Pgd (NM_001081274) Mouse Tagged ORF Clone

CAT#: MG224503

Pgd (tGFP-tagged) - Mouse phosphogluconate dehydrogenase (Pgd), (10ug)

Product Images

Circular map for MG224503

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pgd
• Synonyms: 0610042A05Rik; AU019875; C78335
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224503 representing NM_001081274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAAGCTGACATTGCACTGATCGGACTGGCTGTCATGGGCCAGAACTTAATTTTGAACATGAATG
  ATCATGGATTTGTGGTCTGTGCTTTCAATAGGACAGTCTCCAAAGTCGATGACTTCTTGGCCAACGAGGC
  GAAGGGCACCAAGGTGGTTGGTGCACAGTCCTTAAAGGACATGGTCTCCAAACTAAAGAAGCCCCGCCGG
  GTCATCCTGCTTGTGAAGGCCGGGCAAGCCGTGGATGATTTCATCGAGAAATTAGTACCCTTGTTGGACA
  CGGGTGACATCATCATCGATGGAGGAAATTCTGAATACCGGGACACGACAAGAAGATGCCGGGACCTCAA
  GGCCAAGGGCATCTTGTTTGTGGGGAGCGGAGTCAGTGGTGGTGAGGAAGGGGCTCGGTACGGGCCGTCA
  CTCATGCCAGGAGGGAACAAAGAGGCTTGGCCCCACATCAAGGCGATCTTCCAAGCCATCGCTGCAAAAG
  TGGGAACCGGAGAACCCTGCTGTGACTGGGTGGGAGATGAGGGGGCGGGGCACTTTGTGAAGATGGTGCA
  CAACGGGATAGAGTATGGAGACATGCAGCTCATCTGTGAGGCTTACCATTTGATGAAGGATGTTCTGGGC
  ATGCGGCATGAGGAGATGGCTCAGGCATTTGAAGAATGGAACAAGACAGAGCTGGACTCATTCCTGATTG
  AAATCACTGCTAACATTCTCAAGTACCGGGACACTGACGGCAAAGAGCTGTTGCCAAAGATCCGGGACAG
  TGCTGGGCAGAAGGGCACTGGGAAGTGGACCGCCATCTCGGCGCTGGAGTACGGCATGCCCGTCACCCTC
  ATTGGAGAAGCTGTCTTTGCTCGGTGCTTGTCCTCTCTGAAGGAGGAGCGGGTTCAGGCCAGCCAAAAGC
  TGAAGGGTCCTAAGGTGGTCCAGCTGGAAGGCAGTAAGAAGTCATTCCTGGAGGACATCCGTAAGGCCCT
  CTATGCTTCCAAGATCATCTCCTACGCCCAAGGCTTTATGCTGCTCAGACAGGCAGCCACTGAGTTTGGC
  TGGACCCTCAATTATGGCGCCATTGCCCTGATGTGGAGAGGGGGCTGCATCATCCGAAGTGTGTTCCTGG
  GAAAAATTAAAGATGCATTTGAGCGAAACCCAGAACTTCAGAACCTACTGCTAGATGACTTCTTTAAGTC
  AGCAGTTGACAACTGCCAGGACTCCTGGCGGCGGGTGATCAGCACTGGGGTGCAAGCAGGCATTCCCATG
  CCCTGCTTCACTACTGCCCTCTCCTTCTATGATGGGTACAGACACGAGATGCTGCCAGCAAACCTCATCC
  AGGCTCAACGGGATTACTTTGGGGCTCACACCTATGAACTCTTAACCAAACCGGGAGAATTTATCCACAC
  CAACTGGACGGGCCACGGGGGCAGTGTGTCATCCTCTTCATACAATGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224503 representing NM_001081274
  Red=Cloning site Green=Tags(s)
  
  MAQADIALIGLAVMGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAQSLKDMVSKLKKPRR
  VILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARYGPS
  LMPGGNKEAWPHIKAIFQAIAAKVGTGEPCCDWVGDEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLG
  MRHEEMAQAFEEWNKTELDSFLIEITANILKYRDTDGKELLPKIRDSAGQKGTGKWTAISALEYGMPVTL
  IGEAVFARCLSSLKEERVQASQKLKGPKVVQLEGSKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFG
  WTLNYGAIALMWRGGCIIRSVFLGKIKDAFERNPELQNLLLDDFFKSAVDNCQDSWRRVISTGVQAGIPM
  PCFTTALSFYDGYRHEMLPANLIQAQRDYFGAHTYELLTKPGEFIHTNWTGHGGSVSSSSYNA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081274
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081274.1, NP_001074743.1
• RefSeq Size: 2224 bp
• RefSeq ORF: 1452 bp
• Locus ID: 110208
• UniProt ID: Q9DCD0
• Cytogenetics: 4 78.96 cM
• Gene Summary: Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.[UniProtKB/Swiss-Prot Function]