Sept9 (NM_001113487) Mouse Tagged ORF Clone

CAT#: MG223360

41526 (tGFP-tagged) - Mouse septin 9 (Sept9) transcript variant 2, (10ug)

Product Images

Circular map for MG223360

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sept9
• Synonyms: Msf; MSF1; PNUTL4; Sint1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223360 representing NM_001113487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGACCCCGCGGTCAACGCACAGCTGGACGGGATCATTTCAGACTTTGAAGCCTTAAAGAGATCGT
  TTGAAGTCGAGGAGATTGAGCCGCCCAACTCCACGCCACCCCGGAGGGTCCAGACCCCTCTGCTCCGCGC
  CACGGTGGCCAGCTCCAGCCAGAAATTCCAGGACCTGGGGGTGAAGAACTCAGAGCCCGCTGCCCGCCTT
  GTAGACTCCCTGAGCCAGCGCTCCCCCAAGCCTTCCCTGCGGAGGGTGGAGCTGGCGGGAGCCAAGGCGC
  CTGAGCCCATGTCTCGACGCACAGAGATCTCCATCGACATCTCCTCCAAGCAGGTGGAGAGCACGGCCTC
  CGCTGCCGGGCCCTCACGGTTCGGCCTTAAGAGGGCTGAAGTCCTGGGCCACAAGACCCCAGAGCCTGTC
  CCTCGGAGGACGGAGATCACCATTGTCAAGCCTCAGGAGTCAGTGCTCCGCAGGGTGGAGACCCCTGCCT
  CCAAGATTCCCGAGGGCTCTGCCGTGCCTGCCACAGATGCAGCCCCCAAGAGGGTAGAGATCCAGGTGCC
  CAAGCCAGCTGAGGCACCCAACTGTCCGCTCCCGTCCCAGACCCTGGAGAACTCCGAGGCCCCGATGTCT
  CAACTGCAGAGCAGGCTGGAGCCCAGGCCTTCCGTGGCTGAGGTCCCATATCGGAACCAGGAAGACTCCG
  AGGTGACTCCCAGCTGTGTTGGCGACATGGCTGACAACCCTAGAGATGCCATGCTCAAGCAGGCCCCCGC
  GTCACGGAACGAAAAGGCCCCCATGGAGTTCGGCTATGTGGGGATCGACTCCATCCTGGAGCAGATGCGC
  AGGAAGGCTATGAAACAGGGCTTCGAGTTTAACATCATGGTGGTTGGGCAGAGCGGCCTCGGGAAGTCCA
  CTTTAATCAATACCCTCTTCAAGTCCAAAATCAGCCGGAAGTCGGTGCAGCCCACCTCGGAGGAACGCAT
  CCCCAAGACGATCGAAATCAAGTCGATCACCCACGATATTGAAGAGAAGGGGGTTCGAATGAAGCTGACA
  GTGATTGACACGCCGGGCTTCGGAGACCACATCAACAATGAGAACTGCTGGCAGCCCATTATGAAGTTCA
  TCAATGACCAATATGAGAAGTACCTGCAGGAGGAAGTCAACATCAACCGGAAGAAGCGCATTCCCGACAC
  CCGTGTCCACTGCTGCCTCTACTTCATCCCAGCCACCGGCCACTCGCTCAGGCCCCTGGACATTGAATTC
  ATGAAGCGCCTAAGCAAAGTGGTGAACATTGTCCCAGTCATCGCCAAGGCTGACACGCTGACCCTGGAGG
  AGAGGGTCTACTTCAAACAGCGGATCACTGCAGACCTGCTGTCCAACGGCATTGACGTGTACCCGCAGAA
  GGAGTTTGATGAGGACGCAGAAGACCGGCTGGTGAACGAAAAGTTCCGGGAGATGATCCCATTTGCTGTG
  GTGGGCAGCGACCATGAGTATCAAGTCAATGGCAAGAGGATTCTGGGAAGGAAGACCAAGTGGGGCACTA
  TCGAAGTTGAGAATACCACTCACTGTGAATTTGCTTACCTGCGGGATCTCCTTATCAGGACGCACATGCA
  AAACATCAAAGACATCACCAGCAACATCCACTTCGAAGCCTACCGAGTGAAACGCCTCAACGAGGGCAAC
  AGCGCCATGGCCAACGGGATCGAGAAGGAGCCGGAAGCCCAGGAGATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223360 representing NM_001113487
  Red=Cloning site Green=Tags(s)
  
  MSDPAVNAQLDGIISDFEALKRSFEVEEIEPPNSTPPRRVQTPLLRATVASSSQKFQDLGVKNSEPAARL
  VDSLSQRSPKPSLRRVELAGAKAPEPMSRRTEISIDISSKQVESTASAAGPSRFGLKRAEVLGHKTPEPV
  PRRTEITIVKPQESVLRRVETPASKIPEGSAVPATDAAPKRVEIQVPKPAEAPNCPLPSQTLENSEAPMS
  QLQSRLEPRPSVAEVPYRNQEDSEVTPSCVGDMADNPRDAMLKQAPASRNEKAPMEFGYVGIDSILEQMR
  RKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLT
  VIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEF
  MKRLSKVVNIVPVIAKADTLTLEERVYFKQRITADLLSNGIDVYPQKEFDEDAEDRLVNEKFREMIPFAV
  VGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSNIHFEAYRVKRLNEGN
  SAMANGIEKEPEAQEM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001113487
• ORF Size: 1728 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113487.1, NP_001106959.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 1731 bp
• Locus ID: 53860
• UniProt ID: Q80UG5
• Cytogenetics: 11 82.61 cM
• Gene Summary: Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential).[UniProtKB/Swiss-Prot Function]