Oprm1 (NM_001039652) Mouse Tagged ORF Clone

CAT#: MG226134

Oprm1 (tGFP-tagged) - Mouse opioid receptor mu 1 (Oprm1), (10ug)

Product Images

Circular map for MG226134

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Oprm1
• Synonyms: m; M-OR-1; MO; MOP-R; mor; MOR-1; MOR-1O; mu; muOR; O; Oprm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG226134 representing NM_001039652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGCAGCGCCGGCCCAGGGAACATCAGCGACTGCTCTGACCCCTTAGCTCCTGCAAGTTGCTCCC
  CAGCACCTGGCTCCTGGCTCAACTTGTCCCACGTTGATGGCAACCAGTCCGACCCATGCGGTCCTAACCG
  CACGGGGCTTGGCGGGAGCCACAGCCTGTGCCCTCAGACCGGCAGCCCTTCCATGGTCACAGCCATCACC
  ATCATGGCCCTCTATTCTATCGTGTGTGTAGTGGGCCTCTTTGGAAACTTCCTGGTCATGTATGTGATTG
  TAAGATATACCAAAATGAAGACTGCCACCAACATCTACATTTTCAACCTTGCTCTGGCAGATGCCTTAGC
  CACTAGCACGCTGCCCTTTCAGAGTGTTAACTACCTGATGGGAACGTGGCCCTTTGGAAACATCCTCTGC
  AAGATCGTGATCTCAATAGACTACTACAACATGTTCACCAGTATCTTCACCCTCTGCACCATGAGTGTAG
  ACCGCTACATTGCCGTCTGCCACCCGGTCAAGGCCCTGGATTTCCGTACCCCCCGAAATGCCAAAATTGT
  CAATGTCTGCAACTGGATCCTCTCTTCTGCCATTGGTCTGCCCGTAATGTTCATGGCAACCACAAAATAC
  AGGCAGGGGTCCATAGATTGCACCCTCACGTTCTCTCATCCCACATGGTACTGGGAGAACCTGCTCAAAA
  TCTGTGTCTTCATCTTCGCCTTCATCATGCCGGTCCTCATCATCACTGTGTGTTATGGACTGATGATCTT
  ACGACTCAAGAGTGTCCGCATGCTGTCGGGCTCCAAAGAAAAGGACAGGAACCTGCGCAGGATCACCCGG
  ATGGTGCTGGTGGTCGTGGCTGTATTTATTGTCTGCTGGACCCCCATCCACATCTATGTCATCATCAAAG
  CACTGATCACGATTCCAGAAACCACTTTCCAGACTGTTTCCTGGCACTTCTGCATTGCCTTGGGTTACAC
  AAACAGCTGCCTGAACCCAGTTCTTTATGCGTTCCTGGATGAAAACTTCAAACGATGTTTTAGAGAGTTC
  TGCATCCCAACTTCCTCCACAATCGAACAGCAAAACTCTGCTCGAATCCGTCAAAACACTAGGGAACACC
  CCTCCACGGCTAATACAGTGGATCGAACTAACCACCAGCCAACCCTGGCAGTCAGCGTGGCCCAGATCTT
  TACAGGATATCCTTCTCCGACTCATGTTGAAAAACCCTGCAAGAGTTGCATGGACAGAGGAATGAGGAAC
  CTTCTTCCTGATGATGGCCCAAGACAGGAATCCGGGGAAGGCCAGCTTGGCAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG226134 representing NM_001039652
  Red=Cloning site Green=Tags(s)
  
  MDSSAGPGNISDCSDPLAPASCSPAPGSWLNLSHVDGNQSDPCGPNRTGLGGSHSLCPQTGSPSMVTAIT
  IMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLPFQSVNYLMGTWPFGNILC
  KIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPRNAKIVNVCNWILSSAIGLPVMFMATTKY
  RQGSIDCTLTFSHPTWYWENLLKICVFIFAFIMPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITR
  MVLVVVAVFIVCWTPIHIYVIIKALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREF
  CIPTSSTIEQQNSARIRQNTREHPSTANTVDRTNHQPTLAVSVAQIFTGYPSPTHVEKPCKSCMDRGMRN
  LLPDDGPRQESGEGQLGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039652
• ORF Size: 1314 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039652.2, NP_001034741.1
• RefSeq Size: 2641 bp
• RefSeq ORF: 1317 bp
• Locus ID: 18390
• UniProt ID: P42866
• Cytogenetics: 10 1.85 cM
• Gene Summary: This gene encodes the mu opioid receptor which is where drugs such as morphine and other opioids have pharmacological effects. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]