Col1a2 (NM_007743) Mouse Tagged ORF Clone

CAT#: MG225252

Col1a2 (tGFP-tagged) - Mouse collagen type I alpha 2 (Col1a2), (10ug)

Product Images

Circular map for MG225252

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Col1a2
• Synonyms: AA960264; AI325291; Co; Col; Col1; Col1a-2; Cola-2; Cola2; oim
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225252 representing NM_007743
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCAGCTTTGTGGATACGCGGACTCTGTTGCTGCTTGCAGTAACTTCGTGCCTAGCAACATGCCAAT
  ATTTGCAATCGGGATCAGTACGAAAGGGCCCCACTGGAGACAGAGGACCACGTGGACAAAGGGGTCCCGC
  AGGTCCCCGAGGCAGAGATGGTGTTGATGGTCCCATGGGCCCTCCTGGTCCCCCTGGCTCCCCTGGTCCT
  CCCGGCTCCCCTGCCCCCCCTGGTCTTACTGGGAACTTTGCTGCTCAGTATTCTGACAAAGGAGTTTCAT
  CTGGCCCTGGACCAATGGGTTTAATGGGACCCAGAGGCCCTCCTGGTGCTGTTGGAGCCCCTGGCCCTCA
  AGGTTTCCAAGGACCTGCTGGTGAACCTGGTGAGCCTGGTCAAACGGGTCCTGCAGGTCCCCGTGGTCCA
  GCTGGCTCTCCTGGCAAGGCTGGTGAGGATGGTCACCCTGGAAAACCCGGAAGACCTGGGGAGAGAGGAG
  TCGTTGGACCACAGGGTGCTCGTGGTTTCCCCGGGACTCCTGGACTTCCTGGCTTCAAAGGCGTGAAAGG
  ACACAGTGGTATGGATGGATTAAAGGGACAGCCTGGTGCACAGGGTGTGAAGGGTGAACCCGGTGCCCCT
  GGAGAGAATGGAACTCCAGGTCAAGCAGGAGCCCGAGGGCTTCCTGGTGAGAGAGGGCGTGTCGGAGCTC
  CTGGTCCAGCTGGTGCCCGAGGCAGTGATGGAAGCGTTGGCCCCGTCGGCCCTGCTGGTCCCATTGGGTC
  TGCTGGACCCCCTGGTTTCCCAGGTGCTCCTGGTCCCAAGGGTGAACTTGGGCCCGTTGGTAACCCTGGC
  CCTGCTGGTCCTGCTGGTCCCCGTGGTGAAGTGGGTCTTCCAGGTCTTTCTGGCCCTGTTGGACCTCCTG
  GCAACCCTGGAACAAATGGGCTCACTGGCGCCAAGGGTGCTACTGGACTCCCTGGTGTCGCTGGGGCTCC
  CGGTCTCCCTGGCCCCCGTGGTATTCCTGGCCCCGCTGGTGCTGCTGGTGCTACAGGTGCCAGAGGACTT
  GTTGGTGAGCCTGGTCCTGCTGGCTCCAAAGGAGAATCCGGTAACAAGGGTGAGCCTGGCTCTGTTGGAG
  CCCAAGGTCCTCCTGGTCCCAGCGGTGAAGAAGGAAAGAGAGGGTCTCCCGGAGAAGCTGGATCTGCTGG
  CCCTGCAGGGCCTCCAGGGCTTAGAGGCAGCCCTGGTTCTCGAGGTCTTCCTGGAGCTGATGGCAGAGCT
  GGTGTAATGGGTCCTCCTGGCAATCGTGGTTCAACCGGCCCTGCTGGAATCCGAGGTCCTAATGGAGATG
  CCGGTCGACCTGGGGAACCTGGTCTCATGGGACCCAGAGGTCTTCCTGGGTCTCCTGGAAATGTTGGCCC
  ATCTGGTAAAGAAGGCCCTGTGGGTCTCCCTGGCATTGATGGCAGACCTGGCCCAATCGGCCCAGCTGGA
  CCAAGAGGTGAAGCTGGCAACATTGGATTCCCTGGACCCAAAGGCCCCTCTGGTGATCCTGGCAAACCTG
  GTGAGAGAGGACACCCCGGTCTTGCTGGTGCTCGGGGAGCTCCAGGACCCGATGGCAACAATGGAGCTCA
  GGGCCCCCCTGGACCCCAGGGTGTTCAAGGTGGCAAAGGTGAACAAGGCCCTGCTGGTCCTCCTGGCTTC
  CAGGGTCTCCCTGGTCCCTCAGGTACTACTGGAGAAGTTGGCAAGCCCGGAGAAAGGGGTCTTCCTGGTG
  AATTCGGTCTCCCTGGTCCTGCTGGTCCAAGAGGAGAACGTGGTACCCCGGGTGAGAGTGGAGCTGCTGG
  CCCTTCTGGTCCTATTGGAAGCCGAGGTCCCAGTGGAGCCCCAGGGCCTGATGGAAACAAGGGTGAAGCT
  GGTGCAGTCGGTGCTCCAGGCAGTGCTGGTGCCTCTGGGCCTGGTGGGCTTCCAGGAGAGAGGGGTGCTG
  CTGGCATACCTGGGGGCAAAGGAGAAAAGGGTGAAACTGGTCTCCGAGGTGACACTGGCAACACTGGTAG
  AGATGGTGCTCGTGGCATTCCTGGTGCTGTAGGTGCCCCTGGTCCTGCTGGGGCCTCAGGTGACCGGGGT
  GAAGCTGGTGCTGCCGGTCCTTCTGGCCCAGCTGGTCCTCGGGGTAGCCCTGGTGAACGTGGTGAAGTTG
  GCCCTGCTGGCCCCAATGGATTTGCTGGTCCTGCTGGTGCTGCTGGCCAACCCGGTGCTAAAGGAGAGAA
  GGGAACCAAAGGGCCTAAGGGTGAAAATGGCATTGTTGGTCCAACCGGGTCTGTTGGAGCTGCTGGCCCA
  TCTGGTCCTAATGGCCCACCTGGTCCTGTTGGAAGTCGTGGTGATGGAGGGCCCCCTGGTATGACTGGCT
  TCCCTGGTGCTGCTGGACGGACTGGTCCCCCCGGACCCAGTGGTATTGCTGGCCCCCCTGGCCCCCCTGG
  TGCTGCTGGAAAGGAAGGGATTCGTGGTCCCCGTGGCGACCAAGGTCCAGTTGGCCGAACTGGAGAAACA
  GGTGCATCTGGACCCCCTGGATTTGTTGGTGAAAAGGGTCCCTCTGGAGAACCTGGTACCGCAGGAGCCC
  CTGGTACCGCAGGTCCTCAGGGTCTTCTTGGTGCTCCCGGTATTCTGGGTCTCCCTGGCTCTAGAGGTGA
  ACGTGGTCTGCCAGGTATTGCTGGTGCTCTGGGTGAACCTGGTCCTCTGGGCATCTCAGGCCCTCCTGGT
  GCCCGTGGTCCCCCTGGTGCTGTGGGCAGCCCTGGAGTTAATGGTGCCCCTGGTGAAGCTGGTCGTGATG
  GTAACCCTGGCAGCGATGGTCCCCCAGGTCGTGATGGTCAGCCTGGACACAAGGGAGAGCGTGGTTACCC
  TGGCAGCATTGGTCCGACTGGTGCTGCAGGTGCCCCTGGTCCTCACGGTTCTGTGGGTCCTGCTGGGAAA
  CATGGAAACCGAGGTGAACCTGGTCCTGCCGGTTCTGTTGGTCCTGTTGGTGCTGTTGGTCCAAGAGGTC
  CTAGTGGTCCTCAAGGCATCCGAGGTGACAAAGGAGAACCTGGTGATAAAGGGCACAGGGGTCTTCCTGG
  CTTAAAAGGATACAGTGGATTGCAGGGTCTTCCTGGTCTTGCTGGCCTACATGGTGACCAAGGAGCTCCT
  GGTCCTGTGGGTCCTGCTGGTCCCAGGGGTCCTGCTGGGCCTTCTGGACCCGTTGGCAAAGATGGTAGAT
  CTGGGCAACCTGGCCCAGTCGGGCCTGCTGGTGTTCGTGGTTCTCAGGGTAGCCAAGGTCCCGCTGGTCC
  CCCTGGTCCCCCTGGACCTCCTGGTCCTCCTGGTGTCAGCGGAGGTGGCTATGACTTTGGTTTTGAAGGA
  GACTTCTACAGGGCTGACCAGCCTCGCTCCCAGCCTTCACTCAGACCCAAGGACTATGAAGTTGATGCAA
  CTCTGAAATCTCTCAATAACCAGATTGAGACCCTTCTCACTCCTGAAGGCTCTAGAAAGAACCCTGCTCG
  CACGTGCCGGGACTTAAGACTCAGCCACCCAGAGTGGAACAGCGATTACTACTGGATTGACCCTAACCAA
  GGATGTACTATGGATGCCATCAAAGTGTACTGTGATTTCTCTACTGGTGAAACCTGCATCCAGGCCCAAC
  CTGTAAACACCCCAGCGAAGAACTCATACAGCCGCGCCCAGGCCAACAAGCATGTCTGGTTAGGAGAGAC
  TATCAATGGTGGCAGCCAGTTTGAATACAACGTAGAAGGGGTGTCCTCCAAGGAAATGGCAACTCAGCTC
  GCCTTCATGCGCCTGCTAGCCAACCGTGCTTCTCAGAACATCACCTACCACTGCAAGAACAGCATTGCGT
  ACCTGGATGAGGAGACGGGCAGCTTGAACAAGGCTGTGCTTCTGCAGGGTTCCAACGATGTTGAACTTGT
  TGCTGAGGGCAACAGCAGGTTCACCTACTCTGTCCTAGTCGATGGCTGCTCCAAAAAGACAAATGAATGG
  GGCAAGACAATCATTGAATACAAAACAAATAAGCCATCTCGCCTGCCGTTCCTTGACATTGCACCTCTGG
  ACATTGGTGGTGCTGACCAAGAATTCCGTGTGGAGGTTGGCCCCGTCTGTTTCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225252 representing NM_007743
  Red=Cloning site Green=Tags(s)
  
  MLSFVDTRTLLLLAVTSCLATCQYLQSGSVRKGPTGDRGPRGQRGPAGPRGRDGVDGPMGPPGPPGSPGP
  PGSPAPPGLTGNFAAQYSDKGVSSGPGPMGLMGPRGPPGAVGAPGPQGFQGPAGEPGEPGQTGPAGPRGP
  AGSPGKAGEDGHPGKPGRPGERGVVGPQGARGFPGTPGLPGFKGVKGHSGMDGLKGQPGAQGVKGEPGAP
  GENGTPGQAGARGLPGERGRVGAPGPAGARGSDGSVGPVGPAGPIGSAGPPGFPGAPGPKGELGPVGNPG
  PAGPAGPRGEVGLPGLSGPVGPPGNPGTNGLTGAKGATGLPGVAGAPGLPGPRGIPGPAGAAGATGARGL
  VGEPGPAGSKGESGNKGEPGSVGAQGPPGPSGEEGKRGSPGEAGSAGPAGPPGLRGSPGSRGLPGADGRA
  GVMGPPGNRGSTGPAGIRGPNGDAGRPGEPGLMGPRGLPGSPGNVGPSGKEGPVGLPGIDGRPGPIGPAG
  PRGEAGNIGFPGPKGPSGDPGKPGERGHPGLAGARGAPGPDGNNGAQGPPGPQGVQGGKGEQGPAGPPGF
  QGLPGPSGTTGEVGKPGERGLPGEFGLPGPAGPRGERGTPGESGAAGPSGPIGSRGPSGAPGPDGNKGEA
  GAVGAPGSAGASGPGGLPGERGAAGIPGGKGEKGETGLRGDTGNTGRDGARGIPGAVGAPGPAGASGDRG
  EAGAAGPSGPAGPRGSPGERGEVGPAGPNGFAGPAGAAGQPGAKGEKGTKGPKGENGIVGPTGSVGAAGP
  SGPNGPPGPVGSRGDGGPPGMTGFPGAAGRTGPPGPSGIAGPPGPPGAAGKEGIRGPRGDQGPVGRTGET
  GASGPPGFVGEKGPSGEPGTAGAPGTAGPQGLLGAPGILGLPGSRGERGLPGIAGALGEPGPLGISGPPG
  ARGPPGAVGSPGVNGAPGEAGRDGNPGSDGPPGRDGQPGHKGERGYPGSIGPTGAAGAPGPHGSVGPAGK
  HGNRGEPGPAGSVGPVGAVGPRGPSGPQGIRGDKGEPGDKGHRGLPGLKGYSGLQGLPGLAGLHGDQGAP
  GPVGPAGPRGPAGPSGPVGKDGRSGQPGPVGPAGVRGSQGSQGPAGPPGPPGPPGPPGVSGGGYDFGFEG
  DFYRADQPRSQPSLRPKDYEVDATLKSLNNQIETLLTPEGSRKNPARTCRDLRLSHPEWNSDYYWIDPNQ
  GCTMDAIKVYCDFSTGETCIQAQPVNTPAKNSYSRAQANKHVWLGETINGGSQFEYNVEGVSSKEMATQL
  AFMRLLANRASQNITYHCKNSIAYLDEETGSLNKAVLLQGSNDVELVAEGNSRFTYSVLVDGCSKKTNEW
  GKTIIEYKTNKPSRLPFLDIAPLDIGGADQEFRVEVGPVCFK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007743
• ORF Size: 4116 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007743.2
• RefSeq Size: 5143 bp
• RefSeq ORF: 4119 bp
• Locus ID: 12843
• UniProt ID: Q01149
• Cytogenetics: 6 1.81 cM
• Gene Summary: This gene encodes the alpha-2 subunit of the fibril-forming type I collagen, the most abundant protein of bone, skin and tendon extracellular matrices. The encoded protein, in association with alpha-1 subunit, forms heterotrimeric type I procollagen that undergoes proteolytic processing during fibril formation. Mice harboring certain mutations in the encoded gene exhibit symptoms of moderate to severe forms of osteogenesis imperfecta. [provided by RefSeq, Dec 2015]