Trpm3 (NM_001035242) Mouse Tagged ORF Clone

CAT#: MG225062

Trpm3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 3 (Trpm3) transcript variant 4, (10ug)

Product Images

Circular map for MG225062

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm3
• Synonyms: 6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG225062 representing NM_001035242
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA
  GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA
  GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA
  TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC
  TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT
  CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT
  TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA
  CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC
  CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC
  AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG
  GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG
  AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT
  ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA
  AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG
  AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG
  GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC
  TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT
  GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC
  ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA
  TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA
  CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA
  GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA
  TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT
  GTACCACTTGGTCAGGGATGTCAAAAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATT
  GGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCC
  TCTACCACAATCTCTTTGGCCCCAAAAGGCCCAAAGCCTTGAAACTGCTGGGAATGGAGGATGATATTCC
  CTTGAGGAGAGGACGGAAGACAACTAAGAAGCGTGAGGAAGAGGTGGACATCGACTTGGATGATCCTGAG
  ATCAACCACTTTCCATTCCCCTTCCATGAGCTGATGGTGTGGGCCGTCCTCATGAAGAGACAAAAGATGG
  CCCTGTTCTTCTGGCAACACGGAGAGGAGGCCATGGCCAAAGCCCTGGTGGCCTGCAAGCTCTGCAAGGC
  CATGGCTCACGAGGCTTCTGAGAATGACATGGTCGATGACATTTCCCAGGAGCTGAACCACAACTCCAGG
  GACTTTGGCCAGCTGGCTGTGGAGCTCCTGGACCAGTCCTACAAACAGGATGAGCAGCTGGCCATGAAGC
  TGCTGACGTATGAGCTGAAGAACTGGAGTAATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCG
  TGACTTCATTGCACACACGTGCAGCCAGATGTTACTTACTGACATGTGGATGGGACGGCTCCGGATGAGA
  AAGAACTCAGGCCTCAAGGTAATTCTGGGAATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGA
  ACAAAGATGACATGCCCTATATGACTCAGGCCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGAGGAGCC
  AGAGAAGCCCACAAAGGAAAAAGATGAAGAGGACATGGAGCTAACAGCAATGTTGGGGCGAAGCAATGGG
  GAATCATCCAGAAAGAAAGATGAAGAAGAAGTTCAAAGCAGGCACCGGCTAATCCCCGTGGGCCGAAAAA
  TCTATGAGTTCTACAACGCACCCATCGTGAAGTTCTGGTTCTACACTCTGGCGTATATTGGATACCTGAT
  GCTCTTCAACTACATCGTGTTAGTGAAGATGGAGCGCTGGCCTTCCACTCAGGAATGGATTGTCATCTCC
  TACATTTTCACTCTGGGAATAGAGAAGATGAGAGAGATCCTGATGTCGGAGCCGGGCAAGCTGCTGCAGA
  AGGTGAAGGTGTGGCTTCAGGAGTACTGGAACGTCACAGACCTCATCGCCATCCTTCTCTTCTCGGTGGG
  AATGATCCTTCGTCTTCAAGATCAGCCCTTCAGGAGTGACGGGAGGGTCATCTACTGTGTGAACATCATT
  TATTGGTATATCCGTTTGCTGGACATCTTCGGCGTGAACAAGTATCTGGGCCCATATGTGATGATGATTG
  GGAAAATGATGATAGACATGATGTACTTTGTCATCATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGC
  CAGGCAAGCCATTCTCTTTCCCAATGAGGAGCCATCTTGGAAACTGGCCAAGAATATCTTCTACATGCCT
  TATTGGATGATTTATGGGGAAGTGTTTGCTGACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAG
  AGGATGGCAAGACAATCCAGCTGCCCCCATGCAAGACAGGAGCATGGATTGTCCCGGCCATCATGGCCTG
  CTACCTCTTGGTGGCGAACATCCTTCTGGTCAACCTCCTTATTGCCGTCTTCAACAATACATTTTTTGAG
  GTCAAGTCGATATCCAACCAAGTATGGAAATTTCAGAGGTACCAGCTCATCATGACTTTCCACGAGAGGC
  CGGTTCTGCCCCCACCGCTCATCATCTTCAGTCACATGACCATGATCTTCCAGCATGTGTGCTGCCGGTG
  GAGGAAGCATGAGAGTGACCAGGACGAAAGGGACTACGGCCTGAAACTCTTCATAACTGACGATGAGCTC
  AAGAAAGTACACGATTTTGAAGAGCAGTGCATAGAGGAATATTTCCGAGAGAAGGATGATCGCTTCAATT
  CGTCCAACGATGAGAGGATACGTGTTACATCAGAAAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGT
  TAATGAGAGAGAACATTCCATGAAGGCTTCACTCCAGACCGTGGACATCCGGCTAGCACAGCTAGAGGAC
  CTCATCGGGCGCATGGCCACCGCCCTGGAGCGCCTGACTGGTCTGGAGCGGGCAGAGTCTAACAAAATCC
  GCTCAAGGACCTCCTCAGACTGCACAGATGCAGCCTACATCGTCCGCCAGAGCAGCTTCAACAGCCAGGA
  AGGGAACACCTTCAAACTTCAAGAGAGTATAGACCCTGCAGGTGAGGAGACCATATCCCCAACTTCTCCA
  ACCTTAATGCCCCGTATGCGAAGCCATTCTTTCTATTCAGTCAATGTGAAAGACAAAGGTGGGATAGAAA
  AGTTGGAAAGCATTTTCAAAGAAAGGTCCCTGAGCTTACACCGAGCTACTAGCTCCCACTCAGTAGCCAA
  AGAACCCAAAGCTCCTGCAGCCCCTGCAAACACCTTGGCCATTGTTCCTGACTCCAGAAGACCATCATCT
  TGCATAGACATCTATGTCTCTGCCATGGACGAGCTCCACTGTGATATAGAGCCTCTGGATAATTCCATGA
  ACATCCTTGGGCTGGGTGAGCCAAGCTTTTCAGCTCTAGCACCTTCCACAACCCCGTCAAGTAGTGCCTA
  TGCAACGCTCGCACCTACAGACCGACCTCCCAGTAGGAGCATTGATTTTGAAGACCTCACCTCCATGGAC
  ACTAGATCTTTTTCTTCAGACTATACACACCTCCCAGAATGCCAAAACCCCTGGGACACAGACCCTCCAA
  CGTACCATACCATCGAGCGTTCCAAGAGTAGCCGCTACCTAGCCACCACGCCCTTTCTTCTGGAAGAGGC
  TCCCATTGTAAAATCCCATAGCTTTATGTTTTCTCCTTCAAGGAGCTACTACGCCAACTTTGGGGTGCCC
  GTGAAAACGGCAGAATACACAAGTATTACAGACTGTATCGACACAAGATGTGTCAATGCCCCCCAAGCAA
  TAGCTGACCGAGCCACCTTCCCTGGAGGTCTCGGAGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGA
  GCGAGAAGCAGAGCTGAGCCATCCTAGCTCTGACAGTGAGGAAAATGAGGCCAGAGGCCAGAGAGCTGCC
  AATCCGATATCCTCTCAGGAGGCTGAAAATGCAGACAGAACCCTATCCAACAACATCACAGTTCCCAAGA
  TAGAGCGCGCCAACAGCTACTCAGCAGAGGAGCCAAACGTGCCATATGCACATACCAGAAAGAGCTTCTC
  CATCAGTGACAAGCTTGATAGACAGAGGAACACGGCGAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCC
  TCCAAGCCGGAGGGCCGAGGGGACAGCCTATCCATGAGGAGACTGTCTAGAACATCGGCCTTTCATAGCT
  TTGAAAGCAAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG225062 representing NM_001035242
  Red=Cloning site Green=Tags(s)
  
  MGKKWRDAGELERGCSDREDSAESRRRSRSASRGRFAESWKRLSSKQGSTKRSGLPAQQTPAQKSWIERA
  FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPSISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDA
  FGTIEFQGGGHSNKAMYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLI
  KAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNP
  MSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVL
  EYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQHLFIILMEC
  MKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQ
  AMLDALVLDRVDFVKLLIENGVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDI
  GLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDIPLRRGRKTTKKREEEVDIDLDDPE
  INHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHNSR
  DFGQLAVELLDQSYKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMR
  KNSGLKVILGILLPPSILSLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRSNG
  ESSRKKDEEEVQSRHRLIPVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIVIS
  YIFTLGIEKMREILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVNII
  YWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFYMP
  YWMIYGEVFADQIDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFE
  VKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHMTMIFQHVCCRWRKHESDQDERDYGLKLFITDDEL
  KKVHDFEEQCIEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASLQTVDIRLAQLED
  LIGRMATALERLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKLQESIDPAGEETISPTSP
  TLMPRMRSHSFYSVNVKDKGGIEKLESIFKERSLSLHRATSSHSVAKEPKAPAAPANTLAIVPDSRRPSS
  CIDIYVSAMDELHCDIEPLDNSMNILGLGEPSFSALAPSTTPSSSAYATLAPTDRPPSRSIDFEDLTSMD
  TRSFSSDYTHLPECQNPWDTDPPTYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFSPSRSYYANFGVP
  VKTAEYTSITDCIDTRCVNAPQAIADRATFPGGLGDKVEDLSCCHPEREAELSHPSSDSEENEARGQRAA
  NPISSQEAENADRTLSNNITVPKIERANSYSAEEPNVPYAHTRKSFSISDKLDRQRNTASLRNPFQRSKS
  SKPEGRGDSLSMRRLSRTSAFHSFESKHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035242
• ORF Size: 5127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035242.2
• RefSeq Size: 6706 bp
• RefSeq ORF: 5130 bp
• Locus ID: 226025
• Cytogenetics: 19 B