Atp2b2 (NM_001036684) Mouse Tagged ORF Clone

CAT#: MG223661

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  • TrueORF®

Atp2b2 (GFP-tagged) - Mouse ATPase Ca++ transporting plasma membrane 2 (Atp2b2) transcript variant 2, (10ug)



  "NM_001036684" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,573.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Symbol Atp2b2
Synonyms D6Abb2e; dfw; Gena300; jog; PMCA2; Tmy; wms; wri
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG223661 representing NM_001036684
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT
TCGGGTGCACCATGGAGGAGCTGCGCTCCCTCATGGAGCTGCGGGGCACCGAGGCTGTGGTCAAGATCAA
GGAGACGTATGGGGACACTGAAGCCATCTGCCGGCGCCTCAAAACCTCGCCTGTTGAAGGTTTACCAGGC
ACTGCTCCAGACTTGGAAAAGAGGAAACAGATTTTTGGGCAAAACTTCATACCTCCAAAGAAACCAAAAA
CCTTCCTGCAGCTGGTGTGGGAAGCGCTACAGGACGTGACACTTATCATCCTGGAGATCGCGGCCATCAT
CTCCCTGGGACTGTCCTTCTACCACCCACCGGGAGAGAGCAATGAAGGATGTGCCACGGCCCAGGGTGGG
GCAGAGGATGAAGGTGAAGCAGAAGCAGGCTGGATTGAGGGGGCTGCCATCCTGCTGTCAGTCATCTGTG
TGGTCCTGGTCACAGCCTTCAATGACTGGAGTAAGGAGAAACAGTTCCGGGGCCTGCAGAGCCGAATTGA
GCAGGAGCAGAAGTTCACTGTGGTCCGGGCCGGCCAGGTGGTTCAGATCCCTGTGGCCGAGATCGTGGTC
GGGGACATTGCCCAGATCAAATATGGTGACCTTCTTCCCGCTGATGGCCTCTTCATCCAGGGAAATGACC
TCAAAATTGATGAAAGCTCACTCACAGGGGAATCTGACCAGGTGCGCAAGTCTGTGGATAAGGACCCCAT
GTTGCTGTCAGGAACCCATGTGATGGAGGGCTCAGGACGGATGGTGGTTACTGCTGTGGGTGTGAACTCT
CAGACTGGCATCATATTTACCCTGCTTGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGCCA
AGCAGCAGGATGGGGCAGCTGCCATGGAGATGCAGCCTCTGAAGAGTGCAGAGGGCGGCGATGCAGATGA
CAAGAAGAAAGCCAACATGCACAAGAAAGAGAAGTCGGTGCTTCAGGGCAAGCTCACCAAACTGGCTGTG
CAGATAGGCAAGGCGGGCCTGGTGATGTCGGCCATCACAGTGATCATCCTGGTACTCTACTTCACCGTGG
ACACCTTCGTGGTCAACAAGAAGCCATGGCTGACGGAATGCACACCCGTCTACGTACAGTACTTTGTCAA
GTTCTTCATCATTGGTGTGACGGTGCTGGTGGTCGCTGTGCCCGAGGGCCTCCCTCTGGCTGTCACCATC
TCACTGGCCTATTCTGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGA
CCATGGGCAATGCCACAGCCATCTGCTCAGACAAGACAGGAACGCTGACCACCAACCGCATGACCGTGGT
CCAGGCCTATGTCGGTGACGTCCACTACAAGGAGATCCCCGATCCCAGCTCCATCAATGCCAAGACGCTG
GAGCTGCTGGTCAACGCCATTGCCATCAACAGCGCCTACACCACCAAGATCCTTCCCCCAGAAAAAGAGG
GAGCCCTGCCCCGGCAGGTGGGCAACAAGACAGAGTGCGGCCTGCTGGGCTTTGTGCTGGACTTGAGGCA
GGACTACGAGCCGGTGCGCAGCCAGATGCCAGAGGAGAAGCTGTATAAGGTGTACACCTTCAACTCCGTG
CGCAAGTCCATGAGCACCGTCATCAAGATGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGCGCCTCGG
AGATTGTGCTCAAAAAGTGCTGCAAGATCCTCAGTGGGGCAGGGGAAGCCCGTGTCTTCCGGCCCCGAGA
CAGGGATGAGATGGTTAAGAAGGTGATTGAGCCCATGGCCTGTGACGGGCTCCGTACCATCTGCGTGGCC
TATCGTGACTTCCCCAGCAGCCCTGAGCCTGACTGGGACAATGAGAATGACATTCTCAATGAACTCACGT
GCATCTGCGTGGTGGGCATCGAAGACCCAGTACGACCTGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCG
GGCAGGTATCACAGTCCGCATGGTCACCGGTGACAATATCAACACAGCCCGGGCCATCGCCATCAAGTGT
GGCATTATCCACCCTGGAGAGGACTTCCTGTGCCTGGAAGGCAAAGAATTCAATCGGAGGATTCGCAACG
AGAAGGGGGAGATTGAGCAGGAGCGGATTGACAAGATCTGGCCAAAGCTGAGGGTGCTGGCTCGCTCCTC
GCCCACGGATAAGCACACGCTGGTCAAAGGCATCATCGACAGTACACACACTGAGCAGCGGCAGGTGGTG
GCTGTGACAGGGGATGGGACCAACGACGGGCCTGCTCTCAAGAAGGCAGATGTGGGCTTCGCAATGGGCA
TCGCAGGCACAGATGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGATGACAACTTCAGCAGCATCGT
CAAGGCAGTGATGTGGGGCCGTAACGTCTATGACAGCATATCCAAATTCCTGCAGTTCCAGCTGACTGTC
AACGTGGTGGCGGTGATCGTGGCCTTCACGGGCGCCTGCATTACACAGGACTCCCCTCTCAAGGCTGTGC
AGATGCTCTGGGTGAACCTCATCATGGACACGTTTGCCTCCCTGGCCCTGGCCACAGAGCCACCTACGGA
GACTCTGCTTCTGAGGAAACCGTACGGTCGCAACAAGCCGCTCATCTCGAGGACCATGATGAAGAACATC
CTGGGCCACGCCGTCTACCAGCTCACCCTCATCTTCACCCTGCTCTTCGTGGGTGAGAAGATGTTCCAGA
TCGACAGCGGAAGGAACGCCCCGCTGCACTCACCACCCTCAGAGCACTACACCATCATCTTCAACACCTT
CGTCATGATGCAGCTTTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAACGTCTTTGACGGG
ATCTTCCGGAACCCCATCTTCTGCACCATCGTTCTTGGCACTTTCGCCATCCAGATAGTGATCGTGCAGT
TTGGCGGGAAGCCCTTCAGCTGCTCCCCACTCCAGCTGGACCAGTGGATGTGGTGCATCTTCATAGGCCT
GGGAGAGCTCGTCTGGGGCCAGGTCATCGCCACCATCCCTACCAGCAGGCTCAAGTTCCTGAAAGAGGCA
GGGCGTCTAACACAGAAGGAGGAGATCCCCGAGGAGGAGTTAAATGAGGATGTGGAAGAGATAGACCACG
CAGAGCGGGAGCTTCGCCGTGGCCAGATCCTGTGGTTCCGGGGCCTGAATCGGATCCAGACACAGATCCG
CGTCGTGAAGGCGTTCCGTAGCTCTCTCTATGAAGGGTTAGAAAAACCCGAGTCTCGAACCTCCATCCAT
AACTTCATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATCGATGACACCG
ACCTGGAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCCTCGCTCAACAAGAACAATAGCGC
CATCGACAGCGGGATCAACCTGACGACCGACACGAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCC
ATCCACAGCCTGGAGACGTCGCTT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG223661 representing NM_001036684
Red=Cloning site Green=Tags(s)

MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPG
TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGESNEGCATAQGG
AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV
GDIAQIKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMVVTAVGVNS
QTGIIFTLLGAGGEEEEKKDKKAKQQDGAAAMEMQPLKSAEGGDADDKKKANMHKKEKSVLQGKLTKLAV
QIGKAGLVMSAITVIILVLYFTVDTFVVNKKPWLTECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTI
SLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINAKTL
ELLVNAIAINSAYTTKILPPEKEGALPRQVGNKTECGLLGFVLDLRQDYEPVRSQMPEEKLYKVYTFNSV
RKSMSTVIKMPDESFRMYSKGASEIVLKKCCKILSGAGEARVFRPRDRDEMVKKVIEPMACDGLRTICVA
YRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKC
GIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVV
AVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTV
NVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNI
LGHAVYQLTLIFTLLFVGEKMFQIDSGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDG
IFRNPIFCTIVLGTFAIQIVIVQFGGKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEA
GRLTQKEEIPEEELNEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIH
NFMAHPEFRIEDSQPHIPLIDDTDLEEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSP
IHSLETSL

TRTRPLE - GFP Tag - V
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001036684
ORF Size 3594 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001036684.3
RefSeq Size 6925 bp
RefSeq ORF 3597 bp
Locus ID 11941
UniProt ID Q9R0K7
Cytogenetics 6 52.85 cM
Gene Summary This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Plays a role in maintaining balance and hearing.[UniProtKB/Swiss-Prot Function]

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