Hspd1 (NM_010477) Mouse Tagged ORF Clone

CAT#: MG222671

Hspd1 (tGFP-tagged) - Mouse heat shock protein 1 (chaperonin) (Hspd1) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG222671

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hspd1
• Synonyms: 60kDa; CPN60; HSP-60; HSP-65; Hsp60
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222671 representing NM_010477
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCGACTACCCACAGTCCTTCGCCAGATGAGACCAGTGTCCCGGGCACTGGCTCCTCATCTCACTC
  GGGCCTATGCCAAAGATGTAAAATTTGGTGCGGACGCTCGAGCCTTAATGCTTCAAGGTGTAGACCTTTT
  AGCAGATGCTGTAGCTGTTACAATGGGGCCAAAGGGAAGAACTGTGATTATTGAACAGAGTTGGGGAAGT
  CCCAAAGTAACAAAAGATGGGGTCACTGTTGCAAAGTCAATTGATTTAAAGGATAAATACAAAAATATTG
  GAGCTAAACTTGTTCAGGACGTTGCCAATAACACAAACGAAGAGGCTGGGGATGGCACCACCACTGCCAC
  TGTTCTGGCACGATCTATTGCCAAGGAGGGCTTTGAGAAGATCAGCAAAGGGGCTAATCCAGTGGAAATC
  CGGAGAGGTGTGATGTTGGCTGTGGATGCTGTAATTGCTGAACTTAAGAAACAGTCTAAACCTGTGACAA
  CCCCTGAAGAAATTGCTCAGGTTGCTACAATTTCTGCAAATGGAGACAAAGACATTGGGAACATCATTTC
  TGATGCAATGAAAAAGGTTGGAAGAAAGGGTGTCATCACAGTGAAGGATGGAAAAACCCTGAATGATGAG
  CTAGAAATTATTGAAGGCATGAAGTTTGATAGAGGATATATTTCCCCGTATTTTATTAACACATCAAAAG
  GTCAAAAGTGTGAATTCCAAGATGCCTATGTCTTGTTGAGTGAAAAGAAAATTTCCAGTGTTCAGTCCAT
  TGTCCCTGCTCTTGAAATTGCTAATGCTCATCGGAAGCCATTGGTCATAATCGCCGAAGACGTTGACGGA
  GAAGCTCTAAGCACGCTGGTTTTGAACAGGCTAAAAGTTGGTCTTCAGGTTGTGGCAGTCAAAGCTCCAG
  GATTTGGGGACAATAGGAAGAACCAGCTTAAAGATATGGCTATTGCTACTGGTGGTGCAGTGTTTGGAGA
  AGAGGGGTTGAATCTAAATCTTGAAGATGTTCAAGCTCATGACTTAGGAAAAGTTGGGGAGGTCATTGTC
  ACCAAAGATGATGCCATGCTTTTGAAAGGAAAAGGTGACAAAGCTCACATTGAAAAACGTATTCAAGAAA
  TCACTGAGCAGCTAGACATCACAACTAGTGAATATGAAAAAGAAAAGCTGAACGAGCGACTTGCTAAACT
  TTCAGATGGAGTAGCTGTGTTGAAGGTTGGAGGAACAAGTGATGTTGAAGTGAATGAGAAAAAAGACAGA
  GTTACTGATGCTCTCAATGCTACAAGAGCAGCTGTTGAAGAAGGCATTGTTCTAGGAGGGGGCTGCGCTC
  TGCTTCGGTGCATCCCAGCCTTGGATTCATTAAAGCCTGCTAATGAAGACCAGAAAATAGGTATAGAAAT
  TATTAAAAGAGCACTTAAAATTCCTGCAATGACGATTGCTAAGAATGCAGGTGTTGAAGGATCTTTGATA
  GTTGAGAAAATTCTGCAGAGTTCCTCAGAAGTTGGTTATGACGCCATGCTTGGAGATTTTGTGAACATGG
  TGGAAAAAGGGATCATTGATCCAACAAAGGTTGTGAGAACTGCCTTACTGGATGCTGCTGGGGTGGCCTC
  CTTGCTAACTACAGCCGAAGCTGTAGTGACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA
  ATGGGTGGCATGGGAGGGGGTATGGGAGGCGGCATGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222671 representing NM_010477
  Red=Cloning site Green=Tags(s)
  
  MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGS
  PKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEI
  RRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDE
  LEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDG
  EALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIV
  TKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDR
  VTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLI
  VEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGA
  MGGMGGGMGGGMF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010477
• ORF Size: 1719 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010477.4, NP_034607.3
• RefSeq Size: 2347 bp
• RefSeq ORF: 1722 bp
• Locus ID: 15510
• UniProt ID: P63038
• Cytogenetics: 1 C1.2
• Gene Summary: Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.[UniProtKB/Swiss-Prot Function]