGACCTGTGCAGATGGCCCTT
  GCTTCAATGGAGGACGATGTTCAGATAACCCTGACGGAGGCTACACCTGCCATTGCCCCTTGGGCTTCTC
  TGGCTTCAACTGTGAGAAGAAGATGGATCTCTGCGGCTCTTCCCCTTGTTCTAACGGTGCCAAGTGTGTG
  GACCTCGGCAACTCTTACCTGTGCCGGTGCCAGGCTGGCTTCTCCGGGAGGTACTGCGAGGACAATGTGG
  ATGACTGTGCCTCCTCCCCGTGTGCAAATGGGGGCACCTGCCGGGACAGTGTGAACGACTTCTCCTGTAC
  CTGCCCACCTGGCTACACGGGCAAGAACTGCAGCGCCCCTGTCAGCAGGTGTGAGCATGCACCCTGCCAT
  AATGGGGCCACCTGCCACCAGAGGGGCCAGCGCTACATGTGTGAGTGCGCCCAGGGCTATGGCGGCCCCA
  ACTGCCAGTTTCTGCTCCCTGAGCCACCACCAGGGCCCATGGTGGTGGACCTCAGTGAGAGGCATATGGA
  GAGCCAGGGCGGGCCCTTCCCCTGGGTGGCCGTGTGTGCCGGGGTGGTGCTTGTCCTCCTGCTGCTGCTG
  GGCTGTGCTGCTGTGGTGGTCTGCGTCCGGCTGAAGCTACAGAAACACCAGCCTCCACCTGAACCCTGTG
  GGGGAGAGACAGAAACCATGAACAACCTAGCCAATTGCCAGCGCGAGAAGGACGTTTCTGTTAGCATCAT
  TGGGGCTACCCAGATCAAGAACACCAACAAGAAGGCGGACTTTCACGGGGACCATGGAGCCGAGAAGAGC
  AGCTTTAAGGTCCGATACCCCACTGTGGACTATAACCTCGTTCGAGACCTCAAGGGAGATGAAGCCACGG
  TCAGGGATACACACAGCAAACGTGACACCAAGTGCCAGTCACAGAGCTCTGCAGGAGAAGAGAAGATCGC
  CCCAACACTTAGGGGTGGGGAGATTCCTGACAGAAAAAGGCCAGAGTCTGTCTACTCTACTTCAAAGGAC
  ACCAAGTACCAGTCGGTGTATGTTCTGTCTGCAGAAAAGGATGAGTGTGTTATAGCGACTGAGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226161 representing NM_007865
  Red=Cloning site Green=Tags(s)
  
  MGRRSALALAVVSALLCQVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASV
  SPEPPCTYGSAVTPVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPE
  RLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGDRGEKM
  CDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWG
  GLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCKNGASCTDLEDSFS
  CTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPLGFSGFNCEKKMDLCGSSPCSNGAKCV
  DLGNSYLCRCQAGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGKNCSAPVSRCEHAPCH
  NGATCHQRGQRYMCECAQGYGGPNCQFLLPEPPPGPMVVDLSERHMESQGGPFPWVAVCAGVVLVLLLLL
  GCAAVVVCVRLKLQKHQPPPEPCGGETETMNNLANCQREKDVSVSIIGATQIKNTNKKADFHGDHGAEKS
  SFKVRYPTVDYNLVRDLKGDEATVRDTHSKRDTKCQSQSSAGEEKIAPTLRGGEIPDRKRPESVYSTSKD
  TKYQSVYVLSAEKDECVIATEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007865
• ORF Size: 2166 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007865.3, NP_031891.2
• RefSeq Size: 3444 bp
• RefSeq ORF: 2169 bp
• Locus ID: 13388
• UniProt ID: Q61483
• Cytogenetics: 17 8.95 cM
• Gene Summary: Transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner (PubMed:21985982, PubMed:10958687). Following transinteraction, ligand cells produce mechanical force that depends of a clathrin-mediated endocytosis, requiring ligand ubiquitination, EPN1 interaction, and actin polymerisation; these events promote Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling through induction of cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD) (PubMed:10958687, PubMed:18676613). Is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme; the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation (PubMed:17194759, PubMed:19562077, PubMed:18997111, PubMed:23695674, PubMed:16495313, PubMed:21238454, PubMed:22282195, PubMed:7671806, PubMed:17960184, PubMed:22529374, PubMed:19389377, PubMed:23699523, PubMed:19144989, PubMed:23688253, PubMed:23806616, PubMed:26114479, PubMed:22940113, PubMed:25220152, PubMed:20081190, PubMed:21572390, PubMed:22096075). Plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction, most likely through the lateral inhibitory system in an endogenous level dependent-manner (PubMed:7671806, PubMed:18997111). During neocortex development, Dll1-Notch signaling transmission is mediated by dynamic interactions between intermediate neurogenic progenitors and radial glia; the cell-cell interactions are mediated via dynamic and transient elongation processes, likely to reactivate/maintain Notch activity in neighboring progenitors, and coordinate progenitor cell division and differentiation across radial and zonal boundaries (PubMed:23699523). During cerebellar development, regulates Bergmann glial monolayer formation and its morphological maturation through a Notch signaling pathway (PubMed:23688253). At the retina and spinal cord level, regulates neurogenesis by preventing the premature differentiation of neural progenitors and also by maintaining progenitors in spinal cord through Notch signaling pathway (PubMed:19389377, PubMed:26114479). Also controls neurogenesis of the neural tube in a progenitor domain-specific fashion along the dorsoventral axis (PubMed:20081190). Maintains quiescence of neural stem cells and plays a role as a fate determinant that segregates asymmetrically to one daughter cell during neural stem cells mitosis, resulting in neuronal differentiation in Dll1-inheriting cell (PubMed:23695674). Plays a role in immune systeme development, namely the development of all T-cells and marginal zone (MZ) B cells (PubMed:15146182, PubMed:19217325). Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor (By similarity). Upon MMP14 cleavage, negatively regulates Notch signaling in haematopoietic progenitor cells to specifically maintain normal B-cell development in bone marrow (PubMed:21572390). Also plays a role during muscle development. During early development, inhibits myoblasts differentiation from the medial dermomyotomal lip and later regulates progenitor cell differentiation (PubMed:17194759). Directly modulates cell adhesion and basal lamina formation in satellite cells through Notch signaling. Maintains myogenic progenitors pool by suppressing differentiation through down-regulation of MYOD1 and is required for satellite cell homing and PAX7 expression (PubMed:22940113). During craniofacial and trunk myogenesis suppresses differentiation of cranial mesoderm-derived and somite-derived muscle via MYOD1 regulation but in cranial mes