Ube2j2 (NM_001039158) Mouse Tagged ORF Clone
CAT#: MG224115
- TrueORF®
Ube2j2 (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2 J2 homolog (yeast) (Ube2j2) transcript variant 3, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001039158" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Ube2j2 |
Synonyms | 1200007B18Rik; 2400008G19Rik; 5730472G04Rik; AL022923; NCUBE-2; Ubc6; Ubc6p |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224115 representing NM_001039158
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAATAACAGCAATAAGAGAGCTCCAACAACAGCAACACAAAGGTTGAAGCAGGACTACCTTCGAA TTAAGAAAGACCCAGTGCCTTACATCTGTGCAGAGCCCCTCCCTTCAAATATTCTTGAATGGCATTATGT TGTCCGAGGCCCTGAGATGACTCCTTATGAAGGTGGCTATTATCATGGAAAACTAATTTTTCCCAGAGAA TTTCCATTTAAGCCTCCTAGTATTTACATGATAACCCCCAATGGAAGATTTAAGTGCAACACAAGGCTGT GTCTCTCCATCACAGATTTCCACCCAGATACATGGAATCCAGCGTGGTCCGTCTCCACCATTCTAACAGG GCTCCTGAGCTTTATGGTGGAGAAAGGCCCCACCCTGGGGAGCATAGAGACGTCGGACTTCACGAAAAAA CAGCTGGCAGCACAGAGTCTAGTGTTTAATTTAAAAGATAAAGTCTTTTGTGAATTATTTCCTGAAGTTG TGGAGGAAATTAAACAGAAACAGAAAGCACAAGATGAACTAAGTAACAGGCCCCAGAATCTTCCTTTGCC AGACGTGGTTCCTGATGGGGAGCTGCACCGTGGCCAGCATGGGATCCAGCTCCTCAATGGGCACGCACCC GCTGCTGGCCCCAACCTCGCTGGGCTCCCACAGGCCAACAGGCATCACGGACTCCTGGGCGGCGCGTTGG CGAACTTGTTTGTTATAGTTGGGTTTGCAGCCTTCGCCTACACGGTCAAGTACGTGCTGAGAAGCATAGC ACAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224115 representing NM_001039158
Red=Cloning site Green=Tags(s) MSNNSNKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGYYHGKLIFPRE FPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLSFMVEKGPTLGSIETSDFTKK QLAAQSLVFNLKDKVFCELFPEVVEEIKQKQKAQDELSNRPQNLPLPDVVPDGELHRGQHGIQLLNGHAP AAGPNLAGLPQANRHHGLLGGALANLFVIVGFAAFAYTVKYVLRSIAQE TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001039158 |
ORF Size | 777 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001039158.2 |
RefSeq Size | 3339 bp |
RefSeq ORF | 780 bp |
Locus ID | 140499 |
UniProt ID | Q6P073 |
Cytogenetics | 4 87.66 cM |
Gene Summary | Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR224115 | Ube2j2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2, J2 homolog (yeast) (Ube2j2), transcript variant 3 |
USD 330.00 |
|
MR224115L3 | Lenti ORF clone of Ube2j2 (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2, J2 homolog (yeast) (Ube2j2), transcript variant 3 |
USD 630.00 |
|
MR224115L4 | Lenti ORF clone of Ube2j2 (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2, J2 homolog (yeast) (Ube2j2), transcript variant 3 |
USD 630.00 |
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