Itga7 (NM_008398) Mouse Tagged ORF Clone

CAT#: MG224446

Itga7 (tGFP-tagged) - Mouse integrin alpha 7 (Itga7), (10ug)

Product Images

Circular map for MG224446

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itga7
• Synonyms: al; alpha7; [a]; [a]7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG224446 representing NM_008398
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGATTCCGAGGTGCGATTTTCTCCGGCCCCCCGGAATCTACTATCTTATTACTTCCTTGCTCG
  CTGGACTGTTCTTACCACCGGCTATCGCCTTCAATCTGGATGTGATGGGTGCCATACGCAAGGAGGGAGA
  ACCCGGCAGCCTATTCGGCTTTTCGGTGGCCCTGCACCGACAATTACAGCCCCGACCCCAGAGCTGGCTG
  CTGGTGGGCGCTCCCCAGGCCCTGGCTCTTCCTGGACAGCAGGCAAACCGCACCGGAGGCCTCTTTGCTT
  GTCCCCTGAGCCTAGAGGAGACAGATTGCTACAGAGTGGACATTGACAGGGGAGCTAACGTGCAGAAGGA
  GAGCAAGGAGAACCAATGGCTGGGAGTCAGTGTCCGGAGCCAGGGGCCAGGGGGCAAGATTGTTACGTGT
  GCACACCGATATGAGTCTCGACAGAGAGTGGACCAGGCTTTGGAGACTCGGGATGTGATTGGCCGCTGCT
  TTGTGCTAAGCCAGGACCTGGCCATCCGTGATGAGCTGGATGGTGGGGAGTGGAAGTTCTGTGAGGGGCG
  CCCCCAGGGCCATGAACAGTTTGGGTTCTGTCAGCAGGGCACAGCTGCCACCTTCTCCCCTGACAGTCAC
  TACCTCGTCTTTGGGGCTCCAGGAACCTATAACTGGAAGGGGTTGCTTTTTGTGACCAACATTGATAGCT
  CAGACCCTGACCAGCTGGTGTATAAAACTTTGGACCCTGCTGACCGGCTCACAGGACCAGCCGGAGACTT
  GACCTTGAATAGCTATTTAGGTTTCTCCATCGATTCTGGGAAGGGTCTTATGCGCTCAGAAGAGCTGAGT
  TTTGTGGCAGGGGCCCCACGTGCCAACCACAAAGGGGCTGTGGTCATTCTGCGCAAGGATAGCGCCAGCC
  GCTTGATACCTGAGGTTGTGCTGTCTGGGGAGCGCCTGACCTCTGGCTTTGGCTACTCACTGGCTGTGAC
  TGATCTCAACAATGATGGCTGGGCAGACCTGATTGTGGGTGCCCCCTACTTCTTTGAACGCCAAGAAGAG
  CTGGGAGGTGCTGTGTATGTGTACATGAACCAGGGTGGCCATTGGGCAGATATCTCTCCTCTCCGAATCT
  GTGGCTCCCCTGATTCCATGTTTGGGATCAGTTTGGCTGTATTGGGGGACCTCAACCAAGATGGCTTCCC
  AGACATTGCCGTGGGAGCTCCCTTTGACGGAGATGGGAAGGTCTTTATCTACCATGGGAGCAGCCTTGGG
  GTGGTTGTCAAACCTTCACAGGTGCTGGAGGGCGAGGCGGTGGGTATCAAGAGCTTTGGTTACTCCCTGT
  CTGGTGGCCTGGATGTGGATGGAAATCACTACCCAGACCTGCTTGTGGGCTCCCTGGCTGATACCGCTGC
  TCTGTTCAGGGCCAGACCTGTTCTACATGTCTCCCAAGAGATCTTCATTGACCCCAGAGCCATCGATCTG
  GAACAACCCAACTGTGCCGATGGACGCTTGGTCTGTGTGGACATAAAGATCTGTTTCAGCTATGTCGCTG
  TGCCCAGCAGCTACAGCCCTAGTGTGGCCTTAGATTATATGTTAGATGGGGACACAGACCGGAGGCTCCG
  GGGCCAGGTTCCACGTGTGACGTTTCTGAGCCGAGGCCTGGATGACCTCAGGCATCAGTCCTCCGGCACC
  GTGTGGCTGAAGCACCAACATGACCGAGTCTGTGGAGATACTGTGTTCCAGCTGCAGGAAAACGTCAAAG
  ACAAGCTACGGGCCATTGTGGTGACCCTGTCATATGGTCTCCGAACCCCTCCATTAGGGAGGCAAGCACC
  TGGCCAGGAGCTCCCCACTGTGGCTCCCATCCTCAATGCTCACCAGCCCAGCACCCAGAGGACTGAGATC
  CACTTTCTGAAGCAAGGCTGCGGTGAAGATAAGATCTGTCAGAGCAACCTCCAGCTCGAGCGGTACCAGT
  TCTGTTCCAGGATCAGCGACACAGAGTTCCAGGCTCTGCCCATGGATCTGGATGGAAGGACCGCCCTGTT
  CGCATTGAGTGGGCAGCCGTTCATAGGCCTGGAACTGACAGTCACCAACCTGCCCTCTGACCCTGCCCGG
  CCTCAGGCAGATGGGGATGATGCTCATGAAGCACAGCTCTTGGTCACTCTCCCAGCCTCTCTACGGTACT
  CAGGAGTCCGTGCTCTGGACTCTGTGGAGAAGCCGCTCTGCCTGTCCAATGACAGCGCCTCTCATGTCGA
  GTGTGAGCTGGGGAACCCTATGAAGAGAGGCGCTCAGGTCACTTTTTACCTCATCCTCAGCACCTCTGGG
  ATCACTATTGAGACCACAGAGCTGGAGGTGAAATTGCTGTTAGCCACGATCAGTGAGCAGGAGCTGGATC
  CGGTCTCCGTTCGGGCTCATGTCTTCATTGAATTGCCACTGTCCATTTCAGGGGTGGCCACTCCCCAGCA
  ACTCTTCTTCTCTGGCGAGGTGAAGGGCGAAAGTGCCATGCGATCTGAGAGGGACGTGGGCAGCAAAGTC
  AAGTATGAGGTCACGGTCTCCAATCAAGGCCAGTCTCTCAATACTCTGGGCTCTGCGTTCCTCAACATCA
  TGTGGCCCCACGAGATCGCCAATGGAAAGTGGCTGCTGTACCCCATGCGGGTAGAGCTGGAGGGCGGACA
  GGGGCCTGGCAAGAGAGGGATCTGTTCCCCAAGACCCAACATCCTCCAGCTGGATGTGGACAGCAGGGAT
  AGGAGGCGGCGAGAGCTGGGGCAGCCGGAGCCGCAGGAGCCTCCAGAGAAGGTGGAGCCTAGCACATCCT
  GGTGGCCAGTGTCCTCTGCTGAGAAGAGAAACGTGACTCTGGACTGCGCCCAGGGCACGGCCAAGTGTGT
  GGTGTTCAGCTGCCCACTCTACAGCTTTGATCGTGCAGCCGTGCTTCACGTCTGGGGCCGCCTCTGGAAC
  AGCACCTTTCTGGAGGAGTACATGGCCGTGAAATCCCTGGAAGTGATCGTCCGAGCCAACATCACAGTGA
  AATCCTCCATTAAGAACTTGTTGCTCAGAGATGCATCCACAGTGATTCCAGTGATGGTGTACTTGGACCC
  CATGGCCGTGGTTGTGGAAGGAGTCCCCTGGTGGGTCATCCTCCTGGCAGTGCTGGCTGGGCTGTTAGTC
  CTGGCCTTGCTGGTACTGCTGCTGTGGAAGCTGGGATTCTTCAAGCGGGCGAAGCACCCCGAGGCCACCG
  TGCCCCAGTACCACGCAGTGAAGATCCCTCGGGAAGACCGACAGCAGTTCAAGGAGGAGAAGACAGGCAC
  CATCCAGAGGAGTAACTGGGGCAACTCCCAGTGGGAGGGCTCCGACGCACACCCCATCTTGGCTGCCGAC
  TGGCATCCTGAGCTGGGTCCTGATGGACATCCGGTGCCAGCCACTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG224446 representing NM_008398
  Red=Cloning site Green=Tags(s)
  
  MARIPRCDFLRPPGIYYLITSLLAGLFLPPAIAFNLDVMGAIRKEGEPGSLFGFSVALHRQLQPRPQSWL
  LVGAPQALALPGQQANRTGGLFACPLSLEETDCYRVDIDRGANVQKESKENQWLGVSVRSQGPGGKIVTC
  AHRYESRQRVDQALETRDVIGRCFVLSQDLAIRDELDGGEWKFCEGRPQGHEQFGFCQQGTAATFSPDSH
  YLVFGAPGTYNWKGLLFVTNIDSSDPDQLVYKTLDPADRLTGPAGDLTLNSYLGFSIDSGKGLMRSEELS
  FVAGAPRANHKGAVVILRKDSASRLIPEVVLSGERLTSGFGYSLAVTDLNNDGWADLIVGAPYFFERQEE
  LGGAVYVYMNQGGHWADISPLRICGSPDSMFGISLAVLGDLNQDGFPDIAVGAPFDGDGKVFIYHGSSLG
  VVVKPSQVLEGEAVGIKSFGYSLSGGLDVDGNHYPDLLVGSLADTAALFRARPVLHVSQEIFIDPRAIDL
  EQPNCADGRLVCVDIKICFSYVAVPSSYSPSVALDYMLDGDTDRRLRGQVPRVTFLSRGLDDLRHQSSGT
  VWLKHQHDRVCGDTVFQLQENVKDKLRAIVVTLSYGLRTPPLGRQAPGQELPTVAPILNAHQPSTQRTEI
  HFLKQGCGEDKICQSNLQLERYQFCSRISDTEFQALPMDLDGRTALFALSGQPFIGLELTVTNLPSDPAR
  PQADGDDAHEAQLLVTLPASLRYSGVRALDSVEKPLCLSNDSASHVECELGNPMKRGAQVTFYLILSTSG
  ITIETTELEVKLLLATISEQELDPVSVRAHVFIELPLSISGVATPQQLFFSGEVKGESAMRSERDVGSKV
  KYEVTVSNQGQSLNTLGSAFLNIMWPHEIANGKWLLYPMRVELEGGQGPGKRGICSPRPNILQLDVDSRD
  RRRRELGQPEPQEPPEKVEPSTSWWPVSSAEKRNVTLDCAQGTAKCVVFSCPLYSFDRAAVLHVWGRLWN
  STFLEEYMAVKSLEVIVRANITVKSSIKNLLLRDASTVIPVMVYLDPMAVVVEGVPWWVILLAVLAGLLV
  LALLVLLLWKLGFFKRAKHPEATVPQYHAVKIPREDRQQFKEEKTGTIQRSNWGNSQWEGSDAHPILAAD
  WHPELGPDGHPVPATA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008398
• ORF Size: 3408 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008398.2, NP_032424.2
• RefSeq Size: 4035 bp
• RefSeq ORF: 3411 bp
• Locus ID: 16404
• Cytogenetics: 10 77.2 cM
• Gene Summary: This gene encodes a member of the integrin family of cell surface proteins that mediate cellular interactions with the extracellular matrix and other cells. The encoded transmembrane protein is the alpha subunit that forms a noncovalent heterodimer with the beta subunit to form the functional integrin receptor that binds to laminin. Mice lacking the encoded protein exhibit symptoms of progressive muscular dystrophy, impaired axonal regeneration and cerebral vascular defects. [provided by RefSeq, Aug 2016]