Trpm3 (NM_001035243) Mouse Tagged ORF Clone

CAT#: MG224633

Trpm3 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 3 (Trpm3) transcript variant 5, (10ug)

Product Images

Circular map for MG224633

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trpm3
• Synonyms: 6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224633 representing NM_001035243
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA
  GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA
  GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA
  TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC
  TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT
  CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT
  TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA
  CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC
  CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC
  AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG
  GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG
  AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT
  ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA
  AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG
  AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG
  GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC
  TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT
  GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC
  ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA
  TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA
  CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA
  GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA
  TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT
  GTACCACTTGGTCAGGGATGTCAAAAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATT
  GGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCC
  TCTACCACAATCTCTTTGGCCCCAAAAGGGATGATATTCCCTTGAGGAGAGGACGGAAGACAACTAAGAA
  GCGTGAGGAAGAGGTGGACATCGACTTGGATGATCCTGAGATCAACCACTTTCCATTCCCCTTCCATGAG
  CTGATGGTGTGGGCCGTCCTCATGAAGAGACAAAAGATGGCCCTGTTCTTCTGGCAACACGGAGAGGAGG
  CCATGGCCAAAGCCCTGGTGGCCTGCAAGCTCTGCAAGGCCATGGCTCACGAGGCTTCTGAGAATGACAT
  GGTCGATGACATTTCCCAGGAGCTGAACCACAACTCCAGGGACTTTGGCCAGCTGGCTGTGGAGCTCCTG
  GACCAGTCCTACAAACAGGATGAGCAGCTGGCCATGAAGCTGCTGACGTATGAGCTGAAGAACTGGAGTA
  ATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCGTGACTTCATTGCACACACGTGCAGCCAGAT
  GTTACTTACTGACATGTGGATGGGACGGCTCCGGATGAGAAAGAACTCAGGCCTCAAGGTAATTCTGGGA
  ATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGATGACATGCCCTATATGACTCAGG
  CCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGAGGAGCCAGAGAAGCCCACAAAGGAAAAAGATGAAGA
  GGACATGGAGCTAACAGCAATGTTGGGGCGAAGCAATGGGGAATCATCCAGAAAGAAAGATGAAGAAGAA
  GTTCAAAGCAGGCACCGGCTAATCCCCGTGGGCCGAAAAATCTATGAGTTCTACAACGCACCCATCGTGA
  AGTTCTGGTTCTACACTCTGGCGTATATTGGATACCTGATGCTCTTCAACTACATCGTGTTAGTGAAGAT
  GGAGCGCTGGCCTTCCACTCAGGAATGGATTGTCATCTCCTACATTTTCACTCTGGGAATAGAGAAGATG
  AGAGAGATCCTGATGTCGGAGCCGGGCAAGCTGCTGCAGAAGGTGAAGGTGTGGCTTCAGGAGTACTGGA
  ACGTCACAGACCTCATCGCCATCCTTCTCTTCTCGGTGGGAATGATCCTTCGTCTTCAAGATCAGCCCTT
  CAGGAGTGACGGGAGGGTCATCTACTGTGTGAACATCATTTATTGGTATATCCGTTTGCTGGACATCTTC
  GGCGTGAACAAGTATCTGGGCCCATATGTGATGATGATTGGGAAAATGATGATAGACATGATGTACTTTG
  TCATCATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATTCTCTTTCCCAATGAGGA
  GCCATCTTGGAAACTGGCCAAGAATATCTTCTACATGCCTTATTGGATGATTTATGGGGAAGTGTTTGCT
  GACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCAT
  GCAAGACAGGAGCATGGATTGTCCCGGCCATCATGGCCTGCTACCTCTTGGTGGCGAACATCCTTCTGGT
  CAACCTCCTTATTGCCGTCTTCAACAATACATTTTTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAA
  TTTCAGAGGTACCAGCTCATCATGACTTTCCACGAGAGGCCGGTTCTGCCCCCACCGCTCATCATCTTCA
  GTCACATGACCATGATCTTCCAGCATGTGTGCTGCCGGTGGAGGAAGCATGAGAGTGACCAGGACGAAAG
  GGACTACGGCCTGAAACTCTTCATAACTGACGATGAGCTCAAGAAAGTACACGATTTTGAAGAGCAGTGC
  ATAGAGGAATATTTCCGAGAGAAGGATGATCGCTTCAATTCGTCCAACGATGAGAGGATACGTGTTACAT
  CAGAAAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGTTAATGAGAGAGAACATTCCATGAAGGCTTC
  ACTCCAGACCGTGGACATCCGGCTAGCACAGCTAGAGGACCTCATCGGGCGCATGGCCACCGCCCTGGAG
  CGCCTGACTGGTCTGGAGCGGGCAGAGTCTAACAAAATCCGCTCAAGGACCTCCTCAGACTGCACAGATG
  CAGCCTACATCGTCCGCCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTAT
  AGACCCTGCAGGTGAGGAGACCATATCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCT
  TTCTATTCAGTCAATGTGAAAGACAAAGGTGGGATAGAAAAGTTGGAAAGCATTTTCAAAGAAAGGTCCC
  TGAGCTTACACCGAGCTACTAGCTCCCACTCAGTAGCCAAAGAACCCAAAGCTCCTGCAGCCCCTGCAAA
  CACCTTGGCCATTGTTCCTGACTCCAGAAGACCATCATCTTGCATAGACATCTATGTCTCTGCCATGGAC
  GAGCTCCACTGTGATATAGAGCCTCTGGATAATTCCATGAACATCCTTGGGCTGGGTGAGCCAAGCTTTT
  CAGCTCTAGCACCTTCCACAACCCCGTCAAGTAGTGCCTATGCAACGCTCGCACCTACAGACCGACCTCC
  CAGTAGGAGCATTGATTTTGAAGACCTCACCTCCATGGACACTAGATCTTTTTCTTCAGACTATACACAC
  CTCCCAGAATGCCAAAACCCCTGGGACACAGACCCTCCAACGTACCATACCATCGAGCGTTCCAAGAGTA
  GCCGCTACCTAGCCACCACGCCCTTTCTTCTGGAAGAGGCTCCCATTGTAAAATCCCATAGCTTTATGTT
  TTCTCCTTCAAGGAGCTACTACGCCAACTTTGGGGTGCCCGTGAAAACGGCAGAATACACAAGTATTACA
  GACTGTATCGACACAAGATGTGTCAATGCCCCCCAAGCAATAGCTGACCGAGCCACCTTCCCTGGAGGTC
  TCGGAGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGAGCGAGAAGCAGAGCTGAGCCATCCTAGCTC
  TGACAGTGAGGAAAATGAGGCCAGAGGCCAGAGAGCTGCCAATCCGATATCCTCTCAGGAGGCTGAAAAT
  GCAGACAGAACCCTATCCAACAACATCACAGTTCCCAAGATAGAGCGCGCCAACAGCTACTCAGCAGAGG
  AGCCAAACGTGCCATATGCACATACCAGAAAGAGCTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAA
  CACGGCGAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTA
  TCCATGAGGAGACTGTCTAGAACATCGGCCTTTCATAGCTTTGAAAGCAAGCACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224633 representing NM_001035243
  Red=Cloning site Green=Tags(s)
  
  MGKKWRDAGELERGCSDREDSAESRRRSRSASRGRFAESWKRLSSKQGSTKRSGLPAQQTPAQKSWIERA
  FYKRECVHIIPSTKDPHRCCCGRLIGQHVGLTPSISVLQNEKNESRLSRNDIQSEKWSISKHTQLSPTDA
  FGTIEFQGGGHSNKAMYVRVSFDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLI
  KAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLIGRDVVRPYQTMSNP
  MSKLTVLNSMHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVL
  EYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGLINESLRDQLLVTIQKTFTYTRTQAQHLFIILMEC
  MKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQ
  AMLDALVLDRVDFVKLLIENGVSMHRFLTISRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDI
  GLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRDDIPLRRGRKTTKKREEEVDIDLDDPEINHFPFPFHE
  LMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNHNSRDFGQLAVELL
  DQSYKQDEQLAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILG
  ILLPPSILSLEFKNKDDMPYMTQAQEIHLQEKEPEEPEKPTKEKDEEDMELTAMLGRSNGESSRKKDEEE
  VQSRHRLIPVGRKIYEFYNAPIVKFWFYTLAYIGYLMLFNYIVLVKMERWPSTQEWIVISYIFTLGIEKM
  REILMSEPGKLLQKVKVWLQEYWNVTDLIAILLFSVGMILRLQDQPFRSDGRVIYCVNIIYWYIRLLDIF
  GVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILFPNEEPSWKLAKNIFYMPYWMIYGEVFA
  DQIDPPCGQNETREDGKTIQLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWK
  FQRYQLIMTFHERPVLPPPLIIFSHMTMIFQHVCCRWRKHESDQDERDYGLKLFITDDELKKVHDFEEQC
  IEEYFREKDDRFNSSNDERIRVTSERVENMSMRLEEVNEREHSMKASLQTVDIRLAQLEDLIGRMATALE
  RLTGLERAESNKIRSRTSSDCTDAAYIVRQSSFNSQEGNTFKLQESIDPAGEETISPTSPTLMPRMRSHS
  FYSVNVKDKGGIEKLESIFKERSLSLHRATSSHSVAKEPKAPAAPANTLAIVPDSRRPSSCIDIYVSAMD
  ELHCDIEPLDNSMNILGLGEPSFSALAPSTTPSSSAYATLAPTDRPPSRSIDFEDLTSMDTRSFSSDYTH
  LPECQNPWDTDPPTYHTIERSKSSRYLATTPFLLEEAPIVKSHSFMFSPSRSYYANFGVPVKTAEYTSIT
  DCIDTRCVNAPQAIADRATFPGGLGDKVEDLSCCHPEREAELSHPSSDSEENEARGQRAANPISSQEAEN
  ADRTLSNNITVPKIERANSYSAEEPNVPYAHTRKSFSISDKLDRQRNTASLRNPFQRSKSSKPEGRGDSL
  SMRRLSRTSAFHSFESKHN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001035243
• ORF Size: 5097 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001035243.3
• RefSeq Size: 6676 bp
• RefSeq ORF: 5100 bp
• Locus ID: 226025
• Cytogenetics: 19 B