Ifnar2 (NM_001110498) Mouse Tagged ORF Clone

CAT#: MG225728

Ifnar2 (tGFP-tagged) - Mouse interferon (alpha and beta) receptor 2 (Ifnar2) transcript variant 2, (10ug)

Product Images

Circular map for MG225728

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ifnar2
• Synonyms: AI747302; Ifnar-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225728 representing NM_001110498
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTTCACGGTGCACCGTCTCTGCCGTCGGTCTCCTCAGCTTGTGTCTTGTGGTGTCTGCGAGCCTAG
  AGACTATCACACCGTCTGCTTTTGATGGGTATCCAGATGAACCTTGCACTATAAACATAACAATACGAAA
  TTCCCGGCTAATTTTATCCTGGGAATTAGAGAACAAGTCTGGCCCACCCGCTAACTACACCCTCTGGTAC
  ACAGTCATGAGCAAAGACGAAAATCTGACGAAGGTTAAGAACTGTTCAGATACCACGAAGTCATCATGTG
  ACGTGACAGATAAGTGGTTGGAGGGCATGGAGAGCTACGTCGTCGCCATCGTCATAGTGCACAGAGGGGA
  CTTGACCGTGTGCCGCTGCTCAGACTACATCGTGCCTGCAAACGCTCCTCTTGAGCCGCCAGAATTTGAG
  ATCGTTGGCTTTACAGACCACATAAACGTGACGATGGAATTTCCACCTGTCACTTCCAAAATAATCCAGG
  AAAAGATGAAGACTACACCCTTTGTCATCAAAGAACAGATAGGGGACAGCGTTAGGAAGAAGCACGAGCC
  CAAAGTGAATAATGTCACTGGGAACTTCACATTTGTCCTTAGAGACTTACTTCCAAAGACAAACTACTGT
  GTATCTCTTTATTTTGATGATGACCCCGCAATAAAATCTCCCTTAAAATGCATCGTCCTTCAGCCTGGCC
  AGGAATCAGGTATGGCTAGGTTTTTAAAATTTGCACTATTGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225728 representing NM_001110498
  Red=Cloning site Green=Tags(s)
  
  MRSRCTVSAVGLLSLCLVVSASLETITPSAFDGYPDEPCTINITIRNSRLILSWELENKSGPPANYTLWY
  TVMSKDENLTKVKNCSDTTKSSCDVTDKWLEGMESYVVAIVIVHRGDLTVCRCSDYIVPANAPLEPPEFE
  IVGFTDHINVTMEFPPVTSKIIQEKMKTTPFVIKEQIGDSVRKKHEPKVNNVTGNFTFVLRDLLPKTNYC
  VSLYFDDDPAIKSPLKCIVLQPGQESGMARFLKFALLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001110498
• ORF Size: 744 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110498.1, NP_001103968.1
• RefSeq Size: 1169 bp
• RefSeq ORF: 747 bp
• Locus ID: 15976
• UniProt ID: O35664
• Cytogenetics: 16 52.82 cM
• Gene Summary: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 2 and isoform 3 may be potent inhibitors of type I IFN receptor activity.[UniProtKB/Swiss-Prot Function]