Cpa3 (NM_007753) Mouse Tagged ORF Clone

CAT#: MG226143

Cpa3 (tGFP-tagged) - Mouse carboxypeptidase A3 mast cell (Cpa3), (10ug)

Product Images

Circular map for MG226143

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cpa3
• Synonyms: MC-C; MC-CPA; mMC-C
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226143 representing NM_007753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTTCTTCTTGCTTATGGCTGTCATCTACACTACACTTGCAATTGCTCCTGTCCACTTTGACAGGG
  AGAAGGTATTCCGTGTGAAACTCCAGAATGAAAAGCACGCCAGTGTCTTGAAGAACTTGACCCAAAGCAT
  TGAGCTCGACTTCTGGTATCCAGATGCTATCCATGATATTGCTGTGAATATGACTGTGGATTTCCGAGTT
  AGTGAGAAGGAATCTCAGACCATCCAGTCAACCTTGGAACAACATAAAATCCATTATGAAATTCTGATAC
  ATGACCTACAAGAAGAGATAGAGAAACAGTTTGATGTGAAAGATGAAATCGCAGGCAGGCACAGTTATGC
  AAAATACAATGACTGGGACAAGATTGTTTCTTGGACTGAAAAAATGTTAGAAAAACATCCTGAGATGGTT
  TCAAGGATTAAAATTGGATCAACTGTTGAAGATAATCCATTATATGTCCTCAAGATTGGGAAAAAAGATG
  GAGAAAGAAAGGCTATCTTTATGGACTGTGGCATTCATGCACGTGAATGGATTTCTCCAGCTTTCTGTCA
  GTGGTTTGTCTATCAGGCAACCAAGAGTTATGGAAAAAATAAAATCATGACCAAACTCTTGGACCGGATG
  AATTTTTACGTTCTGCCTGTATTCAATGTAGACGGATACATTTGGTCATGGACACAGGATCGAATGTGGA
  GAAAAAACCGTTCCAGGAACCAAAACTCCACCTGCATTGGCACTGACCTCAACAGGAACTTTGATGTCTC
  GTGGGACTCTTCTCCAAACACCAACAAACCATGCCTCAATGTCTACAGGGGACCTGCACCAGAGTCTGAG
  AAAGAGACAAAAGCTGTCACCAACTTCATTCGAAGCCATCTGAACTCAATCAAGGCTTATATCACTTTCC
  ATTCCTACTCGCAGATGCTATTAATTCCTTATGGCTACACATTCAAACTGCCTCCTAACCACCAGGACCT
  ATTGAAAGTTGCAAGGATTGCCACGGATGCTCTCTCAACTAGATATGAAACCCGTTACATCTACGGCCCA
  ATAGCATCCACAATTTACAAGACTTCAGGCTCTTCCTTAGACTGGGTTTATGACCTGGGCATCAAACACA
  CATTTGCCTTTGAGCTTCGTGATAAAGGAAAGTCTGGGTTTCTTCTTCCAGAATCCCGGATAAAGCCAAC
  ATGTAAAGAAACCATGCTTTCTGTCAAATTTATTGCCAAGTATATCCTCAAGAATACTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226143 representing NM_007753
  Red=Cloning site Green=Tags(s)
  
  MRFFLLMAVIYTTLAIAPVHFDREKVFRVKLQNEKHASVLKNLTQSIELDFWYPDAIHDIAVNMTVDFRV
  SEKESQTIQSTLEQHKIHYEILIHDLQEEIEKQFDVKDEIAGRHSYAKYNDWDKIVSWTEKMLEKHPEMV
  SRIKIGSTVEDNPLYVLKIGKKDGERKAIFMDCGIHAREWISPAFCQWFVYQATKSYGKNKIMTKLLDRM
  NFYVLPVFNVDGYIWSWTQDRMWRKNRSRNQNSTCIGTDLNRNFDVSWDSSPNTNKPCLNVYRGPAPESE
  KETKAVTNFIRSHLNSIKAYITFHSYSQMLLIPYGYTFKLPPNHQDLLKVARIATDALSTRYETRYIYGP
  IASTIYKTSGSSLDWVYDLGIKHTFAFELRDKGKSGFLLPESRIKPTCKETMLSVKFIAKYILKNTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007753
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007753.2, NP_031779.1
• RefSeq Size: 1458 bp
• RefSeq ORF: 1254 bp
• Locus ID: 12873
• UniProt ID: P15089
• Cytogenetics: 3 6.25 cM
• Gene Summary: This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases and preproprotein that is proteolytically processed to generate a mature protein product. This product is released by mast cells and may be involved in the degradation of endogenous proteins and the inactivation of venom-associated peptides. Homozygous knockout mice for this gene exhibit impaired mast cell development. [provided by RefSeq, Aug 2015]