Grn (NM_008175) Mouse Tagged ORF Clone

CAT#: MG225358

Grn (tGFP-tagged) - Mouse granulin (Grn), (10ug)

Product Images

Circular map for MG225358

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grn
• Synonyms: epithelin; Pgrn
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225358 representing NM_008175
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCCCAGGGAGCCCGGACCCCGACGCAGGCAGACCATGTGGGTCCTGATGAGCTGGCTGGCCTTCG
  CGGCAGGGCTGGTAGCCGGAACACAGTGTCCAGATGGGCAGTTCTGCCCTGTTGCCTGCTGCCTTGACCA
  GGGAGGAGCCAACTACAGCTGCTGTAACCCTCTTCTGGACACATGGCCTAGAATAACGAGCCATCATCTA
  GATGGCTCCTGCCAGACCCATGGCCACTGTCCTGCTGGCTATTCTTGTCTTCTCACTGTGTCTGGGACTT
  CCAGCTGCTGCCCGTTCTCTAAGGGTGTGTCTTGTGGTGATGGCTACCACTGCTGCCCCCAGGGCTTCCA
  CTGTAGTGCAGATGGGAAATCCTGCTTCCAGATGTCAGATAACCCCTTGGGTGCTGTCCAGTGTCCTGGG
  AGCCAGTTTGAATGTCCTGACTCTGCCACCTGCTGCATTATGGTTGATGGTTCGTGGGGATGTTGTCCCA
  TGCCCCAGGCCTCTTGCTGTGAAGACAGAGTGCATTGCTGTCCCCATGGGGCCTCCTGTGACCTGGTTCA
  CACACGATGCGTTTCACCCACGGGCACCCACACCCTACTAAAGAAGTTCCCTGCACAAAAGACCAACAGG
  GCAGTGTCTTTGCCTTTTTCTGTCGTGTGCCCTGATGCTAAGACCCAGTGTCCCGATGATTCTACCTGCT
  GTGAGCTACCCACTGGGAAGTATGGCTGCTGTCCAATGCCCAATGCCATCTGCTGTTCCGACCACCTGCA
  CTGCTGCCCCCAGGACACTGTATGTGACCTGATCCAGAGTAAGTGCCTATCCAAGAACTACACCACGGAT
  CTCCTGACCAAGCTGCCTGGATACCCAGTGAAGGAGGTGAAGTGCGACATGGAGGTGAGCTGCCCTGAAG
  GATATACCTGCTGCCGCCTCAACACTGGGGCCTGGGGCTGCTGTCCATTTGCCAAGGCCGTGTGTTGTGA
  GGATCACATTCATTGCTGCCCGGCAGGGTTTCAGTGTCACACAGAGAAAGGAACCTGCGAAATGGGTATC
  CTCCAAGTACCCTGGATGAAGAAGGTCATAGCCCCCCTCCGCCTGCCAGACCCACAGATCTTGAAGAGTG
  ATACACCTTGTGATGACTTCACTAGGTGTCCTACAAACAATACCTGCTGCAAACTCAATTCTGGGGACTG
  GGGCTGCTGTCCCATCCCAGAGGCTGTCTGCTGCTCAGACAACCAGCATTGCTGCCCTCAGGGCTTCACA
  TGTCTGGCTCAGGGGTACTGTCAGAAGGGAGACACAATGGTGGCTGGCCTGGAGAAGATACCTGCCCGCC
  AGACAACCCCGCTCCAAATTGGAGATATCGGTTGTGACCAGCATACCAGCTGCCCAGTAGGGCAAACCTG
  CTGCCCAAGCCTCAAGGGAAGTTGGGCCTGCTGCCAGCTGCCCCATGCTGTGTGCTGTGAGGACCGGCAG
  CACTGTTGCCCGGCCGGGTACACCTGCAATGTGAAGGCGAGGACCTGTGAGAAGGATGTCGATTTTATCC
  AGCCTCCCGTGCTCCTGACCCTCGGCCCTAAGGTTGGGAATGTGGAGTGTGGAGAAGGGCATTTCTGCCA
  TGATAACCAGACCTGTTGTAAAGACAGTGCAGGAGTCTGGGCCTGCTGTCCCTACCTAAAGGGTGTCTGC
  TGTAGAGATGGACGTCACTGTTGCCCCGGTGGCTTCCACTGTTCAGCCAGGGGAACCAAGTGTTTGCGAA
  AGAAGATTCCTCGCTGGGACATGTTTTTGAGGGATCCGGTCCCAAGACCGCTACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225358 representing NM_008175
  Red=Cloning site Green=Tags(s)
  
  MPPREPGPRRRQTMWVLMSWLAFAAGLVAGTQCPDGQFCPVACCLDQGGANYSCCNPLLDTWPRITSHHL
  DGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSDNPLGAVQCPG
  SQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNR
  AVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTD
  LLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGI
  LQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFT
  CLAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQ
  HCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVC
  CRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDPVPRPLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008175
• ORF Size: 1806 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008175.4, NP_032201.2
• RefSeq Size: 2348 bp
• RefSeq ORF: 1809 bp
• Locus ID: 14824
• UniProt ID: P28798
• Cytogenetics: 11 66.29 cM
• Gene Summary: Secreted proteins that act as key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed:28073925, PubMed:8496151, PubMed:28541286, PubMed:28453791, PubMed:20026663, PubMed:23041626, PubMed:27789271, PubMed:12524533). Functions as regulator of proteins trafficking to lysosome and activity of lysosomal enzymes (PubMed:28453791, PubMed:28541286, PubMed:27789271). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed:28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structure (PubMed:12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed:8496151). Moreover, modulates inflammation in neuron by preserving neuron survival, axonal outgrowth and neuronal integrity (PubMed:23041626, PubMed:20026663).[UniProtKB/Swiss-Prot Function]