Gata6 (NM_010258) Mouse Tagged ORF Clone

CAT#: MG222974

Gata6 (tGFP-tagged) - Mouse GATA binding protein 6 (Gata6), (10ug)

Product Images

Circular map for MG222974

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gata6
• Synonyms: AA410133; GATA-6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222974 representing NM_010258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGACTGACGGCGGCTGGTGCCTGCCAAAGCGTTTCGGGGCTGCTGCTGCGGACGCCGGCGACT
  CCGGGCCCTTTCCAGCGCGGGAGCCCTCCTCGCCGCTTTCCCCCATCTCGTCTTCGTCCTCCTCCTGCTC
  CCGGGGCGGGGATCGCGGTCCCTGCGGCGCCAGCAACTGCAGGACGCCGCAGCTCGACGCCGAGGCGGTG
  GCGGGACCTCCGGGCCGCTCGCTCTTGCTCAGCCCCTACGCCTCGCATCCCTTCGCCGCTGCCCACGGAG
  CCGCGGCGCCCGGGGTCGCAGGCCCCGGGAGCGCCCTGTCGACTTGGGAGGACCTGTTGCTCTTCACTGA
  CCTCGATCAGGCCGCGACCGCCAGCAAGCTGTTGTGGTCCAGCCGGGGCGCCAAACTGAGCCCCTTCGCG
  GCCGAGCAGCCGGAGGAAATGTACCAGACCCTCGCCGCCCTGTCCAGCCAGGGGCCCGCCGCTTACGACG
  GCGCGCCCGGCGGCTTCGTGCACTCCGCAGCGGCGGCGGCCGCTGCCGCCGCGGCAGCCAGCTCCCCGGT
  CTACGTGCCCACCACGCGCGTGGGCTCCATGCTGTCCGGCCTGCCCTACCTTCAAGGGGCGGGCAGCGGG
  CCCAGCAATCACGCGGGCGGAGCGGGTGCCCACCCAGGCTGGTCCCAGGCCTCCGCCGACAGCCCCCCGT
  ATGGCGGGGGTGGCGCAGCCGGCGGCGGCGCGGCCGGACCTGGAGGTGCGGGATCGGCTACGGCCCACGC
  CTCTGCACGCTTTCCCTACTCGCCCAGCCCGCCCATGGCCAACGGCGCCGCGCGAGACCCCGGGGGCTAC
  GTGGCTGCGGGCGGCACGGGCGCAGGCAGTGTGAGTGGAGGTGGCGGCAGCCTGGCGGCCATGGGTGGCC
  GGGAGCACCAGTACAGCTCGCTGTCCGCAGCTCGGCCGCTGAACGGAACGTACCACCACCACCATCACCA
  TCACCCGACCTACTCGCCCTACATGGCCGCACCGCTGACTCCTGCCTGGCCAGCAGGACCCTTCGAAACG
  CCGGTGCTCCACAGCTTACAGGGCCGCGCGGGAGCTCCACTCCCGGTGCCACGGGGCCCCAGCACAGACC
  TGTTGGAGGACCTGTCGGAGAGCCGCGAGTGCGTGAACTGCGGCTCCATCCAGACGCCACTGTGGAGACG
  AGACGGCACCGGTCATTACCTGTGCAATGCATGCGGTCTCTACAGCAAGATGAATGGCCTCAGCAGGCCC
  CTCATCAAGCCACAGAAGCGCGTGCCTTCATCACGGCGGCTTGGACTGTCCTGTGCCAACTGTCACACCA
  CAACCACTACCTTATGGCGTAGAAATGCTGAGGGTGAGCCTGTGTGCAATGCTTGCGGGCTCTATATGAA
  ACTCCATGGGGTGCCTCGACCACTTGCTATGAAAAAAGAAGGAATTCAAACCAGGAAACGAAAACCTAAA
  AATATAAATAAGTCAAAAGCTTGCTCCGGTAACAGCAGTGGCTCTGTCCCTATGACTCCTACTTCCTCTT
  CTTCTAATTCAGATGACTGCACCAAAAATACTTCTCCTTCTACACAAGCGACCACCTCAGGGGTAGGGGC
  ATCAGTGATGTCTGCAGTGGGAGAAAACGCCAACCCCGAGAACAGTGACCTCAAGTATTCAGGTCAAGAC
  GGCCTCTACATAGGTGTCAGTCTGTCCTCCCCTGCCGAAGTCACATCCTCCGTGCGACAGGATTCTTGGT
  GTGCTCTGGCCCTGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222974 representing NM_010258
  Red=Cloning site Green=Tags(s)
  
  MALTDGGWCLPKRFGAAAADAGDSGPFPAREPSSPLSPISSSSSSCSRGGDRGPCGASNCRTPQLDAEAV
  AGPPGRSLLLSPYASHPFAAAHGAAAPGVAGPGSALSTWEDLLLFTDLDQAATASKLLWSSRGAKLSPFA
  AEQPEEMYQTLAALSSQGPAAYDGAPGGFVHSAAAAAAAAAAASSPVYVPTTRVGSMLSGLPYLQGAGSG
  PSNHAGGAGAHPGWSQASADSPPYGGGGAAGGGAAGPGGAGSATAHASARFPYSPSPPMANGAARDPGGY
  VAAGGTGAGSVSGGGGSLAAMGGREHQYSSLSAARPLNGTYHHHHHHHPTYSPYMAAPLTPAWPAGPFET
  PVLHSLQGRAGAPLPVPRGPSTDLLEDLSESRECVNCGSIQTPLWRRDGTGHYLCNACGLYSKMNGLSRP
  LIKPQKRVPSSRRLGLSCANCHTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMKKEGIQTRKRKPK
  NINKSKACSGNSSGSVPMTPTSSSSNSDDCTKNTSPSTQATTSGVGASVMSAVGENANPENSDLKYSGQD
  GLYIGVSLSSPAEVTSSVRQDSWCALALA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010258
• ORF Size: 1767 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010258.3, NP_034388.2
• RefSeq Size: 3194 bp
• RefSeq ORF: 1770 bp
• Locus ID: 14465
• UniProt ID: Q61169
• Cytogenetics: 18 5.63 cM
• Gene Summary: Transcriptional activator that regulates SEMA3C and PLXNA2 (PubMed:19666519). May regulate genes that protect epithelial cells from bacterial infection (By similarity). Involved in gene regulation specifically in the gastric epithelium (By similarity). Involved in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (PubMed:15329343).[UniProtKB/Swiss-Prot Function]