Cps1 (NM_001080809) Mouse Tagged ORF Clone

CAT#: MG222149

Cps1 (tGFP-tagged) - Mouse carbamoyl-phosphate synthetase 1 (Cps1) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG222149

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cps1
• Synonyms: 4732433M03Rik; C; CPS; D1Ucl; D1Ucla3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222149 representing NM_001080809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGAGGATTTTGACAGCTTGCAAAGTGGTGAAGACCCTGAAGAGTGGCTTTGGTTTTGCCAATGTGA
  CTACGAAGCGACAGTGGGACTTCTCCAGACCTGGCATCAGGCTCCTTTCTGTGAAGGCAAAGACAGCACA
  CATTGTTCTGGAAGATGGAACTAAGATGAAAGGCTACTCCTTTGGCCATCCCTCCTCTGTTGCTGGTGAA
  GTGGTTTTTAATACTGGCTTAGGAGGGTACCCGGAAGCACTTACTGATCCCGCCTACAAGGGGCAGATCC
  TCACCATGGCCAACCCTATCATTGGGAACGGTGGGGCTCCCGACACCACTGCCCGAGACGAACTGGGACT
  GAATAAGTACATGGAGTCTGATGGGATCAAGGTGGCAGGTCTGCTGGTTCTGAATTACAGTAATGACTAT
  AACCACTGGCTGGCTACCAAGAGTCTGGGTCAATGGCTGCAGGAGGAAAAGGTCCCTGCAATTTATGGAG
  TGGATACAAGAATGCTGACTAAAATAATTCGGGATAAGGGTACCATGCTTGGGAAGATTGAATTTGAAGG
  CCAGTCTGTGGATTTTGTGGATCCCAATAAGCAGAATTTGATTGCTGAGGTTTCAACCAAGGATGTCAAG
  GTGTTTGGCAAAGGAAACCCCACGAAAGTGGTAGCTGTGGACTGTGGGATAAAAAACAATGTCATCCGCC
  TGCTAGTTAAGCGAGGAGCTGAAGTGCATTTGGTTCCCTGGAATCACGACTTCACCCAGATGGAGTATGA
  TGGACTTTTGATTGCTGGGGGACCTGGGAATCCAGCTCTTGCACAGCCACTAATTCAGAATGTGAAAAAG
  ATTTTGGAGAGTGACCGCAAAGAGCCATTGTTTGGAATCAGTACAGGAAACATAATAACAGGATTGGCTG
  CTGGTGCCAAATCCTACAAGATGTCCATGGCCAACAGAGGACAGAACCAACCTGTTTTGAATATCACAAA
  CAGACAGGCTTTCATAACTGCTCAGAATCATGGCTATGCTCTGGACAACACACTCCCTGCTGGCTGGAAA
  CCACTGTTTGTGAATGTCAATGATCAAACAAACGAGGGGATTATGCATGAGAGCAAACCTTTCTTCGCTG
  TGCAGTTCCACCCAGAGGTCAGCCCGGGGCCAACAGACACTGAGTACCTATTTGATTCCTTCTTCTCCCT
  GATAAAGAAGGGCAAAGGCACCACCATTACCTCAGTTCTGCCCAAGCCAGCGTTGGTGGCATCTCGTGTC
  GAGGTTTCCAAGGTCCTCATCCTGGGATCAGGAGGTCTGTCCATTGGTCAGGCAGGTGAATTTGATTACT
  CGGGATCTCAAGCTGTGAAAGCCATGAAGGAAGAAAATGTCAAAACAGTCCTGATGAACCCGAACATCGC
  ATCCGTGCAGACCAACGAGGTGGGATTGAAGCAGGCAGATGCAGTCTACTTTCTCCCTATTACCCCCCAG
  TTTGTCACAGAAGTTATCAAGGCTGAACGGCCTGATGGGCTGATTCTGGGCATGGGTGGCCAGACAGCTC
  TGAACTGTGGAGTGGAACTATTCAAGAGGGGTGTGCTCAAGGAATATGGGGTGAAGGTCTTGGGCACATC
  AGTTGAGTCCATTATGGCCACAGAAGACAGGCAGCTGTTCTCAGACAAGCTGAATGAGATCAACGAGAAG
  ATCGCACCCAGTTTTGCAGTGGAATCAATGGAGGATGCCTTGAAGGCAGCAGACACCATTGGCTACCCTG
  TGATGATTCGGTCTGCATATGCTCTGGGTGGCTTAGGCTCTGGCATCTGTCCCAATAAGGAGACCTTAAT
  AGACCTTGGCACAAAAGCATTTGCTATGACCAACCAGATCCTGGTGGAGCGGTCAGTGACAGGTTGGAAA
  GAAATAGAATATGAAGTTGTCCGAGATGCAGATGATAACTGTGTCACAGTCTGTAACATGGAAAATGTTG
  ACGCCATGGGTGTTCACACAGGTGACTCGGTTGTCGTGGCCCCAGCCCAGACACTCTCCAATGCAGAGTT
  CCAGATGTTGAGACGCACTTCAGTCAATGTTGTTCGTCACTTGGGCATTGTGGGCGAATGCAACATTCAG
  TTTGCTCTTCACCCCACTTCCATGGAATACTGTATCATTGAAGTGAATGCCAGGCTGTCCCGGAGTTCTG
  CCCTGGCCTCCAAGGCCACTGGCTACCCACTGGCATTCATTGCAGCAAAGATTGCTCTAGGAATCCCACT
  TCCAGAAATCAAGAATGTTGTATCTGGGAAGACCTCAGCCTGCTTCGAACCTAGCCTGGATTACATGGTG
  ACCAAGATTCCTCGCTGGGATCTTGACCGTTTCCATGGAACATCCAGCCGAATTGGTAGCTCAATGAAAA
  GTGTGGGCGAGGTCATGGCCATTGGTCGCACCTTTGAGGAAAGTTTCCAGAAGGCTCTGAGGATGTGCCA
  TCCATCTGTGGATGGGTTCACTCCCCGGCTCCCAATGAATAAGGAATGGCCAGCAAACCTGGATCTGAAG
  AAAGAGCTGTCTGAGCCCTCCAGCACCCGCATCTACGCCATTGCTAAGGCCTTGGAGAACAACATGTCCC
  TCGACGAAATTGTGAGGCTCACGTCCATCGACAAGTGGTTTCTGTACAAGATGCGTGACATTTTAAACAT
  GGATAAGACACTGAAAGGGCTTAACAGTGACTCTGTTACAGAAGAAACTCTGAGAAAGGCCAAGGAGATT
  GGGTTCTCAGACAAGCAGATTTCAAAATGTTTGGGACTGACTGAAGCCCAGACAAGAGAGCTGAGGTTAA
  AGAAAAACATCCACCCTTGGGTTAAACAGATTGATACATTGGCTGCAGAATACCCATCAGTAACAAACTA
  CCTGTATGTTACCTACAACGGCCAGGAGCACGATATCAAATTTGATGAACATGGAATAATGGTTCTGGGC
  TGTGGTCCATACCACATTGGCAGCAGTGTGGAATTTGATTGGTGTGCTGTCTCCAGTATCCGTACACTGC
  GCCAACTTGGCAAGAAGACAGTGGTGGTGAATTGTAACCCAGAGACTGTGAGCACTGATTTTGATGAGTG
  TGACAAACTCTACTTTGAAGAGCTGTCTTTGGAGAGAATCCTAGACATCTACCACCAGGAGGCATGTAAT
  GGCTGCATCATATCAGTTGGCGGCCAGATTCCAAACAACTTGGCGGTCCCGCTGTACAAGAATGGTGTCA
  AGATCATGGGTACAAGCCCTCTGCAGATCGACAGGGCTGAGGATCGCTCCATCTTCTCAGCTGTCTTAGA
  TGAGCTAAAGGTGGCCCAGGCTCCATGGAAAGCTGTGAACACTTTGAATGAAGCACTGGAATTTGCAAAC
  TCTGTGGGCTACCCCTGCTTACTGAGACCTTCCTATGTTTTGAGTGGGTCTGCCATGAACGTGGTATTCT
  CTGAGGATGAGATGAAAAGGTTTCTTGAGGAGGCCACTCGAGTCTCTCAGGAACACCCGGTGGTGCTGAC
  CAAGTTTGTGGAGGGGGCCCGGGAAGTAGAGATGGACGCTGTTGGCAAAGAAGGACGGGTCATCTCCCAT
  GCCATCTCTGAACATGTTGAAGATGCAGGTGTCCACTCAGGAGATGCCACCCTGATGCTACCTACACAGA
  CCATCAGCCAAGGAGCCATTGAAAAGGTGAAGGATGCCACACGAAAGATTGCAAAAGCCTTTGCCATCTC
  TGGGCCATTCAATGTCCAGTTTCTTGTCAAAGGAAATGATGTCTTGGTGATTGAGTGCAATCTGAGAGCA
  TCAAGATCCTTCCCCTTTGTCTCCAAGACTCTTGGGGTGGACTTCATTGACGTGGCTACAAAGGTGATGA
  TCGGGGAGAGCATTGATGAGAAGCGACTTCCCACGCTGGAGCAGCCCATCATTCCCTCTGACTATGTTGC
  CATTAAGGCTCCAATGTTTTCCTGGCCCCGACTGAGAGATGCTGACCCTATTCTGAGATGTGAGATGGCT
  TCTACTGGAGAGGTGGCGTGCTTCGGCGAGGGCATTCATACCGCCTTCCTAAAGGCCATGCTGTCTACAG
  GGTTTAAGATACCTCAGAAGGGCATTCTGATTGGAATCCAGCAATCATTCCGTCCAAGATTCCTTGGTGT
  TGCTGAGCAGTTGCACAATGAAGGTTTCAAGCTTTTTGCCACAGAAGCTACATCAGACTGGCTCAATGCC
  AACAATGTCCCTGCCACCCCTGTGGCTTGGCCATCTCAGGAAGGACAGAATCCCAGCCTCTCTTCCATCA
  GAAAGTTGATAAGAGATGGAAGCATTGACCTAGTGATTAACCTCCCCAATAACAACACCAAATTTGTCCA
  TGATAATTACGTGATTCGGAGGACAGCTGTGGACAGTGGAATTGCTCTCCTCACCAATTTCCAGGTGACC
  AAACTTTTTGCTGAAGCTGTGCAGAAATCTCGCACTGTGGACTCCAAGAGTCTGTTCCACTACAGGCAGT
  ACAGTGCTGGAAAGGCCGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222149 representing NM_001080809
  Red=Cloning site Green=Tags(s)
  
  MTRILTACKVVKTLKSGFGFANVTTKRQWDFSRPGIRLLSVKAKTAHIVLEDGTKMKGYSFGHPSSVAGE
  VVFNTGLGGYPEALTDPAYKGQILTMANPIIGNGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSNDY
  NHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVK
  VFGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMEYDGLLIAGGPGNPALAQPLIQNVKK
  ILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITAQNHGYALDNTLPAGWK
  PLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFDSFFSLIKKGKGTTITSVLPKPALVASRV
  EVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQ
  FVTEVIKAERPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEK
  IAPSFAVESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLIDLGTKAFAMTNQILVERSVTGWK
  EIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSVNVVRHLGIVGECNIQ
  FALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMV
  TKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLK
  KELSEPSSTRIYAIAKALENNMSLDEIVRLTSIDKWFLYKMRDILNMDKTLKGLNSDSVTEETLRKAKEI
  GFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFDEHGIMVLG
  CGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACN
  GCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAN
  SVGYPCLLRPSYVLSGSAMNVVFSEDEMKRFLEEATRVSQEHPVVLTKFVEGAREVEMDAVGKEGRVISH
  AISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRA
  SRSFPFVSKTLGVDFIDVATKVMIGESIDEKRLPTLEQPIIPSDYVAIKAPMFSWPRLRDADPILRCEMA
  STGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNA
  NNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVT
  KLFAEAVQKSRTVDSKSLFHYRQYSAGKAA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080809
• ORF Size: 4500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080809.2, NP_001074278.1
• RefSeq Size: 4704 bp
• RefSeq ORF: 4503 bp
• Locus ID: 227231
• UniProt ID: Q8C196
• Cytogenetics: 1 33.75 cM
• Gene Summary: This gene encodes a protein localized to the inner mitochondrial matrix. The encoded protein plays a role in the detoxification of ammonia by catalyzing the first step in the urea cycle in which carbomyl-phosphate is synthesized from ammonia and bicarbonate. Carbamoyl-phosphate is subsequently converted to urea that is excreted by the kidneys. Deficiency of the encoded enzyme leads to an accumulation of ammonia in the blood. High levels of ammonia are toxic to the central nervous system and result in neurological disorders. [provided by RefSeq, Oct 2013]