Ric3 (NM_001038624) Mouse Tagged ORF Clone

CAT#: MG225825

Ric3 (tGFP-tagged) - Mouse resistance to inhibitors of cholinesterase 3 homolog (C. elegans) (Ric3) transcript variant 1, (10ug)

Product Images

Circular map for MG225825

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ric3
• Synonyms: E130307J04Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225825 representing NM_001038624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTACTCCACGGTGCAGAGAGTGGCGCTGGCCTCGGGGCTCGTCCTGGCCGTGTCGCTGCTGCTGC
  CCAAGGCCTTCTTGTCTCGCGGGAAGCGACCGGAGCCGCCGCCGGGCCCGGAAGGAAAATTGGACCGATT
  TCCACCTATGATGCATCACCACTCGGCACCCTCAGATGGTCAGACACCAGGGGCTCGTTTCCAGAGGTCT
  CACCTTGCAGAGGCCTTTGCAAAGGCCAAGGGAGCAGGTGGAGGTGCTGGAGGAGGGGGTAGTGGAAGAG
  GACTGATGGGCCAGATCATTCCAATCTATGGCTTTGGGATCTTTTTGTACATACTGTACATTTTGTTTAA
  GCTTTCAAAGGGGAAAACTGCAGAGGATCGGAACTGCTCCACTGCCCCACCTGGAAACGCCCACAGGAAG
  ATTACCAACTTTGAGCTTGTTCAACTACAAGAAAAACTAAAAGAGACAGAAGAAGCCATGGAAAAATTAA
  TCAACAGAGTTGGACCTAATGGTGAGAGCAGAGCACAGGCTGTGACTTCTGACCAAGAGAAACGATTACT
  GCATCAGCTCCGAGAAATCACCAGGGTCATGAAAGAAGGCAAGTTCATCGACACATCTCCAGAGAAGGAA
  GCTGAGGAAGCCCCATACATGGAGGACTGGGAAGGTTACCCAGAAGAGACTTATCCAATATATGACCTTT
  CGGATGGTATCAAGCGTAGGCAAGAAACAATCCTGGTGGATTACCCTGACCTAAAAGAGCCCTCTGCTGA
  AGAAATTGCTGAACAAATGGGAGAGATAGAAGAGGAAGGATCAGAGCGTTTGAGCTGGGATCATTTGCCC
  ACTGACCCTGGAGCCCAGAAAGATAATTCTGTTGCCCCGTGTGATCCCAAACCAGAATCATGCTCCTGCT
  GTGTTCATGAAGAGGAGGATCCTGCTGTCTTGGCAGAGAATGCTGGTTTCAGTGCAGATGGCTACAGTGA
  GCAAGAGGAAGCCACCAAGGAAAACTTGCCCCAGGACTTTACAAATGAAGGGCTGGGAGTCAGCACTGAT
  AATGCACACGTGGGTGGTATGTTGAGGAAGCGCAACCCCCAGGGTTTTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225825 representing NM_001038624
  Red=Cloning site Green=Tags(s)
  
  MAYSTVQRVALASGLVLAVSLLLPKAFLSRGKRPEPPPGPEGKLDRFPPMMHHHSAPSDGQTPGARFQRS
  HLAEAFAKAKGAGGGAGGGGSGRGLMGQIIPIYGFGIFLYILYILFKLSKGKTAEDRNCSTAPPGNAHRK
  ITNFELVQLQEKLKETEEAMEKLINRVGPNGESRAQAVTSDQEKRLLHQLREITRVMKEGKFIDTSPEKE
  AEEAPYMEDWEGYPEETYPIYDLSDGIKRRQETILVDYPDLKEPSAEEIAEQMGEIEEEGSERLSWDHLP
  TDPGAQKDNSVAPCDPKPESCSCCVHEEEDPAVLAENAGFSADGYSEQEEATKENLPQDFTNEGLGVSTD
  NAHVGGMLRKRNPQGFE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001038624
• ORF Size: 1101 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038624.1, NP_001033713.1
• RefSeq Size: 5303 bp
• RefSeq ORF: 1104 bp
• Locus ID: 320360
• UniProt ID: Q8BPM6
• Cytogenetics: 7 E3
• Gene Summary: Promotes functional expression of homomeric alpha-7 and alpha-8 nicotinic acetylcholine receptors at the cell surface. May also promote functional expression of homomeric serotoninergic 5-HT3 receptors, and of heteromeric acetylcholine receptors alpha-3/beta-2, alpha-3/beta-4, alpha-4/beta-2 and alpha-4/beta-4.[UniProtKB/Swiss-Prot Function]