Ebf2 (NM_010095) Mouse Tagged ORF Clone

CAT#: MG224591

Ebf2 (tGFP-tagged) - Mouse early B-cell factor 2 (Ebf2), (10ug)

Product Images

Circular map for MG224591

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ebf2
• Synonyms: D14Ggc1e; EBF-2; Mmot1; O/E-3; OE-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224591 representing NM_010095
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGGATTCAAGATACGCTAGGAAGAGGACCAGCTCTGAAAGACAAGTCGCTGGGTGCCGAGATGG
  ATTCGGTCAGGTCCTGGGTCCGGAATGTCGGCGTGGTGGACGCCAATGTCGCGGCTCAGAGCGGAGTTGC
  TCTGTCCCGGGCCCATTTTGAAAAGCAACCTCCTTCAAACTTGAGGAAATCCAACTTCTTTCATTTTGTC
  CTGGCGCTCTACGACAGGCAGGGACAGCCGGTGGAGATTGAGCGCACAGCCTTCGTGGACTTTGTGGAGA
  ATGACAAAGAGCAAGGCAATGAGAAGACCAACAATGGTACTCACTACAAGTTACAGCTCCTTTACAGCAA
  CGGTGTCCGCACGGAGCAGGACCTGTATGTCAGGCTCATCGACTCTGTCACCAAACAGCCCATAGCCTAT
  GAGGGACAGAATAAGAATCCCGAAATGTGCAGAGTTCTGCTGACACACGAGGTGATGTGCAGTCGATGCT
  GTGAGAAGAAGAGCTGCGGGAACCGGAACGAGACCCCTTCAGACCCGGTCATCATCGACAGATTCTTTTT
  AAAATTTTTCCTGAAGTGCAATCAGAATTGTTTGAAAACAGCGGGAAACCCAAGGGACATGAGGCGGTTC
  CAGGTCGTGTTGTCCACAACAGTAAATGTGGATGGACATGTGCTGGCTGTTTCTGACAACATGTTTGTTC
  ATAACAATTCTAAGCATGGAAGGAGAGCAAGAAGGCTTGACCCATCTGAAGCTACACCCTGCATCAAAGC
  CATCAGCCCAAGTGAAGGCTGGACCACAGGAGGGGCCATGGTCATCATCATTGGTGACAACTTTTTTGAT
  GGTCTCCAGGTTGTGTTTGGTACCATGCTTGTATGGAGTGAGCTCATAACCCCTCATGCCATCAGAGTAC
  AGACCCCTCCCCGGCATATCCCTGGAGTTGTGGAAGTGACACTATCTTATAAATCCAAACAGTTCTGCAA
  AGGAGCCCCGGGCAGGTTCATCTACACAGCCTTAAATGAACCCACCATAGACTATGGCTTCCAAAGATTG
  CAGAAAGTCATCCCAAGGCACCCAGGTGACCCTGAGAGGCTAGCTAAGGAAATGCTGTTGAAAAGAGCTG
  CGGACCTGGTGGAAGCCCTCTACGGCACACCACACAATAATCAGGACATCATTTTAAAGCGTGCTGCAGA
  CATTGCTGAAGCCCTGTACAGTGTCCCCAGGAACCCCAGCCAGATTCCAGCTCTTTCCAGCTCCCCAGCT
  CACAGCGGTATGATGGGTATCAACTCTTACGGCAGCCAGCTCGGCGTCAGCATTTCAGAGTCCACACAAG
  GAAATAACCAAGGATACATCCGCAACACAAGCAGCATCTCCCCGCGGGGATACTCCTCCAGCTCCACGCC
  TCAGCAATCTAATTACAGCACCTCCAGCAACAGCATGAACGGCTACAGCAATGTTCCTATGGCCAACCTG
  GGTGTGCCAGGATCCCCGGGATTTCTGAATGGCTCACCCACGGGCTCCCCATATGGAATCATGTCTTCAA
  GCCCCACCGTGGGATCCTCCAGCACGTCCTCCATCCTCCCGTTCTCCTCTTCAGTTTTTCCTGCTGTCAA
  ACAGAAGAGTGCCTTTGCCCCTGTCATCAGGCCCCAAGGCTCCCCATCGCCTGCCTGCTCCAGCGGCAAT
  GGGAACGGATTCAGAGCCATGACAGGACTTGTTGTTCCCCCGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224591 representing NM_010095
  Red=Cloning site Green=Tags(s)
  
  MFGIQDTLGRGPALKDKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFV
  LALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAY
  EGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRF
  QVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFD
  GLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRL
  QKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQIPALSSSPA
  HSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANL
  GVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGN
  GNGFRAMTGLVVPPM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010095
• ORF Size: 1725 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010095.6, NP_034225.1
• RefSeq Size: 5527 bp
• RefSeq ORF: 1728 bp
• Locus ID: 13592
• UniProt ID: O08792
• Cytogenetics: 14 D1
• Gene Summary: Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.[UniProtKB/Swiss-Prot Function]