Trpm3 (NM_001035241) Mouse Tagged ORF Clone

CAT#: MG225391

Trpm3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 3 (Trpm3) transcript variant 3, (10ug)

Product Images

Circular map for MG225391

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm3
• Synonyms: 6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225391 representing NM_001035241
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA
  GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA
  GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA
  TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC
  TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT
  CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT
  TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA
  CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC
  CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC
  AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG
  GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG
  AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT
  ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA
  AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG
  AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG
  GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC
  TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT
  GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC
  ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA
  TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA
  CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA
  GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA
  TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT
  GTACCACTTGGTCAGGGATGTCAAAAAGCGAGAGTATCCAGGTTTCGGTTGGATCTATTTTAAGGGAAAC
  CTGCCCCCGGACTACAGAATCAGCCTGATAGACATTGGCCTGGTGATCGAGTACCTGATGGGCGGGGCTT
  ACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCCTCTACCACAATCTCTTTGGCCCCAAAAGGGATGA
  TATTCCCTTGAGGAGAGGACGGAAGACAACTAAGAAGCGTGAGGAAGAGGTGGACATCGACTTGGATGAT
  CCTGAGATCAACCACTTTCCATTCCCCTTCCATGAGCTGATGGTGTGGGCCGTCCTCATGAAGAGACAAA
  AGATGGCCCTGTTCTTCTGGCAACACGGAGAGGAGGCCATGGCCAAAGCCCTGGTGGCCTGCAAGCTCTG
  CAAGGCCATGGCTCACGAGGCTTCTGAGAATGACATGGTCGATGACATTTCCCAGGAGCTGAACCACAAC
  TCCAGGGACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAACAGGATGAGCAGCTGGCCA
  TGAAGCTGCTGACGTATGAGCTGAAGAACTGGAGTAATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAA
  GCACCGTGACTTCATTGCACACACGTGCAGCCAGATGTTACTTACTGACATGTGGATGGGACGGCTCCGG
  ATGAGAAAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGT
  TCAAGAACAAAGATGACATGCCCTATATGACTCAGGCCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGA
  GGAGCCAGAGAAGCCCACAAAGGAAAAAGATGAAGAGGACATGGAGCTAACAGCAATGTTGGGGCGAAGC
  AATGGGGAATCATCCAGAAAGAAAGATGAAGAAGAAGTTCAAAGCAGGCACCGGCTAATCCCCGTGGGCC
  GAAAAATCTATGAGTTCTACAACGCACCCATCGTGAAGTTCTGGTTCTACACTCTGGCGTATATTGGATA
  CCTGATGCTCTTCAACTACATCGTGTTAGTGAAGATGGAGCGCTGGCCTTCCACTCAGGAATGGATTGTC
  ATCTCCTACATTTTCACTCTGGGAATAGAGAAGATGAGAGAGATCCTGATGTCGGAGCCGGGCAAGCTGC
  TGCAGAAGGTGAAGGTGTGGCTTCAGGAGTACTGGAACGTCACAGACCTCATCGCCATCCTTCTCTTCTC
  GGTGGGAATGATCCTTCGTCTTCAAGATCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGTGTGAAC
  ATCATTTATTGGTATATCCGTTTGCTGGACATCTTCGGCGTGAACAAGTATCTGGGCCCATATGTGATGA
  TGATTGGGAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTGCTGATGAGCTTTGG
  GGTCGCCAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCATCTTGGAAACTGGCCAAGAATATCTTCTAC
  ATGCCTTATTGGATGATTTATGGGGAAGTGTTTGCTGACCAGATAGACCCTCCCTGTGGACAGAATGAGA
  CCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCATGCAAGACAGGAGCATGGATTGTCCCGGCCATCAT
  GGCCTGCTACCTCTTGGTGGCGAACATCCTTCTGGTCAACCTCCTTATTGCCGTCTTCAACAATACATTT
  TTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAATTTCAGAGGTACCAGCTCATCATGACTTTCCACG
  AGAGGCCGGTTCTGCCCCCACCGCTCATCATCTTCAGTCACATGACCATGATCTTCCAGCATGTGTGCTG
  CCGGTGGAGGAAGCATGAGAGTGACCAGGACGAAAGGGACTACGGCCTGAAACTCTTCATAACTGACGAT
  GAGCTCAAGAAAGTACACGATTTTGAAGAGCAGTGCATAGAGGAATATTTCCGAGAGAAGGATGATCGCT
  TCAATTCGTCCAACGATGAGAGGATACGTGTTACATCAGAAAGGGTGGAGAACATGTCCATGAGGCTGGA
  GGAAGTTAATGAGAGAGAACATTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTAGCACAGCTA
  GAGGACCTCATCGGGCGCATGGCCACCGCCCTGGAGCGCCTGACTGGTCTGGAGCGGGCAGAGTCTAACA
  AAATCCGCTCAAGGACCTCCTCAGACTGCACAGATGCAGCCTACATCGTCCGCCAGAGCAGCTTCAACAG
  CCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCATATCCCCAACT
  TCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCAGTCAATGTGAAAGACAAAGGTGGGA
  TAGAAAAGTTGGAAAGCATTTTCAAAGAAAGGTCCCTGAGCTTACACCGAGCTACTAGCTCCCACTCAGT
  AGCCAAAGAACCCAAAGCTCCTGCAGCCCCTGCAAACACCTTGGCCATTGTTCCTGACTCCAGAAGACCA
  TCATCTTGCATAGACATCTATGTCTCTGCCATGGACGAGCTCCACTGTGATATAGAGCCTCTGGATAATT
  CCATGAACATCCTTGGGCTGGGTGAGCCAAGCTTTTCAGCTCTAGCACCTTCCACAACCCCGTCAAGTAG
  TGCCTATGCAACGCTCGCACCTACAGACCGACCTCCCAGTAGGAGCATTGATTTTGAAGACCTCACCTCC
  ATGGACACTAGATCTTTTTCTTCAGACTATACACACCTCCCAGAATGCCAAAACCCCTGGGACACAGACC
  CTCCAACGTACCATACCATCGAGCGTTCCAAGAGTAGCCGCTACCTAGCCACCACGCCCTTTCTTCTGGA
  AGAGGCTCCCATTGTAAAATCCCATAGCTTTATGTTTTCTCCTTCAAGGAGCTACTACGCCAACTTTGGG
  GTGCCCGTGAAAACGGCAGAATACACAAGTATTACAGACTGTATCGACACAAGATGTGTCAATGCCCCCC
  AAGCAATAGCTGACCGAGCCACCTTCCCTGGAGGTCTCGGAGACAAAGTGGAAGATTTATCTTGTTGCCA
  CCCTGAGCGAGAAGCAGAGCTGAGCCATCCTAGCTCTGACAGTGAGGAAAATGAGGCCAGAGGCCAGAGA
  GCTGCCAATCCGATATCCTCTCAGGAGGCTGAAAATGCAGACAGAACCCTATCCAACAACATCACAGTTC
  CCAAGATAGAGCGCGCCAACAGCTACTCAGCAGAGGAGCCAAACGTGCCATATGCACATACCAGAAAGAG
  CTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAACACGGCGAGCCTCCGAAATCCCTTCCAGAGAAGT
  AAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTATCCATGAGGAGACTGTCTAGAACATCGGCCTTTC
  ATAGCTTTGAAAGCAAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225391 representing NM_001035241
  Red=Cloning site Green=Tags(s)
  
  MGKKWRDAGELERGCSDREDSAESRRRSRSASRGRFAESWKRLSSKQGSTKRSGLPAQQTPAQKSWIERA
  FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPSISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDA
  FGTIEFQGGGHSNKAMYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLI
  KAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNP
  MSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVL
  EYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQHLFIILMEC
  MKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQ
  AMLDALVLDRVDFVKLLIENGVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKREYPGFGWIYFKGN
  LPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRDDIPLRRGRKTTKKREEEVDIDLDD
  PEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHN
  SRDFGQLAVELLDQSYKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLR
  MRKNSGLKVILGILLPPSILSLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRS
  NGESSRKKDEEEVQSRHRLIPVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIV
  ISYIFTLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVN
  IIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFY
  MPYWMIYGEVFADQIDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTF
  FEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHMTMIFQHVCCRWRKHESDQDERDYGLKLFITDD
  ELKKVHDFEEQCIEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASLQTVDIRLAQL
  EDLIGRMATALERLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKLQESIDPAGEETISPT
  SPTLMPRMRSHSFYSVNVKDKGGIEKLESIFKERSLSLHRATSSHSVAKEPKAPAAPANTLAIVPDSRRP
  SSCIDIYVSAMDELHCDIEPLDNSMNILGLGEPSFSALAPSTTPSSSAYATLAPTDRPPSRSIDFEDLTS
  MDTRSFSSDYTHLPECQNPWDTDPPTYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFSPSRSYYANFG
  VPVKTAEYTSITDCIDTRCVNAPQAIADRATFPGGLGDKVEDLSCCHPEREAELSHPSSDSEENEARGQR
  AANPISSQEAENADRTLSNNITVPKIERANSYSAEEPNVPYAHTRKSFSISDKLDRQRNTASLRNPFQRS
  KSSKPEGRGDSLSMRRLSRTSAFHSFESKHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035241
• ORF Size: 5133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035241.3
• RefSeq Size: 6712 bp
• RefSeq ORF: 5136 bp
• Locus ID: 226025
• Cytogenetics: 19 B