Il11ra1 (NM_010549) Mouse Tagged ORF Clone

CAT#: MG223675

Il11ra1 (tGFP-tagged) - Mouse interleukin 11 receptor alpha chain 1 (Il11ra1) transcript variant 1, (10ug)

Product Images

Circular map for MG223675

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Il11ra1
• Synonyms: AI314697; GP130; Il-11ra; Il11ra; Il11ra2; NR1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223675 representing NM_010549
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCAGCTGCTCAGGGCTGACCAGGGTCCTGGTGGCCGTGGCTACAGCCCTGGTGTCTTCCTCCT
  CCCCCTGCCCCCAAGCTTGGGGTCCTCCAGGGGTCCAGTATGGACAACCTGGCAGGCCCGTGATGCTGTG
  CTGCCCCGGAGTGAGTGCTGGGACTCCAGTGTCCTGGTTTCGGGATGGAGATTCAAGGCTGCTCCAGGGA
  CCTGACTCTGGGTTAGGACACAGACTGGTCTTAGCCCAGGTGGACAGCCCTGATGAAGGCACTTATATCT
  GCCAGACCCTGGATGGTGTATCAGGGGGCATGGTGACCCTGAAGCTGGGCTTTCCCCCAGCACGTCCTGA
  GGTCTCCTGCCAAGCGGTAGACTATGAAAACTTCTCCTGTACTTGGAGTCCAGGCCAGGTCAGCGGTTTG
  CCCACCCGCTACCTTACTTCCTACAGGAAGAAGACGCTGCCAGGAGCTGAGAGTCAGAGGGAAAGTCCAT
  CCACTGGGCCTTGGCCGTGTCCACAGGACCCTCTGGAGGCCTCCCGATGTGTGGTCCATGGGGCAGAGTT
  CTGGAGTGAGTACCGGATCAATGTGACCGAGGTGAACCCACTGGGTGCCAGCACGTGCCTACTGGATGTG
  AGATTACAGAGCATCTTGCGTCCCGATCCACCCCAAGGACTGCGGGTGGAATCGGTACCTGGTTACCCGA
  GACGCCTGCATGCCAGCTGGACATACCCTGCCTCCTGGCGTCGCCAACCCCACTTTCTGCTCAAGTTCCG
  GTTGCAATACCGACCAGCACAGCATCCAGCCTGGTCCACGGTGGAGCCCATTGGCTTGGAGGAAGTGATA
  ACAGATGCTGTGGCTGGGCTGCCACACGCGGTACGAGTCAGTGCCAGGGACTTTCTGGATGCTGGCACCT
  GGAGCGCCTGGAGCCCAGAGGCCTGGGGTACTCCTAGCACTGGTCCCCTGCAGGATGAGATACCTGATTG
  GAGCCAGGGACACAGACAGCAGCTAGAGGCAGTAGTAGCTCAGGAGGACAGCCCGGCTCCTGCAAGGCCT
  TCCTTGCAGCCGGACCCAAGGCCACTTGATCACAGGGACCCCTTGGAGCAAATAGCTGTGTTAGCGTCTC
  TGGGAATCTTCTCTTGCCTTGGCCTGGCTGTTGGAGCCCTGGCACTGGGGCTCTGGCTGAGGCTGAGACG
  GAGTGGGAAGGATGGACCGCAAAAACCTGGGCTCTTGGCACCCATGATCCCGGTGGAAAAGCTTCCAGGA
  ATTCCAAACCTGCAGAGGACCCCAGAGAACTTCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223675 representing NM_010549
  Red=Cloning site Green=Tags(s)
  
  MSSSCSGLTRVLVAVATALVSSSSPCPQAWGPPGVQYGQPGRPVMLCCPGVSAGTPVSWFRDGDSRLLQG
  PDSGLGHRLVLAQVDSPDEGTYICQTLDGVSGGMVTLKLGFPPARPEVSCQAVDYENFSCTWSPGQVSGL
  PTRYLTSYRKKTLPGAESQRESPSTGPWPCPQDPLEASRCVVHGAEFWSEYRINVTEVNPLGASTCLLDV
  RLQSILRPDPPQGLRVESVPGYPRRLHASWTYPASWRRQPHFLLKFRLQYRPAQHPAWSTVEPIGLEEVI
  TDAVAGLPHAVRVSARDFLDAGTWSAWSPEAWGTPSTGPLQDEIPDWSQGHRQQLEAVVAQEDSPAPARP
  SLQPDPRPLDHRDPLEQIAVLASLGIFSCLGLAVGALALGLWLRLRRSGKDGPQKPGLLAPMIPVEKLPG
  IPNLQRTPENFS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010549
• ORF Size: 1296 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010549.3, NP_034679.1
• RefSeq Size: 1766 bp
• RefSeq ORF: 1299 bp
• Locus ID: 16157
• UniProt ID: Q64385
• Cytogenetics: 4 21.94 cM
• Gene Summary: Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells. Essential for the normal development of craniofacial bones and teeth.[UniProtKB/Swiss-Prot Function]