Psmb9 (NM_013585) Mouse Tagged ORF Clone

CAT#: MG223033

Psmb9 (tGFP-tagged) - Mouse proteasome (prosome macropain) subunit beta type 9 (large multifunctional peptidase 2) (Psmb9), (10ug)

Product Images

Circular map for MG223033

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Psmb9
• Synonyms: Lmp-2; Lmp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223033 representing NM_013585
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCGGGCAGGAGCACCTACCGCCGGCTCGTTCCGGACGGAAGAAGTCCACACCGGGACAACCATCA
  TGGCAGTGGAGTTTGACGGGGGTGTCGTGGTGGGCTCTGATTCCCGGGTGTCAGCGGGAACAGCAGTGGT
  GAACCGCGTGTTCGACAAGCTCTCCCCTCTGCACCAGCACATCTTCTGTGCCCTCTCAGGTTCCGCTGCT
  GATGCCCAAGCCATAGCTGACATGGCCGCCTACCAGCTGGAGCTACACGGGTTGGAGCTGGAGGAGCCGC
  CCCTCGTTCTGGCTGCTGCAAACGTGGTGAAGAACATCTCCTACAAGTACCGTGAGGACTTGTTAGCGCA
  TCTCATAGTAGCTGGCTGGGACCAATGTGAGGGGGGACAGGTATATGGAACCATGGGAGGGATGCTAATT
  CGACAGCCCTTTACCATCGGCGGTTCCGGAAGCTCCTACATTTATGGTTATGTGGACGCAGCTTATAAGC
  CAGGCATGACCCCTGAGGAGTGCCGGCGTTTCACCACAGATGCCATCACTCTGGCCATGAACCGAGATGG
  CTCTAGTGGGGGTGTCATCTACCTGGTCACCATCACAGCTGCTGGTGTGGACCATCGAGTCATCCTGGGA
  GATGAGCTGCCAAAATTCTACGATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223033 representing NM_013585
  Red=Cloning site Green=Tags(s)
  
  MLRAGAPTAGSFRTEEVHTGTTIMAVEFDGGVVVGSDSRVSAGTAVVNRVFDKLSPLHQHIFCALSGSAA
  DAQAIADMAAYQLELHGLELEEPPLVLAAANVVKNISYKYREDLLAHLIVAGWDQCEGGQVYGTMGGMLI
  RQPFTIGGSGSSYIYGYVDAAYKPGMTPEECRRFTTDAITLAMNRDGSSGGVIYLVTITAAGVDHRVILG
  DELPKFYDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013585
• ORF Size: 657 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013585.2, NP_038613.1
• RefSeq Size: 806 bp
• RefSeq ORF: 660 bp
• Locus ID: 16912
• UniProt ID: P28076
• Cytogenetics: 17 17.98 cM
• Gene Summary: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Contributes to NFKBIA degradation and subsequently NFKB1 generation.[UniProtKB/Swiss-Prot Function]