Kcnt1 (NM_175462) Mouse Tagged ORF Clone

CAT#: MG223804

Kcnt1 (tGFP-tagged) - Mouse potassium channel subfamily T member 1 (Kcnt1) transcript variant 1, (10ug)

Product Images

Circular map for MG223804

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kcnt1
• Synonyms: C030030G16Rik; s; Sl; Slack; slo2; Slo2.2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223804 representing NM_175462
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGGCCAAGCTGCCGCGCTCGCCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCG
  CTGCAGCCCCTGAGGAGCCACACGGGCTCAGCCCGCTACTGCCGGCCCGCGGCGGGGGCTCCGTGGGCAG
  CGACGTGGGCCAGAGGCTTCATGTGGAAGATTTCAGCCTGGACTCTTCCCTTTCTCAGGTCCAGGTGGAG
  TTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTGTTCTTCATCAAAAACCAAAGATCCAGCC
  TGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCTGCCTGCTGTATATCGTCCGTGTCCTGCT
  TGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAAGTATAACTACACTTTCAATGGCTCATCC
  TCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAAATGGCTCTGTGGGTGATCCAGGTCATTG
  TGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACCTCAGCTACAAAGGCAACATCTGGGAGCA
  GATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACTGCCCTTCATCATCACGGTCTTCTGGCCA
  CCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGGCTGGCCAAGCATGCGCTGGAGAACATGA
  TTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCATGTTCAACCAGGTGCTCATCCTGTTCTG
  CACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCAGCACCTGGAGCGAGCAGGTGGCAACTTG
  AACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCAACTGTGGGCTTCGGTGATGTGACGCCCA
  AGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTGTCACCCTTGTGGTGCTCCCACTGCAATT
  TGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGGGGGCAACTATAGCCGCCACCGAGCACGG
  ACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATTGATCTCCTCATGGACTTCCTGAATGAGT
  TCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCCTGTGTCCCTCTGAAATGGACGTCCAGGT
  GCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCATCTACCTCCAGGGCTCTGCCCTCAAGGAC
  CAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGCTTTATCCTCAGCAGCAGGAATGAGGTGG
  ACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGGCTGTGAAGGACTTTGCCCCCAACTGTCC
  CCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGTCAAATTTGCTGACCACGTGGTATGCGAG
  GAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGCCCGGCCACCTCCACCCTCATCACCCTGC
  TGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCGAGCAGTGGCAGCGCACGTACGGGAGGTG
  CTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAAATTCTTCCGGGAGTATGAGGGCAAGAGC
  TTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTGTGCCTCATCGGGCTGAAGCGTGAGGAGA
  ACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGGCTGCCTCCGACACCTGCTTCTATATCAA
  TATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGAGGAGAAGCAGAAGAGACGGGGCCTTGCA
  GGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCATAGCATCATCGCCTCTATGGGGACAGTGG
  CCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTGGCAGTGGCGGGGACGGCACAAAGCTGAC
  TCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCATCGCACCCGTTCTGGAGTTGGCAGACAGC
  TCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAGGATGAGGTGACACCCTCGGACGACGAGG
  GGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACTCACCCTACATTGGCAGCTCCCCGACTTT
  ATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCGGTTGGACAAGGGCTGCAAACACAACAGC
  TACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATTATTGTCTCTGCTGAGACGGCAGGCAACG
  GGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGTCCCGCCGGGAGCTCAACCCTATCGTGTT
  GCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCATCTGTTGCTTCCCCATGGTCTACTACATG
  GAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGCATCATCTATGCTGACAACCTGGTGGTGG
  TGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGGCAGATGCCAAGACCATCGTCAATGTGCA
  AACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGAGCTCACACACCCTTCCAACATGCGGTTC
  ATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCCAAACTTGAAAAGCAAGAACGGGAGAACG
  GCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTGGTCGAGTATTTAGTATCAGCATGTTGGA
  TACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCACCATCACCAGGCTGCTCTTGGGCCTTGAT
  ACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACCGAGGACGACCTGTGGATCCGCACTTACG
  GCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCCCCATCGGCATCTACAGGACCGAGTGCCA
  TGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGATCTCGGTGAACATGGAGGACTGCGAGGAT
  ACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCTGGCAGTGGCAGCACCCATGGCCGTCACG
  GGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCAAGAGCCTGCAGTGGGCCCGCAAGCTGAG
  TCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCACAGACTGGATCACCCAGCAGCGGCTCAGC
  CTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTCAAGAACCGAATGAAGCACCTGGGACTGC
  CCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTGATGTGATGAATCGGGTAAACCTGGGATA
  TTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTCCTATGTACTCATCAACCCCCCGCCAGAC
  ACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCCGACCCCCTGGCCCATGTGGCCAGCAGCT
  CCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCTGTAATCCTGAGACCAGGGATGAGACCCA
  GCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223804 representing NM_175462
  Red=Cloning site Green=Tags(s)
  
  MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEDFSLDSSLSQVQVE
  FYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSS
  SEFHWAPILWVERKMALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWP
  PLRNLFIPVFLNCWLAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNL
  NLLTSFYFCIVTFSTVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRAR
  TEKHVVLCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKD
  QDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCE
  EECKYAMLALNCICPATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKS
  FTYAAFHAHKKYGVCLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLA
  GQALYEGPSRLPVHSIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADS
  SALLPCDLLSDQSEDEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNS
  YEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYM
  EGSVDNLDSLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRF
  MQFRAKDSYSLALSKLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLD
  TTPGSGYLCAMKVTEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCED
  TREAKGPWGTRAASGSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLS
  LYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPD
  TRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175462
• ORF Size: 3714 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175462.4, NP_780671.2
• RefSeq Size: 5893 bp
• RefSeq ORF: 3717 bp
• Locus ID: 227632
• UniProt ID: Q6ZPR4
• Cytogenetics: 2 A3
• Gene Summary: This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]