Lpin1 (NM_172950) Mouse Tagged ORF Clone

CAT#: MG223610

Lpin1 (tGFP-tagged) - Mouse lipin 1 (Lpin1) transcript variant 1, (10ug)

Product Images

Circular map for MG223610

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lpin1
• Synonyms: 4631420P06; fld; Kiaa0188; Lipin1; mKIAA0188
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223610 representing NM_172950
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACGTGGGGCAGCTGGCCGGCCAGGTGTTTGTGACGGTGAAGGAGCTCTACAAGGGACTGAACC
  CCGCCACGCTGTCGGGATGCATCGACATCATTGTCATCCGGCAGCCCAATGGCAGTCTGCAGTGCTCCCC
  TTTCCACGTCCGCTTCGGCAAGATGGGTGTCCTCCGCTCCCGAGAGAAAGTGGTGGACATAGAAATCAAT
  GGGGAGTCCGTGGATTTGCACATGAAGTTGGGAGACAACGGAGAAGCATTTTTTGTTCAAGAGACTGACA
  ACGATCAGGAAATCATCCCCATGTACCTGGCCACGTCCCCCATCCTGTCAGAAGGAGCTGCGAGAATGGA
  AAGCCAGCTGAAGAGGAACTCTGTAGACAGAATCAGGTGCCTGGATCCCACTACAGCTGCCCAGGGCCTG
  CCTCCCAGCGACACCCCATCCACTGGTTCTCTGGGGAAGAAGAGAAGGAAAAGGAGGAGGAAGGCCCAGT
  TGGACAATCTCAAAAGAGATGACAATGTCAACACATCTGAGGATGAGGACATGTTTCCCATAGAGATGAG
  CTCGGATGAGGACACAGCACCGATGGATGGAAGCAGAACTCTTCCTAATGATGTACCACCATTCCAAGAT
  GATATTCCTAAGGAAAACTTCCCCTCGATTTCAACGTACCCCCAGTCAGCATCGTATCCCAGTTCGGACA
  GAGAATGGTCCCCCAGCCCCAGTCCTTCAGGCTCCCGGCCCTCAACACCAAAAAGTGACTCTGAGCTGGT
  CAGTAAGTCTGCAGACAGGTTGACGCCAAAGAATAACCTGGAAATGCTCTGGCTGTGGGGTGAATTGCCA
  CAGGCTGCAAAGTCCTCTTCTCCACACAAGATGAAAGAGTCCAGCCCCTTAGGGAGCCGGAAGACTCCTG
  ATAAAATGAATTTTCAGGCCATTCACAGCGAGTCTTCAGATACTTTTAGTGACCAGTCGCCAACAATGGC
  CCGGGGACTGCTCATCCACCAGAGTAAGGCCCAGACGGAAATGCAGTTTGTGAACGAGGAGGATCTCGAG
  TCCTTGGGGGCGGCAGCCCCACCTTCACCCGTGGCCGAAGAGCTCAAGGCCCCATATCCCAACACCGCAC
  AGTCGTCGAGCAAGACAGATTCCCCTTCCAGGAAGAAAGATAAACGGAGCCGACACCTTGGAGCTGATGG
  TGTTTATCTGGACGACCTCACGGACATGGACCCTGAAGTGGCAGCCCTGTATTTCCCCAAGAATGGGGAT
  CCTGGTGGGCTCCCCAAACAAGCCAGTGACAACGGAGCCAGGTCAGCCAACCAGTCACCACAGTCTGTGG
  GAGGCTCGGGCATCGACAGTGGTGTGGAGAGCACCTCCGACAGCCTGAGGGACCTGCCATCCATCGCCAT
  CTCCCTCTGCGGTGGCCTCAGTGACCACAGAGAGATCACCAAAGATGCATTTTTGGAACAAGCCGTGTCA
  TATCAGCAATTTGCCGACAACCCTGCTATCATCGATGACCCCAACCTCGTGGTCAAGGTTGGCAATAAGT
  ATTACAACTGGACAACAGCAGCTCCTCTACTTCTGGCGATGCAGGCTTTCCAGAAACCTTTGCCAAAGGC
  CACTGTGGAATCCATCATGAGAGATAAGATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCCTGGAGAGGA
  AGAAATGCCACAATCAAAGAGGAAAGCAAGCCTGAACAGTGCCTGACTGGGAAAGGCCACAATACCGGAG
  AGCAGCCTGCCCAGCTTGGCCTGGCCACCAGGATAAAGCATGAGTCATCCTCCAGTGATGAAGAGCACGC
  AGCCGCCAAGCCATCAGGTTCGAGCCACCTCTCTCTCTTGTCCAACGTCAGCTACAAAAAGACCCTGCGG
  CTCACGTCGGAGCAGCTGAAAAGCTTGAAGTTGAAGAACGGCCCCAATGATGTGGTGTTCAGTGTCACTA
  CCCAGTACCAGGGCACCTGTCGCTGCGAGGGCACCATCTACCTGTGGAATTGGGACGACAAAGTCATCAT
  CTCAGATATCGATGGGACCATCACAAGATCTGATACTCTTGGTCACATTTTGCCCACGCTGGGAAAGGAT
  TGGACTCACCAGGGCATTGCAAAGCTGTACCACAAAGTAAGCCAGAATGGCTACAAGTTTCTCTATTGTT
  CGGCACGTGCCATTGGGATGGCGGACATGACGAGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGT
  GCTTCCACAGGGCCCGCTTCTGCTCAGCCCGAGCAGCCTCTTCTCCGCCTTGCACAGAGAAGTGATTGAA
  AAGAAGCCAGAAAAGTTCAAAGTCCAGTGTTTGACAGACATCAAGAACCTGTTTTTCCCAAACACAGAAC
  CCTTCTATGCTGCTTTTGGGAACCGGCCTGCTGATGTGTATTCCTACAAGCAAGTGGGAGTGTCCCTGAA
  TAGGATCTTCACTGTGAACCCCAAGGGTGAGCTGGTGCAGGAGCATGCCAAGACCAACATCAGCTCGTAC
  GTGCGGCTCTGCGAAGTGGTCGATCACGTCTTCCCATTGCTAAAGAGAAGCCATTCCTGTGACTTCCCCT
  GTTCAGACACTTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGAAAACCAGGACATGCA
  TTCAGCCTCAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223610 representing NM_172950
  Red=Cloning site Green=Tags(s)
  
  MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEIN
  GESVDLHMKLGDNGEAFFVQETDNDQEIIPMYLATSPILSEGAARMESQLKRNSVDRIRCLDPTTAAQGL
  PPSDTPSTGSLGKKRRKRRRKAQLDNLKRDDNVNTSEDEDMFPIEMSSDEDTAPMDGSRTLPNDVPPFQD
  DIPKENFPSISTYPQSASYPSSDREWSPSPSPSGSRPSTPKSDSELVSKSADRLTPKNNLEMLWLWGELP
  QAAKSSSPHKMKESSPLGSRKTPDKMNFQAIHSESSDTFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLE
  SLGAAAPPSPVAEELKAPYPNTAQSSSKTDSPSRKKDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGD
  PGGLPKQASDNGARSANQSPQSVGGSGIDSGVESTSDSLRDLPSIAISLCGGLSDHREITKDAFLEQAVS
  YQQFADNPAIIDDPNLVVKVGNKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRG
  RNATIKEESKPEQCLTGKGHNTGEQPAQLGLATRIKHESSSSDEEHAAAKPSGSSHLSLLSNVSYKKTLR
  LTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKD
  WTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIE
  KKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSY
  VRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREPLPPFENQDMHSASA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172950
• ORF Size: 2673 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172950.3, NP_766538.2
• RefSeq Size: 5482 bp
• RefSeq ORF: 2676 bp
• Locus ID: 14245
• UniProt ID: Q91ZP3
• Cytogenetics: 12 7.9 cM
• Gene Summary: Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.[UniProtKB/Swiss-Prot Function]