Eng (NM_007932) Mouse Tagged ORF Clone

CAT#: MG222686

Eng (tGFP-tagged) - Mouse endoglin (Eng) transcript variant 1, (10ug)

Product Images

Circular map for MG222686

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eng
• Synonyms: AI528660; AI662476; CD105; Endo; S-endoglin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222686 representing NM_007932
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCGTGGCGTGCTCCCTCTGCCCATTACCCTGCTGTTTGTCATCTATAGCTTTGTACCCACAACAG
  GTCTCGCAGAAAGAGTCGGCTGTGATCTACAGCCTGTGGACCCCACAAGGGGTGAGGTGACGTTTACCAC
  CAGCCAGGTCTCCGAGGGCTGTGTAGCTCAGGCTGCCAATGCTGTGCGTGAAGTCCACGTTCTCTTCCTG
  GATTTTCCCGGAATGCTGTCACATCTGGAGCTGACTCTTCAGGCATCCAAGCAAAATGGCACGGAGACCC
  AGGAGGTGTTCCTGGTCCTCGTTTCGAACAAAAATGTCTTCGTGAAGTTCCAGGCCCCGGAAATCCCATT
  GCACTTGGCCTACGACTCCAGCCTGGTCATCTTCCAAGGACAGCCAAGAGTCAACATCACAGTGCTACCA
  TCCCTTACCTCCAGGAAACAGATCCTCGACTGGGCAGCCACCAAGGGCGCCATCACCTCGATAGCAGCAC
  TGGATGACCCCCAAAGCATCGTCCTCCAGTTGGGCCAAGACCCAAAGGCACCATTCTTGTGCTTGCCAGA
  AGCTCACAAGGACATGGGCGCCACACTTGAATGGCAACCACGAGCCCAGACCCCAGTCCAAAGCTGTCGC
  TTGGAAGGTGTGTCTGGCCACAAGGAGGCCTACATCCTGAGGATCCTGCCAGGTTCTGAGGCCGGGCCCC
  GGACGGTGACCGTAATGATGGAACTGAGTTGCACATCTGGGGACGCCATTCTCATCCTGCATGGTCCTCC
  ATATGTCTCCTGGTTCATCGACATCAACCACAGCATGCAGATCTTGACCACAGGTGAATACTCCGTCAAG
  ATCTTTCCAGGAAGCAAGGTCAAAGGCGTGGAGCTCCCAGACACACCCCAAGGCCTGATAGCGGAGGCCC
  GCAAGCTCAATGCCAGCATTGTCACCTCCTTTGTAGAGCTCCCTCTGGTCAGCAATGTCTCCCTGAGGGC
  CTCCAGCTGCGGTGGTGTGTTCCAGACCACCCCTGCACCCGTTGTGACCACACCTCCCAAGGACACATGC
  AGCCCCGTGCTACTCATGTCCCTGATCCAGCCAAAGTGTGGCAATCAGGTCATGACTCTGGCACTCAATA
  AAAAACACGTGCAGACTCTCCAGTGCACCATCACAGGCCTGACTTTCTGGGACTCCAGCTGCCAGGCTGA
  AGACACTGACGACCATCTTGTCCTGAGTAGCGCCTACTCCAGCTGCGGCATGAAAGTGACAGCCCATGTG
  GTCAGCAATGAGGTGATCATCAGTTTCCCGTCAGGCTCACCACCACTTCGGAAAAAGGTACAGTGCATCG
  ACATGGACAGCCTCTCCTTCCAGCTGGGCCTCTACCTCAGCCCGCACTTCCTCCAGGCATCCAACACCAT
  CGAACTAGGCCAGCAGGCCTTCGTACAGGTGAGCGTGTCTCCATTGACCTCTGAGGTCACAGTCCAGCTA
  GATAGCTGCCATCTGGACTTGGGGCCCGAAGGGGACATGGTGGAACTCATCCAGAGCCGAACAGCCAAGG
  GCAGCTGTGTGACCTTGCTGTCTCCAAGCCCTGAAGGTGACCCACGCTTCAGCTTCCTCCTCCGGGTCTA
  CATGGTGCCCACACCCACCGCTGGCACCCTCAGTTGCAACTTAGCTCTGCGCCCTAGCACCTTGTCCCAG
  GAAGTCTACAAGACAGTCTCCATGCGCCTGAACATCGTCAGCCCTGACCTGTCTGGTAAAGGCCTTGTCC
  TGCCCTCTGTACTGGGTATCACCTTTGGTGCCTTCCTGATTGGGGCCCTGCTCACAGCTGCACTCTGGTA
  CATCTATTCTCACACACGTGGCCCCAGCAAGCGGGAGCCCGTGGTGGCAGTGGCTGCCCCGGCCTCCTCT
  GAGAGCAGCAGTACCAACCACAGCATCGGGAGCACCCAGAGCACCCCCTGCTCCACCAGCAGCATGGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222686 representing NM_007932
  Red=Cloning site Green=Tags(s)
  
  MDRGVLPLPITLLFVIYSFVPTTGLAERVGCDLQPVDPTRGEVTFTTSQVSEGCVAQAANAVREVHVLFL
  DFPGMLSHLELTLQASKQNGTETQEVFLVLVSNKNVFVKFQAPEIPLHLAYDSSLVIFQGQPRVNITVLP
  SLTSRKQILDWAATKGAITSIAALDDPQSIVLQLGQDPKAPFLCLPEAHKDMGATLEWQPRAQTPVQSCR
  LEGVSGHKEAYILRILPGSEAGPRTVTVMMELSCTSGDAILILHGPPYVSWFIDINHSMQILTTGEYSVK
  IFPGSKVKGVELPDTPQGLIAEARKLNASIVTSFVELPLVSNVSLRASSCGGVFQTTPAPVVTTPPKDTC
  SPVLLMSLIQPKCGNQVMTLALNKKHVQTLQCTITGLTFWDSSCQAEDTDDHLVLSSAYSSCGMKVTAHV
  VSNEVIISFPSGSPPLRKKVQCIDMDSLSFQLGLYLSPHFLQASNTIELGQQAFVQVSVSPLTSEVTVQL
  DSCHLDLGPEGDMVELIQSRTAKGSCVTLLSPSPEGDPRFSFLLRVYMVPTPTAGTLSCNLALRPSTLSQ
  EVYKTVSMRLNIVSPDLSGKGLVLPSVLGITFGAFLIGALLTAALWYIYSHTRGPSKREPVVAVAAPASS
  ESSSTNHSIGSTQSTPCSTSSMA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007932
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007932.2, NP_031958.2
• RefSeq Size: 3433 bp
• RefSeq ORF: 1962 bp
• Locus ID: 13805
• UniProt ID: Q63961
• Cytogenetics: 2 22.09 cM
• Gene Summary: Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed:10625534). Required for normal structure and integrity of adult vasculature (By similarity). Regulates the migration of vascular endothelial cells (PubMed:17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (PubMed:10625534). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (PubMed:28530658). May play a role in the binding of endothelial cells to integrins. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed:23300529). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (By similarity).[UniProtKB/Swiss-Prot Function]